data_50796 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50796 _Entry.Title ; Backbone assignment for yeast Yth1 zinc finger domain 4 ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2021-03-01 _Entry.Accession_date 2021-03-01 _Entry.Last_release_date 2021-03-01 _Entry.Original_release_date 2021-03-01 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Conny Yu . W.H. . . 50796 2 Ananthanarayanan Kumar . . . . 50796 3 Juan Rodriguez-Molina . B. . . 50796 4 Xiaohan Li . . . . 50796 5 Stefan Freund . M.V. . . 50796 6 Lori Passmore . A. . . 50796 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 50796 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 94 50796 '15N chemical shifts' 33 50796 '1H chemical shifts' 32 50796 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 2 . . 2021-11-15 2021-03-01 update BMRB 'update entry citation' 50796 1 . . 2021-09-13 2021-03-01 original author 'original release' 50796 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 50795 'Backbone assignment for yeast Fip1 (1-226)' 50796 BMRB 50797 'Backbone assignment for yeast Yth1 zinc finger domain 4, 5 and C-terminal residues' 50796 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50796 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 34593603 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Dynamics in Fip1 regulate eukaryotic mRNA 3' end processing ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Genes Dev.' _Citation.Journal_name_full 'Genes & development' _Citation.Journal_volume 35 _Citation.Journal_issue 21-22 _Citation.Journal_ASTM . _Citation.Journal_ISSN 1549-5477 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1510 _Citation.Page_last 1526 _Citation.Year 2021 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Ananthanarayanan Kumar . . . . 50796 1 2 Conny Yu . W.H. . . 50796 1 3 Juan Rodriguez-Molina . B. . . 50796 1 4 Xiaohan Li . . . . 50796 1 5 Stefan Freund . M.V. . . 50796 1 6 Lori Passmore . A. . . 50796 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50796 _Assembly.ID 1 _Assembly.Name Yth1ZF4 _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 Yth1ZF4 1 $entity_1 . . yes native no no . . . 50796 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50796 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GPLGSMASKIPKCENYEMGF CPLGSSCPRRHIKKVFCQRY MTGFCPLGKD ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 50 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 112 GLY . 50796 1 2 113 PRO . 50796 1 3 114 LEU . 50796 1 4 115 GLY . 50796 1 5 116 SER . 50796 1 6 117 MET . 50796 1 7 118 ALA . 50796 1 8 119 SER . 50796 1 9 120 LYS . 50796 1 10 121 ILE . 50796 1 11 122 PRO . 50796 1 12 123 LYS . 50796 1 13 124 CYS . 50796 1 14 125 GLU . 50796 1 15 126 ASN . 50796 1 16 127 TYR . 50796 1 17 128 GLU . 50796 1 18 129 MET . 50796 1 19 130 GLY . 50796 1 20 131 PHE . 50796 1 21 132 CYS . 50796 1 22 133 PRO . 50796 1 23 134 LEU . 50796 1 24 135 GLY . 50796 1 25 136 SER . 50796 1 26 137 SER . 50796 1 27 138 CYS . 50796 1 28 139 PRO . 50796 1 29 140 ARG . 50796 1 30 141 ARG . 50796 1 31 142 HIS . 50796 1 32 143 ILE . 50796 1 33 144 LYS . 50796 1 34 145 LYS . 50796 1 35 146 VAL . 50796 1 36 147 PHE . 50796 1 37 148 CYS . 50796 1 38 149 GLN . 50796 1 39 150 ARG . 50796 1 40 151 TYR . 50796 1 41 152 MET . 50796 1 42 153 THR . 50796 1 43 154 GLY . 50796 1 44 155 PHE . 50796 1 45 156 CYS . 50796 1 46 157 PRO . 50796 1 47 158 LEU . 50796 1 48 159 GLY . 50796 1 49 160 LYS . 50796 1 50 161 ASP . 50796 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 50796 1 . PRO 2 2 50796 1 . LEU 3 3 50796 1 . GLY 4 4 50796 1 . SER 5 5 50796 1 . MET 6 6 50796 1 . ALA 7 7 50796 1 . SER 8 8 50796 1 . LYS 9 9 50796 1 . ILE 10 10 50796 1 . PRO 11 11 50796 1 . LYS 12 12 50796 1 . CYS 13 13 50796 1 . GLU 14 14 50796 1 . ASN 15 15 50796 1 . TYR 16 16 50796 1 . GLU 17 17 50796 1 . MET 18 18 50796 1 . GLY 19 19 50796 1 . PHE 20 20 50796 1 . CYS 21 21 50796 1 . PRO 22 22 50796 1 . LEU 23 23 50796 1 . GLY 24 24 50796 1 . SER 25 25 50796 1 . SER 26 26 50796 1 . CYS 27 27 50796 1 . PRO 28 28 50796 1 . ARG 29 29 50796 1 . ARG 30 30 50796 1 . HIS 31 31 50796 1 . ILE 32 32 50796 1 . LYS 33 33 50796 1 . LYS 34 34 50796 1 . VAL 35 35 50796 1 . PHE 36 36 50796 1 . CYS 37 37 50796 1 . GLN 38 38 50796 1 . ARG 39 39 50796 1 . TYR 40 40 50796 1 . MET 41 41 50796 1 . THR 42 42 50796 1 . GLY 43 43 50796 1 . PHE 44 44 50796 1 . CYS 45 45 50796 1 . PRO 46 46 50796 1 . LEU 47 47 50796 1 . GLY 48 48 50796 1 . LYS 49 49 50796 1 . ASP 50 50 50796 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50796 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 4932 organism . 'Saccharomyces cerevisiae' "baker's yeast" . . Eukaryota Fungi Saccharomyces cerevisiae . . . . . . . . . . . . . 50796 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50796 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . plasmid . . pGEX6P-2 . . . 50796 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50796 _Sample.ID 1 _Sample.Name Yth1ZF4 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '95% H2O/5% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 Yth1ZF4 '[U-100% 15N]' . . 1 $entity_1 . . 100 . . uM . . . . 50796 1 2 HEPES 'natural abundance' . . . . . . 50 . . mM . . . . 50796 1 3 NaCl 'natural abundance' . . . . . . 150 . . mM . . . . 50796 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50796 _Sample_condition_list.ID 1 _Sample_condition_list.Name Yth1ZF4 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 150 . mM 50796 1 pH 7.4 . pH 50796 1 pressure 1 . atm 50796 1 temperature 298 . K 50796 1 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50796 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 3.6.1 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 50796 1 processing . 50796 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50796 _Software.ID 2 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version 1.47 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 50796 2 'data analysis' . 50796 2 'peak picking' . 50796 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 50796 _Software.ID 3 _Software.Type . _Software.Name MddNMR _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID processing . 50796 3 stop_ save_ save_software_4 _Software.Sf_category software _Software.Sf_framecode software_4 _Software.Entry_ID 50796 _Software.ID 4 _Software.Type . _Software.Name MARS _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 50796 4 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50796 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name '700 MHz Avance II+' _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE III' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 700 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50796 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '3D HNCO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 2 '3D HN(CA)CO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 3 '3D HNCA' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 4 '3D HNCACB' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 5 '3D HN(CO)CACB' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 6 '3D HN(CAN)NH' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 7 '3D HN(COCA)NNH' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 8 '2D CON' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 9 '3D H(CA)CON' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 10 '3D H(CA)NCO' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50796 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 50796 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'CS Reference' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 water protons . . . . ppm 4.7 internal indirect . . . . . . 50796 1 H 1 water protons . . . . ppm 4.7 internal direct 1 . . . . . 50796 1 N 15 water protons . . . . ppm 4.7 internal indirect . . . . . . 50796 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 50796 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name Yth1ZF4 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '3D HNCO' . . . 50796 1 2 '3D HN(CA)CO' . . . 50796 1 3 '3D HNCA' . . . 50796 1 4 '3D HNCACB' . . . 50796 1 5 '3D HN(CO)CACB' . . . 50796 1 8 '2D CON' . . . 50796 1 9 '3D H(CA)CON' . . . 50796 1 10 '3D H(CA)NCO' . . . 50796 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 1 $software_1 . . 50796 1 2 $software_2 . . 50796 1 3 $software_3 . . 50796 1 4 $software_4 . . 50796 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 7 7 ALA C C 13 177.00 . . . . . . . . 118 ALA C . 50796 1 2 . 1 . 1 7 7 ALA CA C 13 52.01 . . . . . . . . 118 ALA CA . 50796 1 3 . 1 . 1 7 7 ALA CB C 13 18.20 . . . . . . . . 118 ALA CB . 50796 1 4 . 1 . 1 7 7 ALA N N 15 125.47 . . . . . . . . 118 ALA N . 50796 1 5 . 1 . 1 8 8 SER H H 1 8.00 . . . . . . . . 119 SER H . 50796 1 6 . 1 . 1 8 8 SER C C 13 173.69 . . . . . . . . 119 SER C . 50796 1 7 . 1 . 1 8 8 SER CA C 13 57.41 . . . . . . . . 119 SER CA . 50796 1 8 . 1 . 1 8 8 SER CB C 13 63.01 . . . . . . . . 119 SER CB . 50796 1 9 . 1 . 1 8 8 SER N N 15 115.52 . . . . . . . . 119 SER N . 50796 1 10 . 1 . 1 9 9 LYS H H 1 8.02 . . . . . . . . 120 LYS H . 50796 1 11 . 1 . 1 9 9 LYS C C 13 175.63 . . . . . . . . 120 LYS C . 50796 1 12 . 1 . 1 9 9 LYS CA C 13 55.26 . . . . . . . . 120 LYS CA . 50796 1 13 . 1 . 1 9 9 LYS CB C 13 31.86 . . . . . . . . 120 LYS CB . 50796 1 14 . 1 . 1 9 9 LYS N N 15 123.47 . . . . . . . . 120 LYS N . 50796 1 15 . 1 . 1 10 10 ILE H H 1 7.92 . . . . . . . . 121 ILE H . 50796 1 16 . 1 . 1 10 10 ILE C C 13 173.66 . . . . . . . . 121 ILE C . 50796 1 17 . 1 . 1 10 10 ILE CA C 13 57.83 . . . . . . . . 121 ILE CA . 50796 1 18 . 1 . 1 10 10 ILE CB C 13 37.52 . . . . . . . . 121 ILE CB . 50796 1 19 . 1 . 1 10 10 ILE N N 15 124.60 . . . . . . . . 121 ILE N . 50796 1 20 . 1 . 1 14 14 GLU H H 1 9.24 . . . . . . . . 125 GLU H . 50796 1 21 . 1 . 1 14 14 GLU C C 13 177.41 . . . . . . . . 125 GLU C . 50796 1 22 . 1 . 1 14 14 GLU CA C 13 58.70 . . . . . . . . 125 GLU CA . 50796 1 23 . 1 . 1 14 14 GLU CB C 13 28.30 . . . . . . . . 125 GLU CB . 50796 1 24 . 1 . 1 14 14 GLU N N 15 133.37 . . . . . . . . 125 GLU N . 50796 1 25 . 1 . 1 15 15 ASN H H 1 7.75 . . . . . . . . 126 ASN H . 50796 1 26 . 1 . 1 15 15 ASN C C 13 176.01 . . . . . . . . 126 ASN C . 50796 1 27 . 1 . 1 15 15 ASN CA C 13 55.38 . . . . . . . . 126 ASN CA . 50796 1 28 . 1 . 1 15 15 ASN CB C 13 36.56 . . . . . . . . 126 ASN CB . 50796 1 29 . 1 . 1 15 15 ASN N N 15 119.21 . . . . . . . . 126 ASN N . 50796 1 30 . 1 . 1 16 16 TYR H H 1 8.93 . . . . . . . . 127 TYR H . 50796 1 31 . 1 . 1 16 16 TYR C C 13 178.33 . . . . . . . . 127 TYR C . 50796 1 32 . 1 . 1 16 16 TYR CA C 13 61.38 . . . . . . . . 127 TYR CA . 50796 1 33 . 1 . 1 16 16 TYR N N 15 125.21 . . . . . . . . 127 TYR N . 50796 1 34 . 1 . 1 17 17 GLU H H 1 7.56 . . . . . . . . 128 GLU H . 50796 1 35 . 1 . 1 17 17 GLU C C 13 176.91 . . . . . . . . 128 GLU C . 50796 1 36 . 1 . 1 17 17 GLU CA C 13 58.21 . . . . . . . . 128 GLU CA . 50796 1 37 . 1 . 1 17 17 GLU CB C 13 28.93 . . . . . . . . 128 GLU CB . 50796 1 38 . 1 . 1 17 17 GLU N N 15 118.90 . . . . . . . . 128 GLU N . 50796 1 39 . 1 . 1 18 18 MET H H 1 7.25 . . . . . . . . 129 MET H . 50796 1 40 . 1 . 1 18 18 MET C C 13 175.92 . . . . . . . . 129 MET C . 50796 1 41 . 1 . 1 18 18 MET CA C 13 55.02 . . . . . . . . 129 MET CA . 50796 1 42 . 1 . 1 18 18 MET CB C 13 31.20 . . . . . . . . 129 MET CB . 50796 1 43 . 1 . 1 18 18 MET N N 15 116.22 . . . . . . . . 129 MET N . 50796 1 44 . 1 . 1 19 19 GLY H H 1 8.10 . . . . . . . . 130 GLY H . 50796 1 45 . 1 . 1 19 19 GLY C C 13 172.91 . . . . . . . . 130 GLY C . 50796 1 46 . 1 . 1 19 19 GLY CA C 13 44.31 . . . . . . . . 130 GLY CA . 50796 1 47 . 1 . 1 19 19 GLY N N 15 110.17 . . . . . . . . 130 GLY N . 50796 1 48 . 1 . 1 20 20 PHE H H 1 7.39 . . . . . . . . 131 PHE H . 50796 1 49 . 1 . 1 20 20 PHE C C 13 170.85 . . . . . . . . 131 PHE C . 50796 1 50 . 1 . 1 20 20 PHE CA C 13 57.41 . . . . . . . . 131 PHE CA . 50796 1 51 . 1 . 1 20 20 PHE CB C 13 39.51 . . . . . . . . 131 PHE CB . 50796 1 52 . 1 . 1 20 20 PHE N N 15 119.90 . . . . . . . . 131 PHE N . 50796 1 53 . 1 . 1 24 24 GLY H H 1 8.19 . . . . . . . . 135 GLY H . 50796 1 54 . 1 . 1 24 24 GLY C C 13 173.23 . . . . . . . . 135 GLY C . 50796 1 55 . 1 . 1 24 24 GLY CA C 13 44.29 . . . . . . . . 135 GLY CA . 50796 1 56 . 1 . 1 24 24 GLY N N 15 110.57 . . . . . . . . 135 GLY N . 50796 1 57 . 1 . 1 25 25 SER H H 1 7.94 . . . . . . . . 136 SER H . 50796 1 58 . 1 . 1 25 25 SER C C 13 173.84 . . . . . . . . 136 SER C . 50796 1 59 . 1 . 1 25 25 SER CA C 13 57.73 . . . . . . . . 136 SER CA . 50796 1 60 . 1 . 1 25 25 SER CB C 13 62.81 . . . . . . . . 136 SER CB . 50796 1 61 . 1 . 1 25 25 SER N N 15 114.91 . . . . . . . . 136 SER N . 50796 1 62 . 1 . 1 26 26 SER H H 1 7.70 . . . . . . . . 137 SER H . 50796 1 63 . 1 . 1 26 26 SER C C 13 172.35 . . . . . . . . 137 SER C . 50796 1 64 . 1 . 1 26 26 SER CA C 13 57.46 . . . . . . . . 137 SER CA . 50796 1 65 . 1 . 1 26 26 SER CB C 13 62.52 . . . . . . . . 137 SER CB . 50796 1 66 . 1 . 1 26 26 SER N N 15 116.31 . . . . . . . . 137 SER N . 50796 1 67 . 1 . 1 27 27 CYS H H 1 7.12 . . . . . . . . 138 CYS H . 50796 1 68 . 1 . 1 27 27 CYS C C 13 174.62 . . . . . . . . 138 CYS C . 50796 1 69 . 1 . 1 27 27 CYS CA C 13 57.07 . . . . . . . . 138 CYS CA . 50796 1 70 . 1 . 1 27 27 CYS CB C 13 30.11 . . . . . . . . 138 CYS CB . 50796 1 71 . 1 . 1 27 27 CYS N N 15 127.84 . . . . . . . . 138 CYS N . 50796 1 72 . 1 . 1 29 29 ARG H H 1 8.30 . . . . . . . . 140 ARG H . 50796 1 73 . 1 . 1 29 29 ARG C C 13 175.30 . . . . . . . . 140 ARG C . 50796 1 74 . 1 . 1 29 29 ARG CA C 13 55.33 . . . . . . . . 140 ARG CA . 50796 1 75 . 1 . 1 29 29 ARG CB C 13 29.58 . . . . . . . . 140 ARG CB . 50796 1 76 . 1 . 1 29 29 ARG N N 15 122.05 . . . . . . . . 140 ARG N . 50796 1 77 . 1 . 1 30 30 ARG H H 1 7.89 . . . . . . . . 141 ARG H . 50796 1 78 . 1 . 1 30 30 ARG C C 13 175.41 . . . . . . . . 141 ARG C . 50796 1 79 . 1 . 1 30 30 ARG CA C 13 55.50 . . . . . . . . 141 ARG CA . 50796 1 80 . 1 . 1 30 30 ARG CB C 13 29.68 . . . . . . . . 141 ARG CB . 50796 1 81 . 1 . 1 30 30 ARG N N 15 122.24 . . . . . . . . 141 ARG N . 50796 1 82 . 1 . 1 32 32 ILE H H 1 7.91 . . . . . . . . 143 ILE H . 50796 1 83 . 1 . 1 32 32 ILE C C 13 173.40 . . . . . . . . 143 ILE C . 50796 1 84 . 1 . 1 32 32 ILE CA C 13 57.61 . . . . . . . . 143 ILE CA . 50796 1 85 . 1 . 1 32 32 ILE CB C 13 37.82 . . . . . . . . 143 ILE CB . 50796 1 86 . 1 . 1 32 32 ILE N N 15 125.59 . . . . . . . . 143 ILE N . 50796 1 87 . 1 . 1 33 33 LYS H H 1 8.29 . . . . . . . . 144 LYS H . 50796 1 88 . 1 . 1 33 33 LYS C C 13 175.55 . . . . . . . . 144 LYS C . 50796 1 89 . 1 . 1 33 33 LYS CA C 13 55.69 . . . . . . . . 144 LYS CA . 50796 1 90 . 1 . 1 33 33 LYS CB C 13 32.27 . . . . . . . . 144 LYS CB . 50796 1 91 . 1 . 1 33 33 LYS N N 15 128.51 . . . . . . . . 144 LYS N . 50796 1 92 . 1 . 1 34 34 LYS H H 1 8.32 . . . . . . . . 145 LYS H . 50796 1 93 . 1 . 1 34 34 LYS C C 13 175.30 . . . . . . . . 145 LYS C . 50796 1 94 . 1 . 1 34 34 LYS CA C 13 55.52 . . . . . . . . 145 LYS CA . 50796 1 95 . 1 . 1 34 34 LYS CB C 13 32.34 . . . . . . . . 145 LYS CB . 50796 1 96 . 1 . 1 34 34 LYS N N 15 125.43 . . . . . . . . 145 LYS N . 50796 1 97 . 1 . 1 35 35 VAL H H 1 7.94 . . . . . . . . 146 VAL H . 50796 1 98 . 1 . 1 35 35 VAL C C 13 174.81 . . . . . . . . 146 VAL C . 50796 1 99 . 1 . 1 35 35 VAL CA C 13 61.12 . . . . . . . . 146 VAL CA . 50796 1 100 . 1 . 1 35 35 VAL CB C 13 32.34 . . . . . . . . 146 VAL CB . 50796 1 101 . 1 . 1 35 35 VAL N N 15 122.65 . . . . . . . . 146 VAL N . 50796 1 102 . 1 . 1 36 36 PHE H H 1 8.24 . . . . . . . . 147 PHE H . 50796 1 103 . 1 . 1 36 36 PHE C C 13 174.48 . . . . . . . . 147 PHE C . 50796 1 104 . 1 . 1 36 36 PHE CA C 13 56.95 . . . . . . . . 147 PHE CA . 50796 1 105 . 1 . 1 36 36 PHE CB C 13 39.03 . . . . . . . . 147 PHE CB . 50796 1 106 . 1 . 1 36 36 PHE N N 15 125.30 . . . . . . . . 147 PHE N . 50796 1 107 . 1 . 1 38 38 GLN H H 1 8.21 . . . . . . . . 149 GLN H . 50796 1 108 . 1 . 1 38 38 GLN C C 13 174.88 . . . . . . . . 149 GLN C . 50796 1 109 . 1 . 1 38 38 GLN CA C 13 55.33 . . . . . . . . 149 GLN CA . 50796 1 110 . 1 . 1 38 38 GLN CB C 13 28.39 . . . . . . . . 149 GLN CB . 50796 1 111 . 1 . 1 38 38 GLN N N 15 124.30 . . . . . . . . 149 GLN N . 50796 1 112 . 1 . 1 39 39 ARG H H 1 8.08 . . . . . . . . 150 ARG H . 50796 1 113 . 1 . 1 39 39 ARG C C 13 175.02 . . . . . . . . 150 ARG C . 50796 1 114 . 1 . 1 39 39 ARG CA C 13 55.50 . . . . . . . . 150 ARG CA . 50796 1 115 . 1 . 1 39 39 ARG CB C 13 30.02 . . . . . . . . 150 ARG CB . 50796 1 116 . 1 . 1 39 39 ARG N N 15 122.58 . . . . . . . . 150 ARG N . 50796 1 117 . 1 . 1 40 40 TYR H H 1 7.91 . . . . . . . . 151 TYR H . 50796 1 118 . 1 . 1 40 40 TYR C C 13 175.03 . . . . . . . . 151 TYR C . 50796 1 119 . 1 . 1 40 40 TYR CA C 13 56.76 . . . . . . . . 151 TYR CA . 50796 1 120 . 1 . 1 40 40 TYR CB C 13 37.77 . . . . . . . . 151 TYR CB . 50796 1 121 . 1 . 1 40 40 TYR N N 15 120.65 . . . . . . . . 151 TYR N . 50796 1 122 . 1 . 1 41 41 MET H H 1 8.02 . . . . . . . . 152 MET H . 50796 1 123 . 1 . 1 41 41 MET C C 13 175.33 . . . . . . . . 152 MET C . 50796 1 124 . 1 . 1 41 41 MET CA C 13 54.70 . . . . . . . . 152 MET CA . 50796 1 125 . 1 . 1 41 41 MET CB C 13 32.05 . . . . . . . . 152 MET CB . 50796 1 126 . 1 . 1 41 41 MET N N 15 122.20 . . . . . . . . 152 MET N . 50796 1 127 . 1 . 1 42 42 THR H H 1 7.89 . . . . . . . . 153 THR H . 50796 1 128 . 1 . 1 42 42 THR C C 13 174.12 . . . . . . . . 153 THR C . 50796 1 129 . 1 . 1 42 42 THR CA C 13 61.63 . . . . . . . . 153 THR CA . 50796 1 130 . 1 . 1 42 42 THR CB C 13 68.92 . . . . . . . . 153 THR CB . 50796 1 131 . 1 . 1 42 42 THR N N 15 115.22 . . . . . . . . 153 THR N . 50796 1 132 . 1 . 1 43 43 GLY H H 1 8.18 . . . . . . . . 154 GLY H . 50796 1 133 . 1 . 1 43 43 GLY C C 13 172.76 . . . . . . . . 154 GLY C . 50796 1 134 . 1 . 1 43 43 GLY CA C 13 44.24 . . . . . . . . 154 GLY CA . 50796 1 135 . 1 . 1 43 43 GLY N N 15 111.92 . . . . . . . . 154 GLY N . 50796 1 136 . 1 . 1 44 44 PHE H H 1 7.86 . . . . . . . . 155 PHE H . 50796 1 137 . 1 . 1 44 44 PHE C C 13 174.13 . . . . . . . . 155 PHE C . 50796 1 138 . 1 . 1 44 44 PHE CA C 13 56.95 . . . . . . . . 155 PHE CA . 50796 1 139 . 1 . 1 44 44 PHE CB C 13 38.98 . . . . . . . . 155 PHE CB . 50796 1 140 . 1 . 1 44 44 PHE N N 15 120.66 . . . . . . . . 155 PHE N . 50796 1 141 . 1 . 1 45 45 CYS H H 1 8.00 . . . . . . . . 156 CYS H . 50796 1 142 . 1 . 1 45 45 CYS C C 13 171.50 . . . . . . . . 156 CYS C . 50796 1 143 . 1 . 1 45 45 CYS CA C 13 55.33 . . . . . . . . 156 CYS CA . 50796 1 144 . 1 . 1 45 45 CYS CB C 13 26.92 . . . . . . . . 156 CYS CB . 50796 1 145 . 1 . 1 45 45 CYS N N 15 124.87 . . . . . . . . 156 CYS N . 50796 1 146 . 1 . 1 47 47 LEU H H 1 7.72 . . . . . . . . 158 LEU H . 50796 1 147 . 1 . 1 47 47 LEU C C 13 177.71 . . . . . . . . 158 LEU C . 50796 1 148 . 1 . 1 47 47 LEU CA C 13 54.82 . . . . . . . . 158 LEU CA . 50796 1 149 . 1 . 1 47 47 LEU CB C 13 40.97 . . . . . . . . 158 LEU CB . 50796 1 150 . 1 . 1 47 47 LEU N N 15 119.76 . . . . . . . . 158 LEU N . 50796 1 151 . 1 . 1 48 48 GLY H H 1 7.26 . . . . . . . . 159 GLY H . 50796 1 152 . 1 . 1 48 48 GLY C C 13 174.73 . . . . . . . . 159 GLY C . 50796 1 153 . 1 . 1 48 48 GLY CA C 13 46.13 . . . . . . . . 159 GLY CA . 50796 1 154 . 1 . 1 48 48 GLY N N 15 109.23 . . . . . . . . 159 GLY N . 50796 1 155 . 1 . 1 50 50 ASP H H 1 8.27 . . . . . . . . 161 ASP H . 50796 1 156 . 1 . 1 50 50 ASP C C 13 175.53 . . . . . . . . 161 ASP C . 50796 1 157 . 1 . 1 50 50 ASP CA C 13 53.56 . . . . . . . . 161 ASP CA . 50796 1 158 . 1 . 1 50 50 ASP CB C 13 40.24 . . . . . . . . 161 ASP CB . 50796 1 159 . 1 . 1 50 50 ASP N N 15 121.55 . . . . . . . . 161 ASP N . 50796 1 stop_ save_