BMRB Entry 4472
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4472
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Cho, Ho; Lee, Seok-Yong; Yan, Dalai; Pan, Xiaoyu; Parkinson, John; Kustu, Sydney; Wemmer, David; Pelton, Jeffrey. "NMR Structure of Activated CheY" J. Mol. Biol. 297, 543-551 (2000).
Assembly members:
Chemotaxis protein Y, polymer, 129 residues, 14097 Da.
BERYLLIUM DIFLUORIDE, non-polymer, 47.009 Da.
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Chemotaxis protein Y: MADKELKFLVVDDFSTMRRI
VRNLLKELGFNNVEEAEDGV
DALNKLQAGGYGFVISDWNM
PNMDGLELLKTIRADGAMSA
LPVLMVTAEAKKENIIAAAQ
AGASGYVVKPFTAATLEEKL
NKIFEKLGM
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 822 |
| 13C chemical shifts | 405 |
| 15N chemical shifts | 123 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CheY | 1 |
| 2 | beryllofluoride | 2 |
Entities:
Entity 1, CheY 129 residues - 14097 Da.
| 1 | MET | ALA | ASP | LYS | GLU | LEU | LYS | PHE | LEU | VAL | ||||
| 2 | VAL | ASP | ASP | PHE | SER | THR | MET | ARG | ARG | ILE | ||||
| 3 | VAL | ARG | ASN | LEU | LEU | LYS | GLU | LEU | GLY | PHE | ||||
| 4 | ASN | ASN | VAL | GLU | GLU | ALA | GLU | ASP | GLY | VAL | ||||
| 5 | ASP | ALA | LEU | ASN | LYS | LEU | GLN | ALA | GLY | GLY | ||||
| 6 | TYR | GLY | PHE | VAL | ILE | SER | ASP | TRP | ASN | MET | ||||
| 7 | PRO | ASN | MET | ASP | GLY | LEU | GLU | LEU | LEU | LYS | ||||
| 8 | THR | ILE | ARG | ALA | ASP | GLY | ALA | MET | SER | ALA | ||||
| 9 | LEU | PRO | VAL | LEU | MET | VAL | THR | ALA | GLU | ALA | ||||
| 10 | LYS | LYS | GLU | ASN | ILE | ILE | ALA | ALA | ALA | GLN | ||||
| 11 | ALA | GLY | ALA | SER | GLY | TYR | VAL | VAL | LYS | PRO | ||||
| 12 | PHE | THR | ALA | ALA | THR | LEU | GLU | GLU | LYS | LEU | ||||
| 13 | ASN | LYS | ILE | PHE | GLU | LYS | LEU | GLY | MET |
Entity 2, beryllofluoride - Be F2 - 47.009 Da.
| 1 | BF2 |
Samples:
sample_1: Chemotaxis protein Y, [U-99% 13C; U-99% 15N], 1 – 4 mM
sample_2: Chemotaxis protein Y, [U-99% 15N], 1 – 4 mM
sample_3: Chemotaxis protein Y, [U-10% 13C], 1 – 4 mM
Ex-cond_1: pH: 6.7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | Ex-cond_1 |
| CBCACONH | not available | not available | Ex-cond_1 |
| 1H-15N NOESY | not available | not available | Ex-cond_1 |
| 1H-15N TOCSY | not available | not available | Ex-cond_1 |
| HCCH-TOCSY | not available | not available | Ex-cond_1 |
| 4D 1H-13C HMQC-NOESY-HMQC | not available | not available | Ex-cond_1 |
| 4D 1H-15N HMQC-NOESY-HSQC | not available | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 2950 2951 3440 4083 |
| PDB | |
| DBJ | BAA15698 BAB36015 BAG77641 BAI25973 BAI30936 |
| EMBL | CAD05667 CAP76371 CAQ32359 CAQ98822 CAR03242 |
| GB | AAA23570 AAA23577 AAA27037 AAC74952 AAG56872 |
| PIR | AH0745 |
| REF | NP_310619 NP_416396 NP_456482 NP_460873 NP_707777 |
| SP | P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69 |
| AlphaFold | P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks