data_50710 ####################### # Entry information # ####################### save_entry_information_1 _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information_1 _Entry.ID 50710 _Entry.Title ; RGG FUS ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2021-01-15 _Entry.Accession_date 2021-01-15 _Entry.Last_release_date 2021-01-17 _Entry.Original_release_date 2021-01-17 _Entry.Origination author _Entry.Format_name . _Entry.NMR_STAR_version 3.2.14.0 _Entry.NMR_STAR_dict_location . _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Source_data_format . _Entry.Source_data_format_version . _Entry.Generated_software_name . _Entry.Generated_software_version . _Entry.Generated_software_ID . _Entry.Generated_software_label . _Entry.Generated_date . _Entry.DOI . _Entry.UUID . _Entry.Related_coordinate_file_name . _Entry.Details '30-mer peptide based on the third RGG region of Fus protein' _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.ORCID _Entry_author.Entry_ID 1 Miguel Mompean . . . 0000-0002-5608-3648 50710 2 Douglas Laurents . V. . 0000-0002-4187-165X 50710 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 3 50710 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 61 50710 '1H chemical shifts' 410 50710 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2021-11-14 . original BMRB . 50710 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 50709 'RGG Mini' 50710 stop_ save_ ############### # Citations # ############### save_citations_1 _Citation.Sf_category citations _Citation.Sf_framecode citations_1 _Citation.Entry_ID 50710 _Citation.ID 1 _Citation.Name . _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.PubMed_ID 33934561 _Citation.DOI . _Citation.Full_citation . _Citation.Title ; Do polyproline II helix associations modulate biomolecular condensates? ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'FEBS Open Bio' _Citation.Journal_name_full 'FEBS open bio' _Citation.Journal_volume 11 _Citation.Journal_issue 9 _Citation.Journal_ASTM . _Citation.Journal_ISSN 2211-5463 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 2390 _Citation.Page_last 2399 _Citation.Year 2021 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.ORCID _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Miguel Mompean . . . . 50710 1 2 Javier Oroz . . . . 50710 1 3 Douglas Laurents . V. . . 50710 1 stop_ loop_ _Citation_keyword.Keyword _Citation_keyword.Entry_ID _Citation_keyword.Citation_ID 'cation - pi interactions, polyproline II helix, Fused in Sarcoma protein' 50710 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly_1 _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly_1 _Assembly.Entry_ID 50710 _Assembly.ID 1 _Assembly.Name 'RGG FUS' _Assembly.BMRB_code . _Assembly.Number_of_components 1 _Assembly.Organic_ligands 0 _Assembly.Metal_ions 0 _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states no _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange no _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'RGG FUS' 1 $entity_1 . . yes native no no . . . 50710 1 stop_ loop_ _Assembly_bio_function.Biological_function _Assembly_bio_function.Entry_ID _Assembly_bio_function.Assembly_ID 'This is a peptide related to the third RGG segment of FUS protein from higher vertebrates.' 50710 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 50710 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name entity_1 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; RGGRGGYDRGGYRGGRGGDR GNYRGGRGGD ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 30 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . ARG . 50710 1 2 . GLY . 50710 1 3 . GLY . 50710 1 4 . ARG . 50710 1 5 . GLY . 50710 1 6 . GLY . 50710 1 7 . TYR . 50710 1 8 . ASP . 50710 1 9 . ARG . 50710 1 10 . GLY . 50710 1 11 . GLY . 50710 1 12 . TYR . 50710 1 13 . ARG . 50710 1 14 . GLY . 50710 1 15 . GLY . 50710 1 16 . ARG . 50710 1 17 . GLY . 50710 1 18 . GLY . 50710 1 19 . ASP . 50710 1 20 . ARG . 50710 1 21 . GLY . 50710 1 22 . ASN . 50710 1 23 . TYR . 50710 1 24 . ARG . 50710 1 25 . GLY . 50710 1 26 . GLY . 50710 1 27 . ARG . 50710 1 28 . GLY . 50710 1 29 . GLY . 50710 1 30 . ASP . 50710 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . ARG 1 1 50710 1 . GLY 2 2 50710 1 . GLY 3 3 50710 1 . ARG 4 4 50710 1 . GLY 5 5 50710 1 . GLY 6 6 50710 1 . TYR 7 7 50710 1 . ASP 8 8 50710 1 . ARG 9 9 50710 1 . GLY 10 10 50710 1 . GLY 11 11 50710 1 . TYR 12 12 50710 1 . ARG 13 13 50710 1 . GLY 14 14 50710 1 . GLY 15 15 50710 1 . ARG 16 16 50710 1 . GLY 17 17 50710 1 . GLY 18 18 50710 1 . ASP 19 19 50710 1 . ARG 20 20 50710 1 . GLY 21 21 50710 1 . ASN 22 22 50710 1 . TYR 23 23 50710 1 . ARG 24 24 50710 1 . GLY 25 25 50710 1 . GLY 26 26 50710 1 . ARG 27 27 50710 1 . GLY 28 28 50710 1 . GLY 29 29 50710 1 . ASP 30 30 50710 1 stop_ save_ #################### # Natural source # #################### save_natural_source_1 _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source_1 _Entity_natural_src_list.Entry_ID 50710 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Details _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . 'Related to, but not identical to the human sequence' 50710 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source_1 _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source_1 _Entity_experimental_src_list.Entry_ID 50710 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Details _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'chemical synthesis' . . . . . . . . . . . . . . . . 50710 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 50710 _Sample.ID 1 _Sample.Name 'pH 3' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'pH 2.53' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'RGG FUS' 'natural abundance' . . 1 $entity_1 . . 3.1 . . mM 0.2 . . . 50710 1 2 DSS 'natural abundance' . . . . . . 0.05 . . mM 0.05 . . . 50710 1 3 H2O 'natural abundance' . . . . . . 90 . . % 1 . . . 50710 1 4 D2O 'natural abundance' . . . . . . 10 . . % 1 . . . 50710 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 50710 _Sample.ID 2 _Sample.Name 'pH 7' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'pH 7.25' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'RGG FUS' 'natural abundance' . . 1 $entity_1 . . 3.1 . . mM 0.2 . . . 50710 2 2 DSS 'natural abundance' . . . . . . 0.05 . . mM 0.05 . . . 50710 2 3 H2O 'natural abundance' . . . . . . 90 . . % 1 . . . 50710 2 4 D2O 'natural abundance' . . . . . . 10 . . % 1 . . . 50710 2 5 'sodium carbonate' 'natural abundance' . . . . . . 5 . . mM 1 . . . 50710 2 stop_ save_ save_sample_3 _Sample.Sf_category sample _Sample.Sf_framecode sample_3 _Sample.Entry_ID 50710 _Sample.ID 3 _Sample.Name 'pH 10' _Sample.Type solution _Sample.Sub_type . _Sample.Details 'pH 9.96' _Sample.Aggregate_sample_number 1 _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'RGG FUS' 'natural abundance' . . 1 $entity_1 . . 3.1 . . mM 0.2 . . . 50710 3 2 DSS 'natural abundance' . . . . . . 0.05 . . mM 0.05 . . . 50710 3 3 H2O 'natural abundance' . . . . . . 90 . . % 1 . . . 50710 3 4 D2O 'natural abundance' . . . . . . 10 . . % 1 . . . 50710 3 5 'sodium carbonate' 'natural abundance' . . . . . . 10 . . mM 2 . . . 50710 3 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 50710 _Sample_condition_list.ID 1 _Sample_condition_list.Name 'pH 3' _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 3 1 mM 50710 1 pH 2.53 0.02 pH 50710 1 pressure 0.92 0.01 atm 50710 1 temperature 278.2 0.1 K 50710 1 stop_ save_ save_sample_conditions_2 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_2 _Sample_condition_list.Entry_ID 50710 _Sample_condition_list.ID 2 _Sample_condition_list.Name 'pH 7' _Sample_condition_list.Details 'pH 7.25' loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 13 2 mM 50710 2 pH 7.25 0.02 pH 50710 2 pressure 0.92 0.01 atm 50710 2 temperature 278.2 0.1 K 50710 2 stop_ save_ save_sample_conditions_3 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_3 _Sample_condition_list.Entry_ID 50710 _Sample_condition_list.ID 3 _Sample_condition_list.Name 'pH 10' _Sample_condition_list.Details 'pH 9.96' loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 23 3 mM 50710 3 pH 9.96 0.02 pH 50710 3 pressure 0.92 0.01 atm 50710 3 temperature 278.2 0.1 K 50710 3 stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Software.Sf_category software _Software.Sf_framecode software_1 _Software.Entry_ID 50710 _Software.ID 1 _Software.Type . _Software.Name TOPSPIN _Software.Version 4.0.8 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID collection . 50710 1 processing . 50710 1 stop_ save_ save_software_2 _Software.Sf_category software _Software.Sf_framecode software_2 _Software.Entry_ID 50710 _Software.ID 2 _Software.Type . _Software.Name NMRFAM-SPARKY _Software.Version 3.13 _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'chemical shift assignment' . 50710 2 'peak picking' . 50710 2 stop_ save_ save_software_3 _Software.Sf_category software _Software.Sf_framecode software_3 _Software.Entry_ID 50710 _Software.ID 3 _Software.Type . _Software.Name CYANA _Software.Version . _Software.DOI . _Software.Details . loop_ _Task.Task _Task.Software_module _Task.Entry_ID _Task.Software_ID 'structure solution' . 50710 3 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_1 _NMR_spectrometer.Entry_ID 50710 _NMR_spectrometer.ID 1 _NMR_spectrometer.Name Sancho _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model 'AVANCE NEO' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ ############################# # NMR applied experiments # ############################# save_experiment_list_1 _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list_1 _Experiment_list.Entry_ID 50710 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NUS_flag _Experiment.Interleaved_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Details _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '1D 1H' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 2 '2D 1H-1H TOCSY' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 3 '2D 1H-1H COSY' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 4 '2D 1H-1H NOESY' no . . . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 5 '1D 1H' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 2 $sample_conditions_2 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 6 '2D 1H-1H COSY' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 2 $sample_conditions_2 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 7 '2D 1H-1H TOCSY' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 2 $sample_conditions_2 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 8 '2D 1H-1H NOESY' no . . . . . . . . . . . . 2 $sample_2 isotropic . . 2 $sample_conditions_2 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 9 '2D 1H-13C HSQC aliphatic' no . . . . . . . . . . . . 3 $sample_3 isotropic . . 3 $sample_conditions_3 . . . 1 $NMR_spectrometer_1 . . . . . . . . . . . . . . . . . 50710 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chem_shift_reference_1 _Chem_shift_reference.Entry_ID 50710 _Chem_shift_reference.ID 1 _Chem_shift_reference.Name 'Reference Set 1' _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.251449530 . . . . . 50710 1 H 1 DSS 'methyl protons' . . . . ppm 0.00 internal direct 1.000000000 . . . . . 50710 1 N 15 DSS 'methyl protons' . . . . ppm 0.00 na indirect 0.101329118 . . . . . 50710 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_1 _Assigned_chem_shift_list.Entry_ID 50710 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Name 'RGG FUS pH 3' _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err 0.01 _Assigned_chem_shift_list.Chem_shift_13C_err 0.1 _Assigned_chem_shift_list.Chem_shift_15N_err 0.1 _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 2 '2D 1H-1H TOCSY' . . . 50710 1 3 '2D 1H-1H COSY' . . . 50710 1 4 '2D 1H-1H NOESY' . . . 50710 1 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 2 $software_2 . . 50710 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 ARG HA H 1 4.01 0.01 . 1 . . . . . 1 ARG HA . 50710 1 2 . 1 . 1 1 1 ARG HB2 H 1 1.92 0.01 . 2 . . . . . 1 ARG HB2 . 50710 1 3 . 1 . 1 1 1 ARG HB3 H 1 1.70 0.01 . 2 . . . . . 1 ARG HB3 . 50710 1 4 . 1 . 1 1 1 ARG HG2 H 1 1.69 0.01 . 1 . . . . . 1 ARG HG2 . 50710 1 5 . 1 . 1 1 1 ARG HG3 H 1 1.69 0.01 . 1 . . . . . 1 ARG HG3 . 50710 1 6 . 1 . 1 1 1 ARG HD2 H 1 3.23 0.01 . 1 . . . . . 1 ARG HD2 . 50710 1 7 . 1 . 1 1 1 ARG HD3 H 1 3.23 0.01 . 1 . . . . . 1 ARG HD3 . 50710 1 8 . 1 . 1 1 1 ARG HE H 1 7.29 0.01 . 1 . . . . . 1 ARG HE . 50710 1 9 . 1 . 1 2 2 GLY H H 1 8.99 0.01 . 1 . . . . . 2 GLY H . 50710 1 10 . 1 . 1 2 2 GLY HA2 H 1 4.06 0.01 . 1 . . . . . 2 GLY HA2 . 50710 1 11 . 1 . 1 2 2 GLY HA3 H 1 4.06 0.01 . 1 . . . . . 2 GLY HA3 . 50710 1 12 . 1 . 1 3 3 GLY H H 1 8.51 0.01 . 1 . . . . . 3 GLY H . 50710 1 13 . 1 . 1 3 3 GLY HA2 H 1 4.00 0.01 . 1 . . . . . 3 GLY HA2 . 50710 1 14 . 1 . 1 3 3 GLY HA3 H 1 4.00 0.01 . 1 . . . . . 3 GLY HA3 . 50710 1 15 . 1 . 1 4 4 ARG H H 1 8.50 0.01 . 1 . . . . . 4 ARG H . 50710 1 16 . 1 . 1 4 4 ARG HA H 1 4.36 0.01 . 1 . . . . . 4 ARG HA . 50710 1 17 . 1 . 1 4 4 ARG HB2 H 1 1.89 0.01 . 2 . . . . . 4 ARG HB2 . 50710 1 18 . 1 . 1 4 4 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 4 ARG HB3 . 50710 1 19 . 1 . 1 4 4 ARG HG2 H 1 1.64 0.01 . 1 . . . . . 4 ARG HG2 . 50710 1 20 . 1 . 1 4 4 ARG HG3 H 1 1.64 0.01 . 1 . . . . . 4 ARG HG3 . 50710 1 21 . 1 . 1 4 4 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 4 ARG HD2 . 50710 1 22 . 1 . 1 4 4 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 4 ARG HD3 . 50710 1 23 . 1 . 1 4 4 ARG HE H 1 7.25 0.01 . 1 . . . . . 4 ARG HE . 50710 1 24 . 1 . 1 6 6 GLY H H 1 8.38 0.01 . 1 . . . . . 6 GLY H . 50710 1 25 . 1 . 1 6 6 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 6 GLY HA2 . 50710 1 26 . 1 . 1 6 6 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 6 GLY HA3 . 50710 1 27 . 1 . 1 7 7 TYR H H 1 8.26 0.01 . 1 . . . . . 7 TYR H . 50710 1 28 . 1 . 1 7 7 TYR HA H 1 4.52 0.01 . 1 . . . . . 7 TYR HA . 50710 1 29 . 1 . 1 7 7 TYR HB2 H 1 2.99 0.01 . 1 . . . . . 7 TYR HB2 . 50710 1 30 . 1 . 1 7 7 TYR HB3 H 1 2.99 0.01 . 1 . . . . . 7 TYR HB3 . 50710 1 31 . 1 . 1 7 7 TYR HD1 H 1 7.10 0.01 . 3 . . . . . 7 TYR HD1 . 50710 1 32 . 1 . 1 7 7 TYR HD2 H 1 7.10 0.01 . 3 . . . . . 7 TYR HD2 . 50710 1 33 . 1 . 1 7 7 TYR HE1 H 1 6.81 0.01 . 3 . . . . . 7 TYR HE1 . 50710 1 34 . 1 . 1 7 7 TYR HE2 H 1 6.81 0.01 . 3 . . . . . 7 TYR HE2 . 50710 1 35 . 1 . 1 8 8 ASP H H 1 8.58 0.01 . 1 . . . . . 8 ASP H . 50710 1 36 . 1 . 1 8 8 ASP HA H 1 4.64 0.01 . 1 . . . . . 8 ASP HA . 50710 1 37 . 1 . 1 8 8 ASP HB2 H 1 2.86 0.01 . 2 . . . . . 8 ASP HB2 . 50710 1 38 . 1 . 1 8 8 ASP HB3 H 1 2.70 0.01 . 2 . . . . . 8 ASP HB3 . 50710 1 39 . 1 . 1 9 9 ARG H H 1 8.39 0.01 . 1 . . . . . 9 ARG H . 50710 1 40 . 1 . 1 9 9 ARG HA H 1 4.26 0.01 . 1 . . . . . 9 ARG HA . 50710 1 41 . 1 . 1 9 9 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 9 ARG HB2 . 50710 1 42 . 1 . 1 9 9 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 9 ARG HB3 . 50710 1 43 . 1 . 1 9 9 ARG HG2 H 1 1.64 0.01 . 1 . . . . . 9 ARG HG2 . 50710 1 44 . 1 . 1 9 9 ARG HG3 H 1 1.64 0.01 . 1 . . . . . 9 ARG HG3 . 50710 1 45 . 1 . 1 9 9 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 9 ARG HD2 . 50710 1 46 . 1 . 1 9 9 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 9 ARG HD3 . 50710 1 47 . 1 . 1 9 9 ARG HE H 1 7.25 0.01 . 1 . . . . . 9 ARG HE . 50710 1 48 . 1 . 1 12 12 TYR H H 1 8.32 0.01 . 1 . . . . . 12 TYR H . 50710 1 49 . 1 . 1 12 12 TYR HA H 1 4.51 0.01 . 1 . . . . . 12 TYR HA . 50710 1 50 . 1 . 1 12 12 TYR HB2 H 1 2.95 0.01 . 1 . . . . . 12 TYR HB2 . 50710 1 51 . 1 . 1 12 12 TYR HB3 H 1 2.95 0.01 . 1 . . . . . 12 TYR HB3 . 50710 1 52 . 1 . 1 12 12 TYR HD1 H 1 7.10 0.01 . 3 . . . . . 12 TYR HD1 . 50710 1 53 . 1 . 1 12 12 TYR HD2 H 1 7.10 0.01 . 3 . . . . . 12 TYR HD2 . 50710 1 54 . 1 . 1 12 12 TYR HE1 H 1 6.81 0.01 . 3 . . . . . 12 TYR HE1 . 50710 1 55 . 1 . 1 12 12 TYR HE2 H 1 6.81 0.01 . 3 . . . . . 12 TYR HE2 . 50710 1 56 . 1 . 1 13 13 ARG H H 1 8.55 0.01 . 1 . . . . . 13 ARG H . 50710 1 57 . 1 . 1 13 13 ARG HA H 1 4.25 0.01 . 1 . . . . . 13 ARG HA . 50710 1 58 . 1 . 1 13 13 ARG HB2 H 1 1.72 0.01 . 2 . . . . . 13 ARG HB2 . 50710 1 59 . 1 . 1 13 13 ARG HB3 H 1 1.64 0.01 . 2 . . . . . 13 ARG HB3 . 50710 1 60 . 1 . 1 13 13 ARG HG2 H 1 1.51 0.01 . 1 . . . . . 13 ARG HG2 . 50710 1 61 . 1 . 1 13 13 ARG HG3 H 1 1.51 0.01 . 1 . . . . . 13 ARG HG3 . 50710 1 62 . 1 . 1 13 13 ARG HD2 H 1 3.11 0.01 . 1 . . . . . 13 ARG HD2 . 50710 1 63 . 1 . 1 13 13 ARG HD3 H 1 3.11 0.01 . 1 . . . . . 13 ARG HD3 . 50710 1 64 . 1 . 1 13 13 ARG HE H 1 7.19 0.01 . 1 . . . . . 13 ARG HE . 50710 1 65 . 1 . 1 14 14 GLY H H 1 7.64 0.01 . 1 . . . . . 14 GLY H . 50710 1 66 . 1 . 1 14 14 GLY HA2 H 1 3.90 0.01 . 1 . . . . . 14 GLY HA2 . 50710 1 67 . 1 . 1 14 14 GLY HA3 H 1 3.90 0.01 . 1 . . . . . 14 GLY HA3 . 50710 1 68 . 1 . 1 15 15 GLY H H 1 8.37 0.01 . 1 . . . . . 15 GLY H . 50710 1 69 . 1 . 1 15 15 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 15 GLY HA2 . 50710 1 70 . 1 . 1 15 15 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 15 GLY HA3 . 50710 1 71 . 1 . 1 16 16 ARG H H 1 8.58 0.01 . 1 . . . . . 16 ARG H . 50710 1 72 . 1 . 1 16 16 ARG HA H 1 4.32 0.01 . 1 . . . . . 16 ARG HA . 50710 1 73 . 1 . 1 16 16 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 16 ARG HB2 . 50710 1 74 . 1 . 1 16 16 ARG HB3 H 1 1.78 0.01 . 2 . . . . . 16 ARG HB3 . 50710 1 75 . 1 . 1 16 16 ARG HG2 H 1 1.64 0.01 . 1 . . . . . 16 ARG HG2 . 50710 1 76 . 1 . 1 16 16 ARG HG3 H 1 1.64 0.01 . 1 . . . . . 16 ARG HG3 . 50710 1 77 . 1 . 1 16 16 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 16 ARG HD2 . 50710 1 78 . 1 . 1 16 16 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 16 ARG HD3 . 50710 1 79 . 1 . 1 16 16 ARG HE H 1 7.25 0.01 . 1 . . . . . 16 ARG HE . 50710 1 80 . 1 . 1 17 17 GLY H H 1 8.48 0.01 . 1 . . . . . 17 GLY H . 50710 1 81 . 1 . 1 17 17 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 17 GLY HA2 . 50710 1 82 . 1 . 1 17 17 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 17 GLY HA3 . 50710 1 83 . 1 . 1 19 19 ASP H H 1 8.51 0.01 . 1 . . . . . 19 ASP H . 50710 1 84 . 1 . 1 19 19 ASP HA H 1 4.70 0.01 . 1 . . . . . 19 ASP HA . 50710 1 85 . 1 . 1 19 19 ASP HB2 H 1 2.90 0.01 . 2 . . . . . 19 ASP HB2 . 50710 1 86 . 1 . 1 19 19 ASP HB3 H 1 2.82 0.01 . 2 . . . . . 19 ASP HB3 . 50710 1 87 . 1 . 1 20 20 ARG H H 1 8.59 0.01 . 1 . . . . . 20 ARG H . 50710 1 88 . 1 . 1 20 20 ARG HA H 1 4.36 0.01 . 1 . . . . . 20 ARG HA . 50710 1 89 . 1 . 1 20 20 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 20 ARG HB2 . 50710 1 90 . 1 . 1 20 20 ARG HB3 H 1 1.78 0.01 . 2 . . . . . 20 ARG HB3 . 50710 1 91 . 1 . 1 20 20 ARG HG2 H 1 1.66 0.01 . 1 . . . . . 20 ARG HG2 . 50710 1 92 . 1 . 1 20 20 ARG HG3 H 1 1.66 0.01 . 1 . . . . . 20 ARG HG3 . 50710 1 93 . 1 . 1 20 20 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 20 ARG HD2 . 50710 1 94 . 1 . 1 20 20 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 20 ARG HD3 . 50710 1 95 . 1 . 1 20 20 ARG HE H 1 7.25 0.01 . 1 . . . . . 20 ARG HE . 50710 1 96 . 1 . 1 21 21 GLY H H 1 8.70 0.01 . 1 . . . . . 21 GLY H . 50710 1 97 . 1 . 1 21 21 GLY HA2 H 1 3.95 0.01 . 2 . . . . . 21 GLY HA2 . 50710 1 98 . 1 . 1 21 21 GLY HA3 H 1 3.95 0.01 . 2 . . . . . 21 GLY HA3 . 50710 1 99 . 1 . 1 22 22 ASN H H 1 8.39 0.01 . 1 . . . . . 22 ASN H . 50710 1 100 . 1 . 1 22 22 ASN HA H 1 4.71 0.01 . 1 . . . . . 22 ASN HA . 50710 1 101 . 1 . 1 22 22 ASN HB2 H 1 2.79 0.01 . 2 . . . . . 22 ASN HB2 . 50710 1 102 . 1 . 1 22 22 ASN HB3 H 1 2.69 0.01 . 2 . . . . . 22 ASN HB3 . 50710 1 103 . 1 . 1 22 22 ASN HD21 H 1 7.67 0.01 . 2 . . . . . 22 ASN HD21 . 50710 1 104 . 1 . 1 22 22 ASN HD22 H 1 7.01 0.01 . 2 . . . . . 22 ASN HD22 . 50710 1 105 . 1 . 1 23 23 TYR H H 1 8.31 0.01 . 1 . . . . . 23 TYR H . 50710 1 106 . 1 . 1 23 23 TYR HA H 1 4.54 0.01 . 1 . . . . . 23 TYR HA . 50710 1 107 . 1 . 1 23 23 TYR HB2 H 1 3.01 0.01 . 1 . . . . . 23 TYR HB2 . 50710 1 108 . 1 . 1 23 23 TYR HB3 H 1 3.01 0.01 . 1 . . . . . 23 TYR HB3 . 50710 1 109 . 1 . 1 23 23 TYR HD1 H 1 7.11 0.01 . 3 . . . . . 23 TYR HD1 . 50710 1 110 . 1 . 1 23 23 TYR HD2 H 1 6.81 0.01 . 3 . . . . . 23 TYR HD2 . 50710 1 111 . 1 . 1 23 23 TYR HE1 H 1 7.25 0.01 . 3 . . . . . 23 TYR HE1 . 50710 1 112 . 1 . 1 23 23 TYR HE2 H 1 7.25 0.01 . 3 . . . . . 23 TYR HE2 . 50710 1 113 . 1 . 1 24 24 ARG H H 1 8.50 0.01 . 1 . . . . . 24 ARG H . 50710 1 114 . 1 . 1 24 24 ARG HA H 1 4.26 0.01 . 1 . . . . . 24 ARG HA . 50710 1 115 . 1 . 1 24 24 ARG HB2 H 1 1.79 0.01 . 2 . . . . . 24 ARG HB2 . 50710 1 116 . 1 . 1 24 24 ARG HB3 H 1 1.65 0.01 . 2 . . . . . 24 ARG HB3 . 50710 1 117 . 1 . 1 24 24 ARG HG2 H 1 1.51 0.01 . 1 . . . . . 24 ARG HG2 . 50710 1 118 . 1 . 1 24 24 ARG HG3 H 1 1.51 0.01 . 1 . . . . . 24 ARG HG3 . 50710 1 119 . 1 . 1 24 24 ARG HD2 H 1 3.15 0.01 . 1 . . . . . 24 ARG HD2 . 50710 1 120 . 1 . 1 24 24 ARG HD3 H 1 3.15 0.01 . 1 . . . . . 24 ARG HD3 . 50710 1 121 . 1 . 1 24 24 ARG HE H 1 7.22 0.01 . 1 . . . . . 24 ARG HE . 50710 1 122 . 1 . 1 25 25 GLY H H 1 7.85 0.01 . 1 . . . . . 25 GLY H . 50710 1 123 . 1 . 1 25 25 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 25 GLY HA2 . 50710 1 124 . 1 . 1 25 25 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 25 GLY HA3 . 50710 1 125 . 1 . 1 26 26 GLY H H 1 8.36 0.01 . 1 . . . . . 26 GLY H . 50710 1 126 . 1 . 1 26 26 GLY HA2 H 1 3.99 0.01 . 1 . . . . . 26 GLY HA2 . 50710 1 127 . 1 . 1 26 26 GLY HA3 H 1 3.99 0.01 . 1 . . . . . 26 GLY HA3 . 50710 1 128 . 1 . 1 27 27 ARG H H 1 8.46 0.01 . 1 . . . . . 27 ARG H . 50710 1 129 . 1 . 1 27 27 ARG HA H 1 4.37 0.01 . 1 . . . . . 27 ARG HA . 50710 1 130 . 1 . 1 27 27 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 27 ARG HB2 . 50710 1 131 . 1 . 1 27 27 ARG HB3 H 1 1.78 0.01 . 2 . . . . . 27 ARG HB3 . 50710 1 132 . 1 . 1 27 27 ARG HG2 H 1 1.64 0.01 . 1 . . . . . 27 ARG HG2 . 50710 1 133 . 1 . 1 27 27 ARG HG3 H 1 1.64 0.01 . 1 . . . . . 27 ARG HG3 . 50710 1 134 . 1 . 1 27 27 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 27 ARG HD2 . 50710 1 135 . 1 . 1 27 27 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 27 ARG HD3 . 50710 1 136 . 1 . 1 27 27 ARG HE H 1 7.25 0.01 . 1 . . . . . 27 ARG HE . 50710 1 137 . 1 . 1 28 28 GLY H H 1 8.67 0.01 . 1 . . . . . 28 GLY H . 50710 1 138 . 1 . 1 28 28 GLY HA2 H 1 3.95 0.01 . 1 . . . . . 28 GLY HA2 . 50710 1 139 . 1 . 1 28 28 GLY HA3 H 1 3.95 0.01 . 1 . . . . . 28 GLY HA3 . 50710 1 140 . 1 . 1 29 29 GLY H H 1 8.35 0.01 . 1 . . . . . 29 GLY H . 50710 1 141 . 1 . 1 29 29 GLY HA2 H 1 3.97 0.01 . 1 . . . . . 29 GLY HA2 . 50710 1 142 . 1 . 1 29 29 GLY HA3 H 1 3.97 0.01 . 1 . . . . . 29 GLY HA3 . 50710 1 143 . 1 . 1 30 30 ASP H H 1 8.28 0.01 . 1 . . . . . 30 ASP H . 50710 1 144 . 1 . 1 30 30 ASP HA H 1 4.66 0.01 . 1 . . . . . 30 ASP HA . 50710 1 145 . 1 . 1 30 30 ASP HB2 H 1 2.89 0.01 . 1 . . . . . 30 ASP HB2 . 50710 1 146 . 1 . 1 30 30 ASP HB3 H 1 2.89 0.01 . 1 . . . . . 30 ASP HB3 . 50710 1 stop_ save_ save_assigned_chemical_shifts_2 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_2 _Assigned_chem_shift_list.Entry_ID 50710 _Assigned_chem_shift_list.ID 2 _Assigned_chem_shift_list.Name 'RGG FUS pH 7' _Assigned_chem_shift_list.Sample_condition_list_ID 2 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_2 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err 0.01 _Assigned_chem_shift_list.Chem_shift_13C_err 0.1 _Assigned_chem_shift_list.Chem_shift_15N_err 0.1 _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 6 '2D 1H-1H COSY' . . . 50710 2 7 '2D 1H-1H TOCSY' . . . 50710 2 8 '2D 1H-1H NOESY' . . . 50710 2 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 2 $software_2 . . 50710 2 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 ARG HA H 1 4.00 0.01 . 1 . . . . . 1 ARG HA . 50710 2 2 . 1 . 1 1 1 ARG HB2 H 1 1.84 0.01 . 2 . . . . . 1 ARG HB2 . 50710 2 3 . 1 . 1 1 1 ARG HB3 H 1 1.64 0.01 . 2 . . . . . 1 ARG HB3 . 50710 2 4 . 1 . 1 1 1 ARG HG2 H 1 1.68 0.01 . 1 . . . . . 1 ARG HG2 . 50710 2 5 . 1 . 1 1 1 ARG HG3 H 1 1.68 0.01 . 1 . . . . . 1 ARG HG3 . 50710 2 6 . 1 . 1 1 1 ARG HD2 H 1 3.19 0.01 . 1 . . . . . 1 ARG HD2 . 50710 2 7 . 1 . 1 1 1 ARG HD3 H 1 3.19 0.01 . 1 . . . . . 1 ARG HD3 . 50710 2 8 . 1 . 1 1 1 ARG HE H 1 7.25 0.01 . 1 . . . . . 1 ARG HE . 50710 2 9 . 1 . 1 4 4 ARG H H 1 8.49 0.01 . 1 . . . . . 4 ARG H . 50710 2 10 . 1 . 1 4 4 ARG HA H 1 4.36 0.01 . 1 . . . . . 4 ARG HA . 50710 2 11 . 1 . 1 4 4 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 4 ARG HB2 . 50710 2 12 . 1 . 1 4 4 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 4 ARG HB3 . 50710 2 13 . 1 . 1 4 4 ARG HG2 H 1 1.63 0.01 . 1 . . . . . 4 ARG HG2 . 50710 2 14 . 1 . 1 4 4 ARG HG3 H 1 1.63 0.01 . 1 . . . . . 4 ARG HG3 . 50710 2 15 . 1 . 1 4 4 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 4 ARG HD2 . 50710 2 16 . 1 . 1 4 4 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 4 ARG HD3 . 50710 2 17 . 1 . 1 4 4 ARG HE H 1 7.29 0.01 . 1 . . . . . 4 ARG HE . 50710 2 18 . 1 . 1 5 5 GLY H H 1 8.70 0.01 . 1 . . . . . 5 GLY H . 50710 2 19 . 1 . 1 5 5 GLY HA2 H 1 3.97 0.01 . 1 . . . . . 5 GLY HA2 . 50710 2 20 . 1 . 1 5 5 GLY HA3 H 1 3.97 0.01 . 1 . . . . . 5 GLY HA3 . 50710 2 21 . 1 . 1 6 6 GLY H H 1 8.25 0.01 . 1 . . . . . 6 GLY H . 50710 2 22 . 1 . 1 6 6 GLY HA2 H 1 3.89 0.01 . 1 . . . . . 6 GLY HA2 . 50710 2 23 . 1 . 1 6 6 GLY HA3 H 1 3.89 0.01 . 1 . . . . . 6 GLY HA3 . 50710 2 24 . 1 . 1 7 7 TYR H H 1 8.22 0.01 . 1 . . . . . 7 TYR H . 50710 2 25 . 1 . 1 7 7 TYR HA H 1 4.53 0.01 . 1 . . . . . 7 TYR HA . 50710 2 26 . 1 . 1 7 7 TYR HB2 H 1 3.04 0.01 . 2 . . . . . 7 TYR HB2 . 50710 2 27 . 1 . 1 7 7 TYR HB3 H 1 2.92 0.01 . 2 . . . . . 7 TYR HB3 . 50710 2 28 . 1 . 1 7 7 TYR HD1 H 1 7.08 0.01 . 3 . . . . . 7 TYR HD1 . 50710 2 29 . 1 . 1 7 7 TYR HD2 H 1 7.08 0.01 . 3 . . . . . 7 TYR HD2 . 50710 2 30 . 1 . 1 7 7 TYR HE1 H 1 6.80 0.01 . 3 . . . . . 7 TYR HE1 . 50710 2 31 . 1 . 1 7 7 TYR HE2 H 1 6.80 0.01 . 3 . . . . . 7 TYR HE2 . 50710 2 32 . 1 . 1 8 8 ASP H H 1 8.49 0.01 . 1 . . . . . 8 ASP H . 50710 2 33 . 1 . 1 8 8 ASP HA H 1 4.58 0.01 . 1 . . . . . 8 ASP HA . 50710 2 34 . 1 . 1 8 8 ASP HB2 H 1 2.69 0.01 . 2 . . . . . 8 ASP HB2 . 50710 2 35 . 1 . 1 8 8 ASP HB3 H 1 2.62 0.01 . 2 . . . . . 8 ASP HB3 . 50710 2 36 . 1 . 1 9 9 ARG H H 1 8.45 0.01 . 1 . . . . . 9 ARG H . 50710 2 37 . 1 . 1 9 9 ARG HA H 1 4.26 0.01 . 1 . . . . . 9 ARG HA . 50710 2 38 . 1 . 1 9 9 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 9 ARG HB2 . 50710 2 39 . 1 . 1 9 9 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 9 ARG HB3 . 50710 2 40 . 1 . 1 9 9 ARG HG2 H 1 1.63 0.01 . 1 . . . . . 9 ARG HG2 . 50710 2 41 . 1 . 1 9 9 ARG HG3 H 1 1.63 0.01 . 1 . . . . . 9 ARG HG3 . 50710 2 42 . 1 . 1 9 9 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 9 ARG HD2 . 50710 2 43 . 1 . 1 9 9 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 9 ARG HD3 . 50710 2 44 . 1 . 1 9 9 ARG HE H 1 7.29 0.01 . 1 . . . . . 9 ARG HE . 50710 2 45 . 1 . 1 10 10 GLY H H 1 8.61 0.01 . 1 . . . . . 10 GLY H . 50710 2 46 . 1 . 1 10 10 GLY HA2 H 1 3.95 0.01 . 1 . . . . . 10 GLY HA2 . 50710 2 47 . 1 . 1 10 10 GLY HA3 H 1 3.95 0.01 . 1 . . . . . 10 GLY HA3 . 50710 2 48 . 1 . 1 11 11 GLY H H 1 8.30 0.01 . 1 . . . . . 11 GLY H . 50710 2 49 . 1 . 1 11 11 GLY HA2 H 1 3.92 0.01 . 1 . . . . . 11 GLY HA2 . 50710 2 50 . 1 . 1 11 11 GLY HA3 H 1 3.92 0.01 . 1 . . . . . 11 GLY HA3 . 50710 2 51 . 1 . 1 12 12 TYR H H 1 8.33 0.01 . 1 . . . . . 12 TYR H . 50710 2 52 . 1 . 1 12 12 TYR HA H 1 4.49 0.01 . 1 . . . . . 12 TYR HA . 50710 2 53 . 1 . 1 12 12 TYR HB2 H 1 2.95 0.01 . 1 . . . . . 12 TYR HB2 . 50710 2 54 . 1 . 1 12 12 TYR HB3 H 1 2.95 0.01 . 1 . . . . . 12 TYR HB3 . 50710 2 55 . 1 . 1 12 12 TYR HD1 H 1 7.10 0.01 . 3 . . . . . 12 TYR HD1 . 50710 2 56 . 1 . 1 12 12 TYR HD2 H 1 7.10 0.01 . 3 . . . . . 12 TYR HD2 . 50710 2 57 . 1 . 1 12 12 TYR HE1 H 1 6.80 0.01 . 3 . . . . . 12 TYR HE1 . 50710 2 58 . 1 . 1 12 12 TYR HE2 H 1 6.80 0.01 . 3 . . . . . 12 TYR HE2 . 50710 2 59 . 1 . 1 13 13 ARG H H 1 8.57 0.01 . 1 . . . . . 13 ARG H . 50710 2 60 . 1 . 1 13 13 ARG HA H 1 4.24 0.01 . 1 . . . . . 13 ARG HA . 50710 2 61 . 1 . 1 13 13 ARG HB2 H 1 1.74 0.01 . 2 . . . . . 13 ARG HB2 . 50710 2 62 . 1 . 1 13 13 ARG HB3 H 1 1.62 0.01 . 2 . . . . . 13 ARG HB3 . 50710 2 63 . 1 . 1 13 13 ARG HG2 H 1 1.62 0.01 . 1 . . . . . 13 ARG HG2 . 50710 2 64 . 1 . 1 13 13 ARG HG3 H 1 1.62 0.01 . 1 . . . . . 13 ARG HG3 . 50710 2 65 . 1 . 1 13 13 ARG HD2 H 1 3.10 0.01 . 1 . . . . . 13 ARG HD2 . 50710 2 66 . 1 . 1 13 13 ARG HD3 H 1 3.10 0.01 . 1 . . . . . 13 ARG HD3 . 50710 2 67 . 1 . 1 14 14 GLY H H 1 7.68 0.01 . 1 . . . . . 14 GLY H . 50710 2 68 . 1 . 1 14 14 GLY HA2 H 1 3.89 0.01 . 1 . . . . . 14 GLY HA2 . 50710 2 69 . 1 . 1 14 14 GLY HA3 H 1 3.89 0.01 . 1 . . . . . 14 GLY HA3 . 50710 2 70 . 1 . 1 15 15 GLY H H 1 8.40 0.01 . 1 . . . . . 15 GLY H . 50710 2 71 . 1 . 1 15 15 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 15 GLY HA2 . 50710 2 72 . 1 . 1 15 15 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 15 GLY HA3 . 50710 2 73 . 1 . 1 16 16 ARG H H 1 8.57 0.01 . 1 . . . . . 16 ARG H . 50710 2 74 . 1 . 1 16 16 ARG HA H 1 4.30 0.01 . 1 . . . . . 16 ARG HA . 50710 2 75 . 1 . 1 16 16 ARG HB2 H 1 1.93 0.01 . 2 . . . . . 16 ARG HB2 . 50710 2 76 . 1 . 1 16 16 ARG HB3 H 1 1.78 0.01 . 2 . . . . . 16 ARG HB3 . 50710 2 77 . 1 . 1 16 16 ARG HG2 H 1 1.66 0.01 . 1 . . . . . 16 ARG HG2 . 50710 2 78 . 1 . 1 16 16 ARG HG3 H 1 1.66 0.01 . 1 . . . . . 16 ARG HG3 . 50710 2 79 . 1 . 1 16 16 ARG HD2 H 1 3.10 0.01 . 1 . . . . . 16 ARG HD2 . 50710 2 80 . 1 . 1 16 16 ARG HD3 H 1 3.10 0.01 . 1 . . . . . 16 ARG HD3 . 50710 2 81 . 1 . 1 16 16 ARG HE H 1 7.24 0.01 . 1 . . . . . 16 ARG HE . 50710 2 82 . 1 . 1 19 19 ASP H H 1 8.40 0.01 . 1 . . . . . 19 ASP H . 50710 2 83 . 1 . 1 19 19 ASP HA H 1 4.60 0.01 . 1 . . . . . 19 ASP HA . 50710 2 84 . 1 . 1 19 19 ASP HB2 H 1 2.77 0.01 . 2 . . . . . 19 ASP HB2 . 50710 2 85 . 1 . 1 19 19 ASP HB3 H 1 2.66 0.01 . 2 . . . . . 19 ASP HB3 . 50710 2 86 . 1 . 1 20 20 ARG H H 1 8.57 0.01 . 1 . . . . . 20 ARG H . 50710 2 87 . 1 . 1 20 20 ARG HA H 1 4.31 0.01 . 1 . . . . . 20 ARG HA . 50710 2 88 . 1 . 1 20 20 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 20 ARG HB2 . 50710 2 89 . 1 . 1 20 20 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 20 ARG HB3 . 50710 2 90 . 1 . 1 20 20 ARG HG2 H 1 1.63 0.01 . 1 . . . . . 20 ARG HG2 . 50710 2 91 . 1 . 1 20 20 ARG HG3 H 1 1.63 0.01 . 1 . . . . . 20 ARG HG3 . 50710 2 92 . 1 . 1 20 20 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 20 ARG HD2 . 50710 2 93 . 1 . 1 20 20 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 20 ARG HD3 . 50710 2 94 . 1 . 1 20 20 ARG HE H 1 7.29 0.01 . 1 . . . . . 20 ARG HE . 50710 2 95 . 1 . 1 21 21 GLY H H 1 8.54 0.01 . 1 . . . . . 21 GLY H . 50710 2 96 . 1 . 1 21 21 GLY HA2 H 1 3.90 0.01 . 1 . . . . . 21 GLY HA2 . 50710 2 97 . 1 . 1 21 21 GLY HA3 H 1 3.90 0.01 . 1 . . . . . 21 GLY HA3 . 50710 2 98 . 1 . 1 22 22 ASN H H 1 8.38 0.01 . 1 . . . . . 22 ASN H . 50710 2 99 . 1 . 1 22 22 ASN HA H 1 4.71 0.01 . 1 . . . . . 22 ASN HA . 50710 2 100 . 1 . 1 22 22 ASN HB2 H 1 2.77 0.01 . 2 . . . . . 22 ASN HB2 . 50710 2 101 . 1 . 1 22 22 ASN HB3 H 1 2.68 0.01 . 2 . . . . . 22 ASN HB3 . 50710 2 102 . 1 . 1 22 22 ASN HD21 H 1 7.70 0.01 . 2 . . . . . 22 ASN HD21 . 50710 2 103 . 1 . 1 22 22 ASN HD22 H 1 7.00 0.01 . 2 . . . . . 22 ASN HD22 . 50710 2 104 . 1 . 1 23 23 TYR H H 1 8.32 0.01 . 1 . . . . . 23 TYR H . 50710 2 105 . 1 . 1 23 23 TYR HA H 1 4.52 0.01 . 1 . . . . . 23 TYR HA . 50710 2 106 . 1 . 1 23 23 TYR HB2 H 1 3.00 0.01 . 1 . . . . . 23 TYR HB2 . 50710 2 107 . 1 . 1 23 23 TYR HB3 H 1 3.00 0.01 . 1 . . . . . 23 TYR HB3 . 50710 2 108 . 1 . 1 23 23 TYR HD1 H 1 7.10 0.01 . 3 . . . . . 23 TYR HD1 . 50710 2 109 . 1 . 1 23 23 TYR HD2 H 1 7.10 0.01 . 3 . . . . . 23 TYR HD2 . 50710 2 110 . 1 . 1 23 23 TYR HE1 H 1 6.80 0.01 . 3 . . . . . 23 TYR HE1 . 50710 2 111 . 1 . 1 23 23 TYR HE2 H 1 6.80 0.01 . 3 . . . . . 23 TYR HE2 . 50710 2 112 . 1 . 1 24 24 ARG H H 1 8.51 0.01 . 1 . . . . . 24 ARG H . 50710 2 113 . 1 . 1 24 24 ARG HA H 1 4.26 0.01 . 1 . . . . . 24 ARG HA . 50710 2 114 . 1 . 1 24 24 ARG HB2 H 1 1.70 0.01 . 2 . . . . . 24 ARG HB2 . 50710 2 115 . 1 . 1 24 24 ARG HB3 H 1 1.67 0.01 . 2 . . . . . 24 ARG HB3 . 50710 2 116 . 1 . 1 24 24 ARG HG2 H 1 1.51 0.01 . 2 . . . . . 24 ARG HG2 . 50710 2 117 . 1 . 1 24 24 ARG HG3 H 1 1.51 0.01 . 2 . . . . . 24 ARG HG3 . 50710 2 118 . 1 . 1 24 24 ARG HD2 H 1 3.14 0.01 . 2 . . . . . 24 ARG HD2 . 50710 2 119 . 1 . 1 24 24 ARG HD3 H 1 3.14 0.01 . 2 . . . . . 24 ARG HD3 . 50710 2 120 . 1 . 1 24 24 ARG HE H 1 7.25 0.01 . 1 . . . . . 24 ARG HE . 50710 2 121 . 1 . 1 25 25 GLY H H 1 7.91 0.01 . 1 . . . . . 25 GLY H . 50710 2 122 . 1 . 1 25 25 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 25 GLY HA2 . 50710 2 123 . 1 . 1 25 25 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 25 GLY HA3 . 50710 2 124 . 1 . 1 26 26 GLY H H 1 8.39 0.01 . 1 . . . . . 26 GLY H . 50710 2 125 . 1 . 1 26 26 GLY HA2 H 1 3.98 0.01 . 2 . . . . . 26 GLY HA2 . 50710 2 126 . 1 . 1 26 26 GLY HA3 H 1 3.98 0.01 . 2 . . . . . 26 GLY HA3 . 50710 2 127 . 1 . 1 27 27 ARG H H 1 8.44 0.01 . 1 . . . . . 27 ARG H . 50710 2 128 . 1 . 1 27 27 ARG HA H 1 4.37 0.01 . 1 . . . . . 27 ARG HA . 50710 2 129 . 1 . 1 27 27 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 27 ARG HB2 . 50710 2 130 . 1 . 1 27 27 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 27 ARG HB3 . 50710 2 131 . 1 . 1 27 27 ARG HG2 H 1 1.63 0.01 . 1 . . . . . 27 ARG HG2 . 50710 2 132 . 1 . 1 27 27 ARG HG3 H 1 1.63 0.01 . 1 . . . . . 27 ARG HG3 . 50710 2 133 . 1 . 1 27 27 ARG HD2 H 1 3.18 0.01 . 1 . . . . . 27 ARG HD2 . 50710 2 134 . 1 . 1 27 27 ARG HD3 H 1 3.18 0.01 . 1 . . . . . 27 ARG HD3 . 50710 2 135 . 1 . 1 27 27 ARG HE H 1 7.29 0.01 . 1 . . . . . 27 ARG HE . 50710 2 136 . 1 . 1 28 28 GLY H H 1 8.66 0.01 . 1 . . . . . 28 GLY H . 50710 2 137 . 1 . 1 28 28 GLY HA2 H 1 3.96 0.01 . 1 . . . . . 28 GLY HA2 . 50710 2 138 . 1 . 1 28 28 GLY HA3 H 1 3.96 0.01 . 1 . . . . . 28 GLY HA3 . 50710 2 139 . 1 . 1 29 29 GLY H H 1 8.34 0.01 . 1 . . . . . 29 GLY H . 50710 2 140 . 1 . 1 29 29 GLY HA2 H 1 3.95 0.01 . 1 . . . . . 29 GLY HA2 . 50710 2 141 . 1 . 1 29 29 GLY HA3 H 1 3.95 0.01 . 1 . . . . . 29 GLY HA3 . 50710 2 142 . 1 . 1 30 30 ASP H H 1 8.10 0.01 . 1 . . . . . 30 ASP H . 50710 2 143 . 1 . 1 30 30 ASP HA H 1 4.43 0.01 . 1 . . . . . 30 ASP HA . 50710 2 144 . 1 . 1 30 30 ASP HB2 H 1 2.69 0.01 . 2 . . . . . 30 ASP HB2 . 50710 2 145 . 1 . 1 30 30 ASP HB3 H 1 2.57 0.01 . 2 . . . . . 30 ASP HB3 . 50710 2 stop_ save_ save_assigned_chemical_shifts_3 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_3 _Assigned_chem_shift_list.Entry_ID 50710 _Assigned_chem_shift_list.ID 3 _Assigned_chem_shift_list.Name 'RGG FUS pH 10' _Assigned_chem_shift_list.Sample_condition_list_ID 3 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_3 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chem_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err 0.01 _Assigned_chem_shift_list.Chem_shift_13C_err 0.1 _Assigned_chem_shift_list.Chem_shift_15N_err 0.1 _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 9 '2D 1H-13C HSQC aliphatic' . . . 50710 3 stop_ loop_ _Chem_shift_software.Software_ID _Chem_shift_software.Software_label _Chem_shift_software.Method_ID _Chem_shift_software.Method_label _Chem_shift_software.Entry_ID _Chem_shift_software.Assigned_chem_shift_list_ID 2 $software_2 . . 50710 3 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_assembly_asym_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Ambiguity_set_ID _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 . 1 1 1 ARG HA H 1 4.01 0.01 . 1 . . . . . 1 ARG HA . 50710 3 2 . 1 . 1 1 1 ARG HB2 H 1 1.92 0.01 . 1 . . . . . 1 ARG HB2 . 50710 3 3 . 1 . 1 1 1 ARG HB3 H 1 1.92 0.01 . 1 . . . . . 1 ARG HB3 . 50710 3 4 . 1 . 1 1 1 ARG HG2 H 1 1.66 0.01 . 1 . . . . . 1 ARG HG2 . 50710 3 5 . 1 . 1 1 1 ARG HG3 H 1 1.66 0.01 . 1 . . . . . 1 ARG HG3 . 50710 3 6 . 1 . 1 1 1 ARG HD2 H 1 3.32 0.01 . 1 . . . . . 1 ARG HD2 . 50710 3 7 . 1 . 1 1 1 ARG HD3 H 1 3.32 0.01 . 1 . . . . . 1 ARG HD3 . 50710 3 8 . 1 . 1 1 1 ARG CA C 13 58.5 0.1 . 1 . . . . . 1 ARG CA . 50710 3 9 . 1 . 1 1 1 ARG CB C 13 30.6 0.1 . 1 . . . . . 1 ARG CB . 50710 3 10 . 1 . 1 1 1 ARG CG C 13 27.1 0.1 . 1 . . . . . 1 ARG CG . 50710 3 11 . 1 . 1 1 1 ARG CD C 13 43.1 0.1 . 1 . . . . . 1 ARG CD . 50710 3 12 . 1 . 1 2 2 GLY HA2 H 1 3.99 0.01 . 1 . . . . . 2 GLY HA2 . 50710 3 13 . 1 . 1 2 2 GLY HA3 H 1 3.99 0.01 . 1 . . . . . 2 GLY HA3 . 50710 3 14 . 1 . 1 2 2 GLY CA C 13 45.2 0.2 . 1 . . . . . 2 GLY CA . 50710 3 15 . 1 . 1 3 3 GLY HA2 H 1 3.98 0.01 . 1 . . . . . 3 GLY HA2 . 50710 3 16 . 1 . 1 3 3 GLY HA3 H 1 3.98 0.01 . 1 . . . . . 3 GLY HA3 . 50710 3 17 . 1 . 1 3 3 GLY CA C 13 45.1 0.2 . 1 . . . . . 3 GLY CA . 50710 3 18 . 1 . 1 4 4 ARG HA H 1 4.34 0.01 . 1 . . . . . 4 ARG HA . 50710 3 19 . 1 . 1 4 4 ARG HB2 H 1 1.92 0.01 . 2 . . . . . 4 ARG HB2 . 50710 3 20 . 1 . 1 4 4 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 4 ARG HB3 . 50710 3 21 . 1 . 1 4 4 ARG HG2 H 1 1.64 0.01 . 1 . . . . . 4 ARG HG2 . 50710 3 22 . 1 . 1 4 4 ARG HG3 H 1 1.64 0.01 . 1 . . . . . 4 ARG HG3 . 50710 3 23 . 1 . 1 4 4 ARG HD2 H 1 3.32 0.01 . 1 . . . . . 4 ARG HD2 . 50710 3 24 . 1 . 1 4 4 ARG HD3 H 1 3.32 0.01 . 1 . . . . . 4 ARG HD3 . 50710 3 25 . 1 . 1 4 4 ARG CA C 13 56.2 0.1 . 1 . . . . . 4 ARG CA . 50710 3 26 . 1 . 1 4 4 ARG CB C 13 30.6 0.1 . 1 . . . . . 4 ARG CB . 50710 3 27 . 1 . 1 4 4 ARG CG C 13 27.1 0.1 . 1 . . . . . 4 ARG CG . 50710 3 28 . 1 . 1 4 4 ARG CD C 13 43.1 0.1 . 1 . . . . . 4 ARG CD . 50710 3 29 . 1 . 1 5 5 GLY HA2 H 1 3.98 0.01 . 1 . . . . . 5 GLY HA2 . 50710 3 30 . 1 . 1 5 5 GLY HA3 H 1 3.98 0.01 . 1 . . . . . 5 GLY HA3 . 50710 3 31 . 1 . 1 5 5 GLY CA C 13 45.2 0.2 . 1 . . . . . 5 GLY CA . 50710 3 32 . 1 . 1 6 6 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 6 GLY HA2 . 50710 3 33 . 1 . 1 6 6 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 6 GLY HA3 . 50710 3 34 . 1 . 1 6 6 GLY CA C 13 45.2 0.2 . 1 . . . . . 6 GLY CA . 50710 3 35 . 1 . 1 7 7 TYR HA H 1 4.51 0.01 . 1 . . . . . 7 TYR HA . 50710 3 36 . 1 . 1 7 7 TYR HB2 H 1 3.01 0.01 . 2 . . . . . 7 TYR HB2 . 50710 3 37 . 1 . 1 7 7 TYR HB3 H 1 2.90 0.01 . 2 . . . . . 7 TYR HB3 . 50710 3 38 . 1 . 1 7 7 TYR HD1 H 1 7.02 0.01 . 3 . . . . . 7 TYR HD1 . 50710 3 39 . 1 . 1 7 7 TYR HD2 H 1 7.02 0.01 . 3 . . . . . 7 TYR HD2 . 50710 3 40 . 1 . 1 7 7 TYR HE1 H 1 6.69 0.01 . 3 . . . . . 7 TYR HE1 . 50710 3 41 . 1 . 1 7 7 TYR HE2 H 1 6.69 0.01 . 3 . . . . . 7 TYR HE2 . 50710 3 42 . 1 . 1 7 7 TYR CA C 13 58.2 0.1 . 1 . . . . . 7 TYR CA . 50710 3 43 . 1 . 1 7 7 TYR CB C 13 38.7 0.1 . 1 . . . . . 7 TYR CB . 50710 3 44 . 1 . 1 8 8 ASP HA H 1 4.58 0.01 . 1 . . . . . 8 ASP HA . 50710 3 45 . 1 . 1 8 8 ASP HB2 H 1 2.69 0.01 . 2 . . . . . 8 ASP HB2 . 50710 3 46 . 1 . 1 8 8 ASP HB3 H 1 2.62 0.01 . 2 . . . . . 8 ASP HB3 . 50710 3 47 . 1 . 1 8 8 ASP CA C 13 54.0 0.1 . 1 . . . . . 8 ASP CA . 50710 3 48 . 1 . 1 8 8 ASP CB C 13 41.1 0.1 . 1 . . . . . 8 ASP CB . 50710 3 49 . 1 . 1 9 9 ARG HA H 1 4.26 0.01 . 1 . . . . . 9 ARG HA . 50710 3 50 . 1 . 1 9 9 ARG HB2 H 1 1.94 0.01 . 2 . . . . . 9 ARG HB2 . 50710 3 51 . 1 . 1 9 9 ARG HB3 H 1 1.78 0.01 . 2 . . . . . 9 ARG HB3 . 50710 3 52 . 1 . 1 9 9 ARG HG2 H 1 1.64 0.01 . 1 . . . . . 9 ARG HG2 . 50710 3 53 . 1 . 1 9 9 ARG HG3 H 1 1.64 0.01 . 1 . . . . . 9 ARG HG3 . 50710 3 54 . 1 . 1 9 9 ARG HD2 H 1 3.16 0.01 . 1 . . . . . 9 ARG HD2 . 50710 3 55 . 1 . 1 9 9 ARG HD3 H 1 3.16 0.01 . 1 . . . . . 9 ARG HD3 . 50710 3 56 . 1 . 1 9 9 ARG CA C 13 56.3 0.1 . 1 . . . . . 9 ARG CA . 50710 3 57 . 1 . 1 9 9 ARG CB C 13 30.4 0.1 . 1 . . . . . 9 ARG CB . 50710 3 58 . 1 . 1 9 9 ARG CG C 13 27.1 0.1 . 1 . . . . . 9 ARG CG . 50710 3 59 . 1 . 1 9 9 ARG CD C 13 43.1 0.1 . 1 . . . . . 9 ARG CD . 50710 3 60 . 1 . 1 10 10 GLY HA2 H 1 3.95 0.01 . 1 . . . . . 10 GLY HA2 . 50710 3 61 . 1 . 1 10 10 GLY HA3 H 1 3.95 0.01 . 1 . . . . . 10 GLY HA3 . 50710 3 62 . 1 . 1 10 10 GLY CA C 13 45.2 0.2 . 1 . . . . . 10 GLY CA . 50710 3 63 . 1 . 1 11 11 GLY HA2 H 1 3.93 0.01 . 1 . . . . . 11 GLY HA2 . 50710 3 64 . 1 . 1 11 11 GLY HA3 H 1 3.93 0.01 . 1 . . . . . 11 GLY HA3 . 50710 3 65 . 1 . 1 11 11 GLY CA C 13 45.2 0.2 . 1 . . . . . 11 GLY CA . 50710 3 66 . 1 . 1 12 12 TYR HA H 1 4.49 0.01 . 1 . . . . . 12 TYR HA . 50710 3 67 . 1 . 1 12 12 TYR HB2 H 1 2.99 0.01 . 2 . . . . . 12 TYR HB2 . 50710 3 68 . 1 . 1 12 12 TYR HB3 H 1 2.91 0.01 . 2 . . . . . 12 TYR HB3 . 50710 3 69 . 1 . 1 12 12 TYR HD1 H 1 7.04 0.01 . 3 . . . . . 12 TYR HD1 . 50710 3 70 . 1 . 1 12 12 TYR HD2 H 1 7.04 0.01 . 3 . . . . . 12 TYR HD2 . 50710 3 71 . 1 . 1 12 12 TYR HE1 H 1 6.69 0.01 . 3 . . . . . 12 TYR HE1 . 50710 3 72 . 1 . 1 12 12 TYR HE2 H 1 6.69 0.01 . 3 . . . . . 12 TYR HE2 . 50710 3 73 . 1 . 1 12 12 TYR CA C 13 58.4 0.1 . 1 . . . . . 12 TYR CA . 50710 3 74 . 1 . 1 12 12 TYR CB C 13 38.8 0.1 . 1 . . . . . 12 TYR CB . 50710 3 75 . 1 . 1 13 13 ARG HA H 1 4.23 0.01 . 1 . . . . . 13 ARG HA . 50710 3 76 . 1 . 1 13 13 ARG HB2 H 1 1.83 0.01 . 2 . . . . . 13 ARG HB2 . 50710 3 77 . 1 . 1 13 13 ARG HB3 H 1 1.62 0.01 . 2 . . . . . 13 ARG HB3 . 50710 3 78 . 1 . 1 13 13 ARG HG2 H 1 1.48 0.01 . 1 . . . . . 13 ARG HG2 . 50710 3 79 . 1 . 1 13 13 ARG HG3 H 1 1.48 0.01 . 1 . . . . . 13 ARG HG3 . 50710 3 80 . 1 . 1 13 13 ARG HD2 H 1 3.10 0.01 . 1 . . . . . 13 ARG HD2 . 50710 3 81 . 1 . 1 13 13 ARG HD3 H 1 3.10 0.01 . 1 . . . . . 13 ARG HD3 . 50710 3 82 . 1 . 1 13 13 ARG CA C 13 56.0 0.1 . 1 . . . . . 13 ARG CA . 50710 3 83 . 1 . 1 13 13 ARG CB C 13 30.4 0.1 . 1 . . . . . 13 ARG CB . 50710 3 84 . 1 . 1 13 13 ARG CG C 13 27.0 0.1 . 1 . . . . . 13 ARG CG . 50710 3 85 . 1 . 1 13 13 ARG CD C 13 43.0 0.1 . 1 . . . . . 13 ARG CD . 50710 3 86 . 1 . 1 14 14 GLY HA2 H 1 3.89 0.01 . 1 . . . . . 14 GLY HA2 . 50710 3 87 . 1 . 1 14 14 GLY HA3 H 1 3.89 0.01 . 1 . . . . . 14 GLY HA3 . 50710 3 88 . 1 . 1 14 14 GLY CA C 13 45.3 0.2 . 1 . . . . . 14 GLY CA . 50710 3 89 . 1 . 1 15 15 GLY HA2 H 1 3.91 0.01 . 1 . . . . . 15 GLY HA2 . 50710 3 90 . 1 . 1 15 15 GLY HA3 H 1 3.91 0.01 . 1 . . . . . 15 GLY HA3 . 50710 3 91 . 1 . 1 15 15 GLY CA C 13 45.4 0.2 . 1 . . . . . 15 GLY CA . 50710 3 92 . 1 . 1 16 16 ARG HA H 1 4.29 0.01 . 1 . . . . . 16 ARG HA . 50710 3 93 . 1 . 1 16 16 ARG HB2 H 1 1.93 0.01 . 2 . . . . . 16 ARG HB2 . 50710 3 94 . 1 . 1 16 16 ARG HB3 H 1 1.78 0.01 . 2 . . . . . 16 ARG HB3 . 50710 3 95 . 1 . 1 16 16 ARG HG2 H 1 1.63 0.01 . 1 . . . . . 16 ARG HG2 . 50710 3 96 . 1 . 1 16 16 ARG HG3 H 1 1.63 0.01 . 1 . . . . . 16 ARG HG3 . 50710 3 97 . 1 . 1 16 16 ARG HD2 H 1 3.16 0.01 . 1 . . . . . 16 ARG HD2 . 50710 3 98 . 1 . 1 16 16 ARG HD3 H 1 3.16 0.01 . 1 . . . . . 16 ARG HD3 . 50710 3 99 . 1 . 1 16 16 ARG CA C 13 56.3 0.1 . 1 . . . . . 16 ARG CA . 50710 3 100 . 1 . 1 16 16 ARG CB C 13 30.5 0.1 . 1 . . . . . 16 ARG CB . 50710 3 101 . 1 . 1 16 16 ARG CG C 13 27.1 0.1 . 1 . . . . . 16 ARG CG . 50710 3 102 . 1 . 1 16 16 ARG CD C 13 43.1 0.1 . 1 . . . . . 16 ARG CD . 50710 3 103 . 1 . 1 17 17 GLY HA2 H 1 3.90 0.01 . 1 . . . . . 17 GLY HA2 . 50710 3 104 . 1 . 1 17 17 GLY HA3 H 1 3.90 0.01 . 1 . . . . . 17 GLY HA3 . 50710 3 105 . 1 . 1 17 17 GLY CA C 13 45.2 0.2 . 1 . . . . . 17 GLY CA . 50710 3 106 . 1 . 1 18 18 GLY HA2 H 1 3.94 0.01 . 1 . . . . . 18 GLY HA2 . 50710 3 107 . 1 . 1 18 18 GLY HA3 H 1 3.94 0.01 . 1 . . . . . 18 GLY HA3 . 50710 3 108 . 1 . 1 18 18 GLY CA C 13 45.4 0.2 . 1 . . . . . 18 GLY CA . 50710 3 109 . 1 . 1 19 19 ASP HA H 1 4.59 0.01 . 1 . . . . . 19 ASP HA . 50710 3 110 . 1 . 1 19 19 ASP HB2 H 1 2.71 0.01 . 2 . . . . . 19 ASP HB2 . 50710 3 111 . 1 . 1 19 19 ASP HB3 H 1 2.66 0.01 . 1 . . . . . 19 ASP HB3 . 50710 3 112 . 1 . 1 19 19 ASP CA C 13 54.3 0.1 . 1 . . . . . 19 ASP CA . 50710 3 113 . 1 . 1 19 19 ASP CB C 13 41.1 0.1 . 1 . . . . . 19 ASP CB . 50710 3 114 . 1 . 1 20 20 ARG HA H 1 4.34 0.01 . 1 . . . . . 20 ARG HA . 50710 3 115 . 1 . 1 20 20 ARG HB2 H 1 1.92 0.01 . 2 . . . . . 20 ARG HB2 . 50710 3 116 . 1 . 1 20 20 ARG HB3 H 1 1.77 0.01 . 2 . . . . . 20 ARG HB3 . 50710 3 117 . 1 . 1 20 20 ARG HG2 H 1 1.63 0.01 . 1 . . . . . 20 ARG HG2 . 50710 3 118 . 1 . 1 20 20 ARG HG3 H 1 1.63 0.01 . 1 . . . . . 20 ARG HG3 . 50710 3 119 . 1 . 1 20 20 ARG HD2 H 1 3.17 0.01 . 1 . . . . . 20 ARG HD2 . 50710 3 120 . 1 . 1 20 20 ARG HD3 H 1 3.17 0.01 . 1 . . . . . 20 ARG HD3 . 50710 3 121 . 1 . 1 20 20 ARG CA C 13 56.4 0.1 . 1 . . . . . 20 ARG CA . 50710 3 122 . 1 . 1 20 20 ARG CB C 13 30.5 0.1 . 1 . . . . . 20 ARG CB . 50710 3 123 . 1 . 1 20 20 ARG CG C 13 27.1 0.1 . 1 . . . . . 20 ARG CG . 50710 3 124 . 1 . 1 20 20 ARG CD C 13 43.0 0.1 . 1 . . . . . 20 ARG CD . 50710 3 125 . 1 . 1 21 21 GLY HA2 H 1 3.94 0.01 . 1 . . . . . 21 GLY HA2 . 50710 3 126 . 1 . 1 21 21 GLY HA3 H 1 3.94 0.01 . 1 . . . . . 21 GLY HA3 . 50710 3 127 . 1 . 1 21 21 GLY CA C 13 45.2 0.2 . 1 . . . . . 21 GLY CA . 50710 3 128 . 1 . 1 22 22 ASN HA H 1 4.71 0.01 . 1 . . . . . 22 ASN HA . 50710 3 129 . 1 . 1 22 22 ASN HB2 H 1 2.77 0.01 . 2 . . . . . 22 ASN HB2 . 50710 3 130 . 1 . 1 22 22 ASN HB3 H 1 2.67 0.01 . 2 . . . . . 22 ASN HB3 . 50710 3 131 . 1 . 1 22 22 ASN CA C 13 52.8 0.1 . 1 . . . . . 22 ASN CA . 50710 3 132 . 1 . 1 22 22 ASN CB C 13 38.5 0.1 . 1 . . . . . 22 ASN CB . 50710 3 133 . 1 . 1 23 23 TYR HA H 1 4.50 0.01 . 1 . . . . . 23 TYR HA . 50710 3 134 . 1 . 1 23 23 TYR HB2 H 1 1.85 0.01 . 2 . . . . . 23 TYR HB2 . 50710 3 135 . 1 . 1 23 23 TYR HB3 H 1 1.65 0.01 . 2 . . . . . 23 TYR HB3 . 50710 3 136 . 1 . 1 23 23 TYR HD1 H 1 7.04 0.01 . 3 . . . . . 23 TYR HD1 . 50710 3 137 . 1 . 1 23 23 TYR HD2 H 1 7.04 0.01 . 3 . . . . . 23 TYR HD2 . 50710 3 138 . 1 . 1 23 23 TYR HE1 H 1 6.69 0.01 . 3 . . . . . 23 TYR HE1 . 50710 3 139 . 1 . 1 23 23 TYR HE2 H 1 6.69 0.01 . 3 . . . . . 23 TYR HE2 . 50710 3 140 . 1 . 1 23 23 TYR CA C 13 58.20 0.1 . 1 . . . . . 23 TYR CA . 50710 3 141 . 1 . 1 23 23 TYR CB C 13 38.6 0.1 . 1 . . . . . 23 TYR CB . 50710 3 142 . 1 . 1 24 24 ARG HA H 1 4.25 0.01 . 1 . . . . . 24 ARG HA . 50710 3 143 . 1 . 1 24 24 ARG HB2 H 1 1.85 0.01 . 1 . . . . . 24 ARG HB2 . 50710 3 144 . 1 . 1 24 24 ARG HB3 H 1 1.85 0.01 . 1 . . . . . 24 ARG HB3 . 50710 3 145 . 1 . 1 24 24 ARG HG2 H 1 1.49 0.01 . 1 . . . . . 24 ARG HG2 . 50710 3 146 . 1 . 1 24 24 ARG HG3 H 1 1.49 0.01 . 1 . . . . . 24 ARG HG3 . 50710 3 147 . 1 . 1 24 24 ARG HD2 H 1 3.12 0.01 . 1 . . . . . 24 ARG HD2 . 50710 3 148 . 1 . 1 24 24 ARG HD3 H 1 3.12 0.01 . 1 . . . . . 24 ARG HD3 . 50710 3 149 . 1 . 1 24 24 ARG CA C 13 56.3 0.1 . 1 . . . . . 24 ARG CA . 50710 3 150 . 1 . 1 24 24 ARG CB C 13 30.4 0.1 . 1 . . . . . 24 ARG CB . 50710 3 151 . 1 . 1 24 24 ARG CG C 13 27.0 0.1 . 1 . . . . . 24 ARG CG . 50710 3 152 . 1 . 1 24 24 ARG CD C 13 43.1 0.1 . 1 . . . . . 24 ARG CD . 50710 3 153 . 1 . 1 25 25 GLY HA2 H 1 3.92 0.01 . 1 . . . . . 25 GLY HA2 . 50710 3 154 . 1 . 1 25 25 GLY HA3 H 1 3.92 0.01 . 1 . . . . . 25 GLY HA3 . 50710 3 155 . 1 . 1 25 25 GLY CA C 13 45.3 0.2 . 1 . . . . . 25 GLY CA . 50710 3 156 . 1 . 1 26 26 GLY HA2 H 1 3.99 0.01 . 1 . . . . . 26 GLY HA2 . 50710 3 157 . 1 . 1 26 26 GLY HA3 H 1 3.99 0.01 . 1 . . . . . 26 GLY HA3 . 50710 3 158 . 1 . 1 26 26 GLY CA C 13 45.3 0.2 . 1 . . . . . 26 GLY CA . 50710 3 159 . 1 . 1 27 27 ARG HA H 1 4.37 0.01 . 1 . . . . . 27 ARG HA . 50710 3 160 . 1 . 1 27 27 ARG HB2 H 1 1.91 0.01 . 2 . . . . . 27 ARG HB2 . 50710 3 161 . 1 . 1 27 27 ARG HB3 H 1 1.79 0.01 . 2 . . . . . 27 ARG HB3 . 50710 3 162 . 1 . 1 27 27 ARG HG2 H 1 1.62 0.01 . 1 . . . . . 27 ARG HG2 . 50710 3 163 . 1 . 1 27 27 ARG HG3 H 1 1.62 0.01 . 1 . . . . . 27 ARG HG3 . 50710 3 164 . 1 . 1 27 27 ARG HD2 H 1 3.17 0.01 . 1 . . . . . 27 ARG HD2 . 50710 3 165 . 1 . 1 27 27 ARG HD3 H 1 3.17 0.01 . 1 . . . . . 27 ARG HD3 . 50710 3 166 . 1 . 1 27 27 ARG CA C 13 56.3 0.1 . 1 . . . . . 27 ARG CA . 50710 3 167 . 1 . 1 27 27 ARG CB C 13 30.5 0.1 . 1 . . . . . 27 ARG CB . 50710 3 168 . 1 . 1 27 27 ARG CG C 13 27.1 0.1 . 1 . . . . . 27 ARG CG . 50710 3 169 . 1 . 1 27 27 ARG CD C 13 43.0 0.1 . 1 . . . . . 27 ARG CD . 50710 3 170 . 1 . 1 28 28 GLY HA2 H 1 3.95 0.01 . 1 . . . . . 28 GLY HA2 . 50710 3 171 . 1 . 1 28 28 GLY HA3 H 1 3.95 0.01 . 1 . . . . . 28 GLY HA3 . 50710 3 172 . 1 . 1 28 28 GLY CA C 13 45.3 0.2 . 1 . . . . . 28 GLY CA . 50710 3 173 . 1 . 1 29 29 GLY HA2 H 1 3.95 0.01 . 1 . . . . . 29 GLY HA2 . 50710 3 174 . 1 . 1 29 29 GLY HA3 H 1 3.95 0.01 . 1 . . . . . 29 GLY HA3 . 50710 3 175 . 1 . 1 29 29 GLY CA C 13 45.3 0.2 . 1 . . . . . 29 GLY CA . 50710 3 176 . 1 . 1 30 30 ASP HA H 1 4.43 0.01 . 1 . . . . . 30 ASP HA . 50710 3 177 . 1 . 1 30 30 ASP HB2 H 1 2.70 0.01 . 2 . . . . . 30 ASP HB2 . 50710 3 178 . 1 . 1 30 30 ASP HB3 H 1 2.57 0.01 . 2 . . . . . 30 ASP HB3 . 50710 3 179 . 1 . 1 30 30 ASP CA C 13 55.8 0.1 . 1 . . . . . 30 ASP CA . 50710 3 180 . 1 . 1 30 30 ASP CB C 13 42.0 0.1 . 1 . . . . . 30 ASP CB . 50710 3 stop_ save_