BMRB Entry 50796

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50796

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Title:
Backbone assignment for yeast Yth1 zinc finger domain 4
Deposition date:
2021-03-01
Original release date:
2021-09-13
Authors:
Yu, Conny; Kumar, Ananthanarayanan; Rodriguez-Molina, Juan; Li, Xiaohan; Freund, Stefan; Passmore, Lori
Citation:

Citation: Kumar, Ananthanarayanan; Yu, Conny; Rodriguez-Molina, Juan; Li, Xiaohan; Freund, Stefan; Passmore, Lori. "Dynamics in Fip1 regulate eukaryotic mRNA 3' end processing"  Genes Dev. 35, 1510-1526 (2021).
PubMed: 34593603

Assembly members:

Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLGSMASKIPKCENYEMGF CPLGSSCPRRHIKKVFCQRY MTGFCPLGKD

Data sets:
Data typeCount
13C chemical shifts94
15N chemical shifts33
1H chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Yth1ZF41

Entities:

Entity 1, Yth1ZF4 50 residues - Formula weight is not available

1   GLYPROLEUGLYSERMETALASERLYSILE
2   PROLYSCYSGLUASNTYRGLUMETGLYPHE
3   CYSPROLEUGLYSERSERCYSPROARGARG
4   HISILELYSLYSVALPHECYSGLNARGTYR
5   METTHRGLYPHECYSPROLEUGLYLYSASP

Samples:

sample_1: Yth1ZF4, [U-100% 15N], 100 uM; HEPES 50 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CAN)NHsample_1isotropicsample_conditions_1
3D HN(COCA)NNHsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
3D H(CA)CONsample_1isotropicsample_conditions_1
3D H(CA)NCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.1 - collection, processing

NMRFAM-SPARKY v1.47 - chemical shift assignment, data analysis, peak picking

MddNMR - processing

MARS - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks