BMRB Entry 4083

Title:
Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C, 15N) NMR Spectroscopy
Deposition date:
1997-12-23
Original release date:
2001-02-17
Authors:
Moy, Frankln; Lowry, David; Matsumura, Philip; Dahlquist, Frederick; Krywko, James; Domaille, Peter
Citation:

Citation: Moy, Frankln; Lowry, David; Matsumura, Philip; Dahlquist, Frederick; Krywko, James; Domaille, Peter. "Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C, 15N) NMR Spectroscopy"  Biochemistry 33, 10731-10742 (1994).

Assembly members:

Assembly members:
E. coli CheY, polymer, 128 residues, 24.305 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts423
15N chemical shifts127
1H chemical shifts886

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CheY1

Entities:

Entity 1, CheY 128 residues - 24.305 Da.

1   ALAASPLYSGLULEULYSPHELEUVALVAL
2   ASPASPPHESERTHRMETARGARGILEVAL
3   ARGASNLEULEULYSGLULEUGLYPHEASN
4   ASNVALGLUGLUALAGLUASPGLYVALASP
5   ALALEUASNLYSLEUGLNALAGLYGLYTYR
6   GLYPHEVALILESERASPTRPASNMETPRO
7   ASNMETASPGLYLEUGLULEULEULYSTHR
8   ILEARGALAASPGLYALAMETSERALALEU
9   PROVALLEUMETVALTHRALAGLUALALYS
10   LYSGLUASNILEILEALAALAALAGLNALA
11   GLYALASERGLYTYRVALVALLYSPROPHE
12   THRALAALATHRLEUGLUGLULYSLEUASN
13   LYSILEPHEGLULYSLEUGLYMET

Samples:

sample_one: E. coli CheY, [U-15N], 1.0 – 4.0 mM; sodium phosphate 100 mM; magnesium chloride4 – 5 mM; sodium azide 0.02%; D2O 10%; H2O 90%

sample_two: E. coli CheY, [U-13C; U-15N], 1.0 – 4.0 mM; sodium phosphate 100 mM; magnesium chloride4 – 5 mM; sodium azide 0.02%; D2O 10%; H2O 90%

sample_three: E. coli CheY, [U-13C; U-15N], 1.0 – 4.0 mM; sodium phosphate 100 mM; magnesium chloride4 – 5 mM; sodium azide 0.02%; D2O 99.8%

sample_conditions: pH: 6.75; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 2950 2951 3440 4472
PDB
DBJ BAA15698 BAB36015 BAG77641 BAI25973 BAI30936
EMBL CAD05667 CAP76371 CAQ32359 CAQ98822 CAR03242
GB AAA23570 AAA23577 AAA27037 AAC74952 AAG56872
PIR AH0745
REF NP_310619 NP_416396 NP_456482 NP_460873 NP_707777
SP P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69
AlphaFold P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks