BMRB Entry 5011

Title:
Anabaena apoflavodoxin hydrogen exchange: on the stable exchange core of the alfa/beta(21345) flavodoxin-like family
Deposition date:
2001-05-11
Original release date:
2001-06-14
Authors:
Langdon, Grant; Jimenez, M. Angeles; Genzor, Carlos; Maldonado, Susana; Sancho, Javier; Rico, Manuel
Citation:

Citation: Langdon, Grant; Jimenez, M. Angeles; Genzor, Carlos; Maldonado, Susana; Sancho, Javier; Rico, Manuel. "Anabaena apoflavodoxin hydrogen exchange: on the stable exchange core of the alfa/beta(21345) flavodoxin-like family"  Proteins: Struct. Funct. Genet. 43, 476-488 (2001).

Assembly members:

Assembly members:
Anabaena apoflavodoxin, polymer, 169 residues, 18886 Da.

Natural source:

Natural source:   Common Name: Anabaena PCC7119   Taxonomy ID: 1168   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena PCC7119

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ptrc99a

Data sets:
Data typeCount
1H chemical shifts861
13C chemical shifts23
15N chemical shifts156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apofld monomer1

Entities:

Entity 1, apofld monomer 169 residues - 18886 Da.

1   ALALYSLYSILEGLYLEUPHETYRGLYTHR
2   GLNTHRGLYLYSTHRGLUSERVALALAGLU
3   ILEILEARGASPGLUPHEGLYASNASPVAL
4   VALTHRLEUHISASPVALSERGLNALAGLU
5   VALTHRASPLEUASNASPTYRGLNTYRLEU
6   ILEILEGLYCYSPROTHRTRPASNILEGLY
7   GLULEUGLNSERASPTRPGLUGLYLEUTYR
8   SERGLULEUASPASPVALASPPHEASNGLY
9   LYSLEUVALALATYRPHEGLYTHRGLYASP
10   GLNILEGLYTYRALAASPASNPHEGLNASP
11   ALAILEGLYILELEUGLUGLULYSILESER
12   GLNARGGLYGLYLYSTHRVALGLYTYRTRP
13   SERTHRASPGLYTYRASPPHEASNASPSER
14   LYSALALEUARGASNGLYLYSPHEVALGLY
15   LEUALALEUASPGLUASPASNGLNSERASP
16   LEUTHRASPASPARGILELYSSERTRPVAL
17   ALAGLNLEULYSSERGLUPHEGLYLEU

Samples:

sample_1: Anabaena apoflavodoxin 2.0 mM; sodium phosphate 50 mM; dithiothreitol 2 mM

sample_2: Anabaena apoflavodoxin, [U-15N], 2.0 mM; sodium phosphate 50 mM; dithiothreitol 2 mM

sample_3: Anabaena apoflavodoxin 2.0 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYnot availablenot availablenot available
2D 1H-1H CLEAN-TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

BMRB 1580 16593
PDB
DBJ BAB74104
EMBL CAA32720
GB AAB20462
PIR S04600 S18374
REF NP_486445 WP_010996561
SP P0A3D9 P0A3E0
AlphaFold P0A3D9 P0A3E0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks