BMRB Entry 19641

Name: 3rCWP7D, variant of Imunoglobulin light-chain of lambda 3
Published: 2015-04-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19641

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

3rCWP7D, variant of Imunoglobulin light-chain of lambda 3

Deposition date:

2013-11-27

Original release date:

2015-04-15

Authors:

Gil-Rodriguez, Paloma; Amero, Carlos

Citation:

Citation: Gil-Rodriguez, Paloma; Amero, Carlos. "(1)H, (15)N and (13)C resonance assignments for 3rC and 3rCWP: amyloidogenic variants of imunoglobulin lambda 3 light-chain" Biomol NMR Assign. 9, 139-142 (2015).
PubMed: 24816896

Assembly members:

Assembly members:
3rCWP7D, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
3rCWP7D: SYELTQDPSVSVSPGQTASI TCSGDKLGDKYAYWYQQKPG QSPVLVIYQDSKRPSGIPER FSGSNSGNTATLTISGTQAM DEADYYCQAADSSGAVVFGG GTKLTVL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	322
15N chemical shifts	107
1H chemical shifts	549

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	3rCWP7D	1

Entities:

Entity 1, 3rCWP7D 107 residues - Formula weight is not available

1

SER

TYR

GLU

LEU

THR

GLN

ASP

PRO

SER

VAL

2

SER

VAL

SER

PRO

GLY

GLN

THR

ALA

SER

ILE

3

THR

CYS

SER

GLY

ASP

LYS

LEU

GLY

ASP

LYS

4

TYR

ALA

TYR

TRP

TYR

GLN

LYS

PRO

GLY

5

GLN

SER

PRO

VAL

LEU

VAL

ILE

TYR

GLN

ASP

6

SER

LYS

ARG

PRO

SER

GLY

ILE

PRO

GLU

ARG

7

PHE

SER

GLY

SER

ASN

SER

GLY

ASN

THR

ALA

8

THR

LEU

THR

ILE

SER

GLY

THR

GLN

ALA

MET

9

ASP

GLU

ALA

ASP

TYR

CYS

GLN

ALA

10

ASP

SER

GLY

ALA

VAL

PHE

GLY

11

GLY

THR

LYS

LEU

THR

VAL

LEU

Samples:

sample_1: 3rCWP7D, [U-15N], 1 ± 0.3 mM; sodium phosphate 20 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_2: 3rCWP7D, [U-13C; U-15N], 1 ± 0.3 mM; sodium phosphate 20 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe v7.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8.4, Keller and Wuthrich - data analysis

NMR spectrometers:

Varian INOVA 700 MHz

BMRB	19671
PDB	4AIX 4AJ0
GB	AAR02898