BMRB Entry 19004

Title:
1H, 13C, and 15N backbone chemical shift assignments of the low-spin CN-bound yeast cytochrome c peroxidase with the C-terminal His-tag
Deposition date:
2013-02-05
Original release date:
2013-04-02
Authors:
Volkov, Alexander; van Nuland, Nico
Citation:

Citation: Volkov, Alexander; Wohlkonig, Alexandre; Soror, Sameh; van Nuland, Nico. "Expression, Purification, Characterization, and Solution Nuclear Magnetic Resonance Study of Highly Deuterated Yeast Cytochrome c Peroxidase with Enhanced Solubility"  Biochemistry 52, 2165-2175 (2013).
PubMed: 23517193

Assembly members:

Assembly members:
cytochrome_c_peroxidase, polymer, 300 residues, Formula weight is not available
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, 616.487 Da.
CYANIDE ION, non-polymer, 26.017 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a(+)

Data sets:
Data typeCount
1H chemical shifts267
13C chemical shifts820
15N chemical shifts267

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2cofactor_HEME2
3cofactor_CYN3

Entities:

Entity 1, protein 300 residues - Formula weight is not available

C-terminal His-tag

1   METLYSTHRLEUVALHISVALALASERVAL
2   GLULYSGLYARGSERTYRGLUASPPHEGLN
3   LYSVALTYRASNALAILEALALEULYSLEU
4   ARGGLUASPASPGLUTYRASPASNTYRILE
5   GLYTYRGLYPROVALLEUVALARGLEUALA
6   TRPHISTHRSERGLYTHRTRPASPLYSHIS
7   ASPASNTHRGLYGLYSERTYRGLYGLYTHR
8   TYRARGPHELYSLYSGLUPHEASNASPPRO
9   SERASNALAGLYLEUGLNASNGLYPHELYS
10   PHELEUGLUPROILEHISLYSGLUPHEPRO
11   TRPILESERSERGLYASPLEUPHESERLEU
12   GLYGLYVALTHRALAVALGLNGLUMETGLN
13   GLYPROLYSILEPROTRPARGCYSGLYARG
14   VALASPTHRPROGLUASPTHRTHRPROASP
15   ASNGLYARGLEUPROASPALAASPLYSASP
16   ALAASPTYRVALARGTHRPHEPHEGLNARG
17   LEUASNMETASNASPARGGLUVALVALALA
18   LEUMETGLYALAHISALALEUGLYLYSTHR
19   HISLEULYSASNSERGLYTYRGLUGLYPRO
20   TRPGLYALAALAASNASNVALPHETHRASN
21   GLUPHETYRLEUASNLEULEUASNGLUASP
22   TRPLYSLEUGLULYSASNASPALAASNASN
23   GLUGLNTRPASPSERLYSSERGLYTYRMET
24   METLEUPROTHRASPTYRSERLEUILEGLN
25   ASPPROLYSTYRLEUSERILEVALLYSGLU
26   TYRALAASNASPGLNASPLYSPHEPHELYS
27   ASPPHESERLYSALAPHEGLULYSLEULEU
28   GLUASNGLYILETHRPHEPROLYSASPALA
29   PROSERPROPHEILEPHELYSTHRLEUGLU
30   GLUGLNGLYLEUHISHISHISHISHISHIS

Entity 2, cofactor_HEME - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 3, cofactor_CYN - C N - 26.017 Da.

1   CYN

Samples:

sample_1: cytochrome c peroxidase, [U-13C; U-15N; U-2H], 1.1 – 1.25 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz

Related Database Links:

BMRB 1839 19005 19075 19076 19884 25551
PDB
DBJ GAA24787
EMBL CAA44288 CAA82145 CAY81144
GB AAA88709 AAS56247 AHY76301 AJP40095 AJS30293
REF NP_012992
SP P00431
TPG DAA09217
AlphaFold P00431

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks