BMRB Entry 5221

Title:
Backbone 1H, 13C, and 15N resonance assignments for the C-Terminal Region of Ku86 (Ku86CTR)
Deposition date:
2001-11-30
Original release date:
2002-08-22
Authors:
Harris, Richard; Maman, Joseph; Hinks, John; Sankar, Andrew; Pearl, Laurence; Driscoll, Paul
Citation:

Citation: Harris, Richard; Maman, Joseph; Hinks, John; Sankar, Andrew; Pearl, Laurence; Driscoll, Paul. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments for the C-Terminal Region of Ku86 (Ku86CTR) "  J. Biomol. NMR 22, 373-374 (2002).
PubMed: 12018486

Assembly members:

Assembly members:
C-terminal region of Ku86, polymer, 184 residues, 20741 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
1H chemical shifts509
13C chemical shifts448
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ku86CTR1

Entities:

Entity 1, Ku86CTR 184 residues - 20741 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYMETALASERGLYTHRMETLYSLYSLYS
3   ASPGLNVALTHRALAGLNGLUILEPHEGLN
4   ASPASNHISGLUASPGLYPROTHRALALYS
5   LYSLEULYSTHRGLUGLNGLYGLYALAHIS
6   PHESERVALSERSERLEUALAGLUGLYSER
7   VALTHRSERVALGLYSERVALASNPROALA
8   GLUASNPHEARGVALLEUVALLYSGLNLYS
9   LYSALASERPHEGLUGLUALASERASNGLN
10   LEUILEASNHISILEGLUGLNPHELEUASP
11   THRASNGLUTHRPROTYRPHEMETLYSSER
12   ILEASPCYSILEARGALAPHEARGGLUGLU
13   ALAILELYSPHESERGLUGLUGLNARGPHE
14   ASNASNPHELEULYSALALEUGLNGLULYS
15   VALGLUILELYSGLNLEUASNHISPHETRP
16   GLUILEVALVALGLNASPGLYILETHRLEU
17   ILETHRLYSGLUGLUALASERGLYSERSER
18   VALTHRALAGLUGLUALALYSLYSPHELEU
19   ALAPROLYSASP

Samples:

sample_1: C-terminal region of Ku86, [U-13C; U-15N], 1.0 – 1.5 mM

cond_1: pH: 8.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-15N TROSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
H(CCO)NH-TOCSYnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available

Software:

nmrPIPE - data processing

ANSIG vv3.3 - spectral analysis

NMR spectrometers:

  • Varian UNITYplus 500 MHz
  • Varian UNITYplus 600 MHz

Related Database Links:

BMRB 5912
PDB
DBJ BAD96323 BAE01114 BAF83429 BAH24115
EMBL CAA40736 CAH92321
GB AAA36154 AAA59475 AAH19027 AAH95442 AAR11279
REF NP_001126362 NP_001244295 NP_066964 XP_001151873 XP_003254095
SP P13010
AlphaFold P13010

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks