BMRB Entry 34779

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34779
MolProbity Validation Chart

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Title:
apo structure of the specific silver chaperone needed for bacterial silver resistance
Deposition date:
2022-12-08
Original release date:
2023-10-20
Authors:
Monneau, Y.; Walker, O.; Hologne, M.
Citation:

Citation: Arrault, Cyrielle; Monneau, Yoan Rocky; Martin, Marie; Cantrelle, Francois-Xavier; Boll, Emmanuelle; Chirot, Fabien; Comby Zerbino, Clothilde; Walker, Olivier; Hologne, Maggy. "The battle for silver binding: How the interplay between the SilE, SilF, and SilB proteins contributes to the silver efflux pump mechanism"  J. Biol. Chem. 299, 105004-105004 (2023).
PubMed: 37394004

Assembly members:

Assembly members:
entity_1, polymer, 100 residues, 10711.252 Da.

Natural source:

Natural source:   Common Name: Salmonella enterica subsp. enterica serovar Typhimurium   Taxonomy ID: 90371   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGMLKHISHGDMNAASDA SVQQVIKGTGIVKDIDMNSK KITISHEAIPAVGWPAMTMR FTFVNADDAINALKTGNHVD FSFIQQGNISLLKSINVTQS

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts85
1H chemical shifts561

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 100 residues - 10711.252 Da.

1   GLYALAMETGLYMETLEULYSHISILESER
2   HISGLYASPMETASNALAALASERASPALA
3   SERVALGLNGLNVALILELYSGLYTHRGLY
4   ILEVALLYSASPILEASPMETASNSERLYS
5   LYSILETHRILESERHISGLUALAILEPRO
6   ALAVALGLYTRPPROALAMETTHRMETARG
7   PHETHRPHEVALASNALAASPASPALAILE
8   ASNALALEULYSTHRGLYASNHISVALASP
9   PHESERPHEILEGLNGLNGLYASNILESER
10   LEULEULYSSERILEASNVALTHRGLNSER

Samples:

sample_1: SilF 300 uM; MES 20 mM; NaCl 100 mM

sample_2: SilF, [U-100% 13C; U-100% 15N], 500 uM; MES 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 220 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1

Software:

TopSpin v4.01, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRFAM-SPARKY, NMRFAM-SPARKY - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker 600MHz 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks