BMRB Entry 4067

Title:
Complete 1H and Partial 13C Resonance Assignments at 37 and 22 C for Brazzein, an Intensely Sweet Protein
Deposition date:
1997-10-20
Original release date:
1998-02-25
Authors:
Caldwell, Jane; Abildgaard, Frits; Ming, Ding; Hellekant, Goran; Markley, John
Citation:

Citation: Caldwell, Jane; Abildgaard, Frits; Ming, Ding; Hellekant, Goran; Markley, John. "Complete 1H and Partial 13C Resonance Assignments at 37 and 22 C for Brazzein, an Intensely Sweet Protein"  J. Biomol. NMR 11, 231-232 (1998).

Assembly members:

Assembly members:
brazzein, polymer, 54 residues, 6474 Da.

Natural source:

Natural source:   Common Name: Joublie fruit   Taxonomy ID: 43545   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pentadiplandra brazzeana

Experimental source:

Experimental source:   Production method: purified from natural source

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts126
1H chemical shifts1019

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1brazzein1

Entities:

Entity 1, brazzein 54 residues - 6474 Da.

1   PCAASPLYSCYSLYSLYSVALTYRGLUASN
2   TYRPROVALSERLYSCYSGLNLEUALAASN
3   GLNCYSASNTYRASPCYSLYSLEUASPLYS
4   HISALAARGSERGLYGLUCYSPHETYRASP
5   GLULYSARGASNLEUGLNCYSILECYSASP
6   TYRCYSGLUTYR

Samples:

sample_1: brazzein0.8 – 2 mM; sodium azide 1 mM; DSS 0.1 mM

sample_2: brazzein1 – 4 mM; succinic-d4 acid, [U-99% 2H], 50 mM; DSS 0.1 mM

sample_3: brazzein 8 mM; succinic-d4 acid, [U-99% 2H], 50 mM; DSS 0.1 mM; sodium azide 1 mM

conditions_set_1: pH: 3.7; temperature: 310 K

conditions_set_2: pH: 5.2; temperature: 310 K

conditions_set_3: pH: 5.2; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
Gradient enhanced 1H,13C HSQCsample_2not availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AM600 600 MHz
  • Bruker DMX600 600 MHz
  • Bruker DMX750 750 MHz

Related Database Links:

BMRB 16215 16978 18710
PDB
GB ACK76425 AGM38242
SP P56552
AlphaFold P56552