BMRB Entry 19671

Name: 3rC34Y, variant of Imunoglobulin light-chain of lambda 3
Published: 2015-04-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19671

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

3rC34Y, variant of Imunoglobulin light-chain of lambda 3

Deposition date:

2013-12-10

Original release date:

2015-04-15

Authors:

Gil-Rodriguez, Paloma; Amero, Carlos

Citation:

Citation: Gil-Rodriguez, Paloma; Amero, Carlos. "(1)H, (15)N and (13)C resonance assignments for 3rC and 3rCWP: amyloidogenic variants of imunoglobulin lambda 3 light-chain" Biomol NMR Assign. 9, 139-142 (2015).
PubMed: 24816896

Assembly members:

Assembly members:
3rC34Y, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
3rC34Y: SYELTQPPSVSVSPGQTASI TCSGDKLGDKYAYWYQQKPG QSPVLVIYQDSKRPSGIPER FSGSNSGNTATLTISGTQAM DEADYYCQAWDSSTAVVFGG GTKLTVL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	273
15N chemical shifts	91
1H chemical shifts	458

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	3rC34Y	1

Entities:

Entity 1, 3rC34Y 107 residues - Formula weight is not available

1

SER

TYR

GLU

LEU

THR

GLN

PRO

SER

VAL

2

SER

VAL

SER

PRO

GLY

GLN

THR

ALA

SER

ILE

3

THR

CYS

SER

GLY

ASP

LYS

LEU

GLY

ASP

LYS

4

TYR

ALA

TYR

TRP

TYR

GLN

LYS

PRO

GLY

5

GLN

SER

PRO

VAL

LEU

VAL

ILE

TYR

GLN

ASP

6

SER

LYS

ARG

PRO

SER

GLY

ILE

PRO

GLU

ARG

7

PHE

SER

GLY

SER

ASN

SER

GLY

ASN

THR

ALA

8

THR

LEU

THR

ILE

SER

GLY

THR

GLN

ALA

MET

9

ASP

GLU

ALA

ASP

TYR

CYS

GLN

ALA

TRP

10

ASP

SER

THR

ALA

VAL

PHE

GLY

11

GLY

THR

LYS

LEU

THR

VAL

LEU

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 20 mM; 3rC34Y, [U-15N], 1 ± 0.3 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: sodium phosphate 20 mM; sodium chloride 20 mM; 3rC34Y, [U-13C; U-15N], 1 ± 0.3 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_3: TRIS 20 mM; 3rC34Y, [U-13C; U-15N], 1 ± 0.3 mM; sodium chloride 20 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.02 M; pH: 6; pressure: 1 atm; temperature: 303 K

sample_conditions_3: ionic strength: 0.02 M; pH: 8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_2
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_3
3D HNCO	sample_3	isotropic	sample_conditions_3
3D HNCA	sample_3	isotropic	sample_conditions_3
3D HNCACB	sample_3	isotropic	sample_conditions_3
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_2	isotropic	sample_conditions_2
3D C(CO)NH	sample_2	isotropic	sample_conditions_2
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe v7.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8.4, Keller and Wuthrich - data analysis

NMR spectrometers:

Varian INOVA 700 MHz

PDB	4AIX 4AIX 4AJ0
BMRB	19641
DBJ	BAA20022 BAC01665 BAC01796
EMBL	CAA12203 CAA80216 CAA85623 CAC10553 CAC10914
GB	AAA19492 AAB00975 AAC16875 AAD01813 AAD01814
PIR	S16440 S36065
PRF	2001180C