BMRB Entry 4142

Title:
1H Chemical Shift Assignments of the Complex between Palmitate and a Non-Specific Lipid Transfer Protein (ns-LTP1) Isolated from Barley Seeds
Deposition date:
1998-05-18
Original release date:
1999-03-01
Authors:
Lerche, Mathilde; Poulsen, Flemming
Citation:

Citation: Lerche, Mathilde; Poulsen, Flemming. "Assignments of 1H and 13C Resonances in the Complex of Palmitate and a Non-specific Lipid Transfer Protein (ns-LTP) Isolated from Barley Seeds."  J. Biomol. NMR 12, 571-572 (1998).

Assembly members:

Assembly members:
LTP, polymer, 91 residues, 9694 Da.
PLM, non-polymer, 256.424 Da.

Natural source:

Natural source:   Common Name: barley   Taxonomy ID: 4513   Superkingdom: EuKaryota   Kingdom: Viridiplantae   Genus/species: Hordeum vulgare

Experimental source:

Experimental source:   Production method: purified from the natural source

Data typeCount
13C chemical shifts7
1H chemical shifts595

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ns-LTP11
2Palmitic acid2

Entities:

Entity 1, ns-LTP1 91 residues - 9694 Da.

1   LEUASNCYSGLYGLNVALASPSERLYSMET
2   LYSPROCYSLEUTHRTYRVALGLNGLYGLY
3   PROGLYPROSERGLYGLUCYSCYSASNGLY
4   VALARGASPLEUHISASNGLNALAGLNSER
5   SERGLYASPARGGLNTHRVALCYSASNCYS
6   LEULYSGLYILEALAARGGLYILEHISASN
7   LEUASNLEUASNASNALAALASERILEPRO
8   SERLYSCYSASNVALASNVALPROTYRTHR
9   ILESERPROASPILEASPCYSSERARGILE
10   TYR

Entity 2, Palmitic acid - C16 H32 O2 - 256.424 Da.

1   PLM

Samples:

sample_one: LTP 2.1 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 7.2 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

PRONTO v970801, Carlsberg A/S - assignments

NMR spectrometers:

    Related Database Links:

    BMRB 15143
    PDB
    DBJ BAK05317
    EMBL CAA28805 CAA41946 CAA42832 CAP16136
    GB AAA32970
    SP P07597
    AlphaFold P07597