BMRB Entry 4328

Title:
1H, 13C, and 15N Chemical Shift Assignments for Human Rad51(1-114)
Deposition date:
1999-04-03
Original release date:
1999-09-13
Authors:
Aihara, Hideki; Ito, Yutaka; Kurumizaka, Hitoshi; Yokoyama, Shigeyuki; Shibata, Takehiko
Citation:

Citation: Aihara, Hideki; Ito, Yutaka; Kurumizaka, Hitoshi; Yokoyama, Shigeyuki; Shibata, Takehiko. "The N-terminal Domain of the Human Rad51 Protein Binds DNA; Structure and a DNA Binding Surface as Revealed by NMR"  J. Mol. Biol. 290, 495-504 (1999).

Assembly members:

Assembly members:
Human Rad51 protein(1-114), polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts332
15N chemical shifts111
1H chemical shifts628

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HsRad51(1-114)1

Entities:

Entity 1, HsRad51(1-114) 114 residues - Formula weight is not available

1   METALAMETGLNMETGLNLEUGLUALAASN
2   ALAASPTHRSERVALGLUGLUGLUSERPHE
3   GLYPROGLNPROILESERARGLEUGLUGLN
4   CYSGLYILEASNALAASNASPVALLYSLYS
5   LEUGLUGLUALAGLYPHEHISTHRVALGLU
6   ALAVALALATYRALAPROLYSLYSGLULEU
7   ILEASNILELYSGLYILESERGLUALALYS
8   ALAASPLYSILELEUALAGLUALAALALYS
9   LEUVALPROMETGLYPHETHRTHRALATHR
10   GLUPHEHISGLNARGARGSERGLUILEILE
11   GLNILETHRTHRGLYSERLYSGLULEUASP
12   LYSLEULEUGLN

Samples:

sample_1: Human Rad51 protein(1-114), [U-95% 13C, U-95% 15N], 1.0 – 2.0 mM; Sodium_Phosphate 20.0 mM; NaCl 100 mM; DTT 2 mM; NaN3 0.02%

sample_conditions_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

ANSIG -

NMR spectrometers:

  • Bruker DRX600 600 MHz
  • Bruker ARX400 400 MHz

Related Database Links:

BMRB 11046
PDB
DBJ BAA02718 BAA02961 BAA02962 BAA03189 BAB27489
EMBL CAA69384 CAG38796
GB AAC28561 AAD49705 AAF61901 AAF69145 AAH27384
PRF 2102359A
REF NP_001003043 NP_001039644 NP_001075493 NP_001102674 NP_001116653
SP O77507 P70099 Q06609 Q08297 Q2KJ94
TPG DAA25435
AlphaFold O77507 P70099 Q06609 Q08297 Q2KJ94

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks