BMRB Entry 50710

Name: RGG FUS
Published: 2021-11-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50710

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RGG FUS

Deposition date:

2021-01-15

Original release date:

2021-11-14

Authors:

Mompean, Miguel; Laurents, Douglas

Citation:

Citation: Mompean, Miguel; Oroz, Javier; Laurents, Douglas. "Do polyproline II helix associations modulate biomolecular condensates?" FEBS Open Bio 11, 2390-2399 (2021).
PubMed: 33934561

Assembly members:

Assembly members:
entity_1, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RGGRGGYDRGGYRGGRGGDR GNYRGGRGGD

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	61
1H chemical shifts	410

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RGG FUS	1

Entities:

Entity 1, RGG FUS 30 residues - Formula weight is not available

1	ARG	GLY	GLY	ARG	GLY	GLY	TYR	ASP	ARG	GLY
2	GLY	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP	ARG
3	GLY	ASN	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP

Samples:

sample_1: RGG FUS 3.1 ± 0.2 mM; DSS 0.05 ± 0.05 mM; H2O 90 ± 1 %; D2O 10 ± 1 %

sample_2: RGG FUS 3.1 ± 0.2 mM; DSS 0.05 ± 0.05 mM; H2O 90 ± 1 %; D2O 10 ± 1 %; sodium carbonate 5 ± 1 mM

sample_3: RGG FUS 3.1 ± 0.2 mM; DSS 0.05 ± 0.05 mM; H2O 90 ± 1 %; D2O 10 ± 1 %; sodium carbonate 10 ± 2 mM

sample_conditions_1: ionic strength: 3 mM; pH: 2.53; pressure: 0.92 atm; temperature: 278.2 K

sample_conditions_2: ionic strength: 13 mM; pH: 7.25; pressure: 0.92 atm; temperature: 278.2 K

sample_conditions_3: ionic strength: 23 mM; pH: 9.96; pressure: 0.92 atm; temperature: 278.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
1D 1H	sample_2	isotropic	sample_conditions_2
2D 1H-1H COSY	sample_2	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_3

Software:

TOPSPIN v4.0.8 - collection, processing

NMRFAM-SPARKY v3.13 - chemical shift assignment, peak picking

CYANA - structure solution

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

1	ARG	GLY	GLY	ARG	GLY	GLY	TYR	ASP	ARG	GLY
2	GLY	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP	ARG
3	GLY	ASN	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP

1	ARG	GLY	GLY	ARG	GLY	GLY	TYR	ASP	ARG	GLY
2	GLY	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP	ARG
3	GLY	ASN	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP

1	ARG	GLY	GLY	ARG	GLY	GLY	TYR	ASP	ARG	GLY
2	GLY	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP	ARG
3	GLY	ASN	TYR	ARG	GLY	GLY	ARG	GLY	GLY	ASP