BMRB Entry 4472

Title:
1H, 13C, and 15N signal assignments for BeFx-activated CheY from E. coli
Deposition date:
1999-12-03
Original release date:
2000-12-15
Authors:
Cho, Ho; Lee, Seok-Yong; Yan, Dalai; Pan, Xiaoyu; Parkinson, John; Kustu, Sydney; Wemmer, David; Pelton, Jeffrey
Citation:

Citation: Cho, Ho; Lee, Seok-Yong; Yan, Dalai; Pan, Xiaoyu; Parkinson, John; Kustu, Sydney; Wemmer, David; Pelton, Jeffrey. "NMR Structure of Activated CheY"  J. Mol. Biol. 297, 543-551 (2000).

Assembly members:

Assembly members:
Chemotaxis protein Y, polymer, 129 residues, 14097 Da.
BERYLLIUM DIFLUORIDE, non-polymer, 47.009 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts822
13C chemical shifts405
15N chemical shifts123

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CheY1
2beryllofluoride2

Entities:

Entity 1, CheY 129 residues - 14097 Da.

1   METALAASPLYSGLULEULYSPHELEUVAL
2   VALASPASPPHESERTHRMETARGARGILE
3   VALARGASNLEULEULYSGLULEUGLYPHE
4   ASNASNVALGLUGLUALAGLUASPGLYVAL
5   ASPALALEUASNLYSLEUGLNALAGLYGLY
6   TYRGLYPHEVALILESERASPTRPASNMET
7   PROASNMETASPGLYLEUGLULEULEULYS
8   THRILEARGALAASPGLYALAMETSERALA
9   LEUPROVALLEUMETVALTHRALAGLUALA
10   LYSLYSGLUASNILEILEALAALAALAGLN
11   ALAGLYALASERGLYTYRVALVALLYSPRO
12   PHETHRALAALATHRLEUGLUGLULYSLEU
13   ASNLYSILEPHEGLULYSLEUGLYMET

Entity 2, beryllofluoride - Be F2 - 47.009 Da.

1   BF2

Samples:

sample_1: Chemotaxis protein Y, [U-99% 13C; U-99% 15N], 1 – 4 mM

sample_2: Chemotaxis protein Y, [U-99% 15N], 1 – 4 mM

sample_3: Chemotaxis protein Y, [U-10% 13C], 1 – 4 mM

Ex-cond_1: pH: 6.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableEx-cond_1
CBCACONHnot availablenot availableEx-cond_1
1H-15N NOESYnot availablenot availableEx-cond_1
1H-15N TOCSYnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
4D 1H-13C HMQC-NOESY-HMQCnot availablenot availableEx-cond_1
4D 1H-15N HMQC-NOESY-HSQCnot availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 2950 2951 3440 4083
PDB
DBJ BAA15698 BAB36015 BAG77641 BAI25973 BAI30936
EMBL CAD05667 CAP76371 CAQ32359 CAQ98822 CAR03242
GB AAA23570 AAA23577 AAA27037 AAC74952 AAG56872
PIR AH0745
REF NP_310619 NP_416396 NP_456482 NP_460873 NP_707777
SP P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69
AlphaFold P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks