BMRB Entry 52628

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52628

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Title:
p14ARF forms meso-scale assemblies upon phase separation with NPM1
Deposition date:
2024-10-04
Original release date:
2025-01-09
Authors:
Gibbs, Eric; Kriwacki, Richard
Citation:

Citation: Gibbs, Eric; Miao, Qi; Ferrolino, Mylene; Bajpai, Richa; Hassan, Aila; Phillips, Aaron; Pitre, Aaron; Kummerle, Rainer; Miller, Shondra; Nagy, Gergely; Leite, Wellington; Heller, William; Stanley, Chris; Perrone, Barbara; Kriwacki, Richard. "p14ARF forms meso-scale assemblies upon phase separation with NPM1"  Nat. Commun. 15, 9531-9531 (2024).
PubMed: 39528457

Assembly members:

Assembly members:
entity_1, polymer, 154 residues, Formula weight is not available
entity_2, polymer, 294 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSQDLENLYFQ GSMVRRFLVTLRIRRACGPP RVRVFVVHIPRLTGEWAAPG APAAVALVLMLLRSQRLGQQ PLPRRPGHDDGQRPSGGAAA APRRGAQLRRPRHSHPTRAR RCPGGLPGHAGGAAPGRGAA GRARCLGPSARGPG
entity_2: MEDSMDMDMSPLRPQNYLFG CELKADKDYHFKVDNDENEH QLSLRTVSLGAGAKDELHIV EAEAMNYEGSPIKVTLATLK MSVQPTVSLGGFEITPPVVL RLKCGSGPVHISGQHLVAVE EDAESEDEEEEDVKLLSISG KRSAPGGGSKVPQKKVKLAA DEDDDDDDEEDDDEDDDDDD FDDEEAEEKAPVKKSIRDTP AKNAQKSNQNGKDSKPSSTP RSKGQESFKKQEKTPKTPKG PSSVEDIKAKMQASIEKGGS LPKVEAKFINYVKNCFRMTD QEAIQDLWQWRKSL

Data sets:
Data typeCount
13C chemical shifts109
15N chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1p14ARF (state 1)1
2p14ARF (state 2)1
3NPM12

Entities:

Entity 1, p14ARF (state 1) 154 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERGLNASPLEUGLUASNLEUTYRPHEGLN
3   GLYSERMETVALARGARGPHELEUVALTHR
4   LEUARGILEARGARGALACYSGLYPROPRO
5   ARGVALARGVALPHEVALVALHISILEPRO
6   ARGLEUTHRGLYGLUTRPALAALAPROGLY
7   ALAPROALAALAVALALALEUVALLEUMET
8   LEULEUARGSERGLNARGLEUGLYGLNGLN
9   PROLEUPROARGARGPROGLYHISASPASP
10   GLYGLNARGPROSERGLYGLYALAALAALA
11   ALAPROARGARGGLYALAGLNLEUARGARG
12   PROARGHISSERHISPROTHRARGALAARG
13   ARGCYSPROGLYGLYLEUPROGLYHISALA
14   GLYGLYALAALAPROGLYARGGLYALAALA
15   GLYARGALAARGCYSLEUGLYPROSERALA
16   ARGGLYPROGLY

Entity 2, NPM1 294 residues - Formula weight is not available

1   METGLUASPSERMETASPMETASPMETSER
2   PROLEUARGPROGLNASNTYRLEUPHEGLY
3   CYSGLULEULYSALAASPLYSASPTYRHIS
4   PHELYSVALASPASNASPGLUASNGLUHIS
5   GLNLEUSERLEUARGTHRVALSERLEUGLY
6   ALAGLYALALYSASPGLULEUHISILEVAL
7   GLUALAGLUALAMETASNTYRGLUGLYSER
8   PROILELYSVALTHRLEUALATHRLEULYS
9   METSERVALGLNPROTHRVALSERLEUGLY
10   GLYPHEGLUILETHRPROPROVALVALLEU
11   ARGLEULYSCYSGLYSERGLYPROVALHIS
12   ILESERGLYGLNHISLEUVALALAVALGLU
13   GLUASPALAGLUSERGLUASPGLUGLUGLU
14   GLUASPVALLYSLEULEUSERILESERGLY
15   LYSARGSERALAPROGLYGLYGLYSERLYS
16   VALPROGLNLYSLYSVALLYSLEUALAALA
17   ASPGLUASPASPASPASPASPASPGLUGLU
18   ASPASPASPGLUASPASPASPASPASPASP
19   PHEASPASPGLUGLUALAGLUGLULYSALA
20   PROVALLYSLYSSERILEARGASPTHRPRO
21   ALALYSASNALAGLNLYSSERASNGLNASN
22   GLYLYSASPSERLYSPROSERSERTHRPRO
23   ARGSERLYSGLYGLNGLUSERPHELYSLYS
24   GLNGLULYSTHRPROLYSTHRPROLYSGLY
25   PROSERSERVALGLUASPILELYSALALYS
26   METGLNALASERILEGLULYSGLYGLYSER
27   LEUPROLYSVALGLUALALYSPHEILEASN
28   TYRVALLYSASNCYSPHEARGMETTHRASP
29   GLNGLUALAILEGLNASPLEUTRPGLNTRP
30   ARGLYSSERLEU