BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19884

Title: Yeast cytochrome c peroxidase assignment   PubMed: 24726731

Deposition date: 2014-04-01 Original release date: 2014-04-08

Authors: Schilder, Jesika; Ubbink, Marcellus; Loehr, Frank; Schwalbe, Harald

Citation: Schilder, Jesika; Lohr, Frank; Schwalbe, Harald; Ubbink, Marcellus. "The cytochrome c peroxidase and cytochrome c encounter complex: The other side of the story."  FEBS Lett. 588, 1873-1878 (2014).

Assembly members:
High_pH, polymer, 295 residues, 33643.3701 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology' 'Saccharomyces cerevisiae' 'Saccharomyces cerevisiae' . . Saccharomyces cerevisiae 'Escherichia coli

Entity Sequences (FASTA):
High_pH: MSKTLVHVASVEKGRSYEDF QKVYNAIALKLREDDEYDNY IGYGPVLVRLAWHTSGTWDK HDNTGGSYGGTYRFKKEFND PSNAGLQNGFKFLEPIHKEF PWISSGDLFSLGGVTAVQEM QGPKIPWRAGRVDTPEDTTP DNGRLPDADKDADYVRTFFQ RLNMNDREVVALMGAHALGK THLKNSGYEGPWGAANNVFT NEFYLNLLNEDWKLEKNDAN NEQWDSKSGYMMLPTDYSLI QDPKYLSIVKEYANDQDKFF KDFSKAFEKLLENGITFPKD APSPFIFKTLEEQGL

Data sets:
Data typeCount
13C chemical shifts757
15N chemical shifts240
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CcP High pH1

Entities:

Entity 1, CcP High pH 295 residues - 33643.3701 Da.

Residues 0 - 3 (MSKT) is not native; C128A mutation

1   METSERLYSTHRLEUVALHISVALALASER
2   VALGLULYSGLYARGSERTYRGLUASPPHE
3   GLNLYSVALTYRASNALAILEALALEULYS
4   LEUARGGLUASPASPGLUTYRASPASNTYR
5   ILEGLYTYRGLYPROVALLEUVALARGLEU
6   ALATRPHISTHRSERGLYTHRTRPASPLYS
7   HISASPASNTHRGLYGLYSERTYRGLYGLY
8   THRTYRARGPHELYSLYSGLUPHEASNASP
9   PROSERASNALAGLYLEUGLNASNGLYPHE
10   LYSPHELEUGLUPROILEHISLYSGLUPHE
11   PROTRPILESERSERGLYASPLEUPHESER
12   LEUGLYGLYVALTHRALAVALGLNGLUMET
13   GLNGLYPROLYSILEPROTRPARGALAGLY
14   ARGVALASPTHRPROGLUASPTHRTHRPRO
15   ASPASNGLYARGLEUPROASPALAASPLYS
16   ASPALAASPTYRVALARGTHRPHEPHEGLN
17   ARGLEUASNMETASNASPARGGLUVALVAL
18   ALALEUMETGLYALAHISALALEUGLYLYS
19   THRHISLEULYSASNSERGLYTYRGLUGLY
20   PROTRPGLYALAALAASNASNVALPHETHR
21   ASNGLUPHETYRLEUASNLEULEUASNGLU
22   ASPTRPLYSLEUGLULYSASNASPALAASN
23   ASNGLUGLNTRPASPSERLYSSERGLYTYR
24   METMETLEUPROTHRASPTYRSERLEUILE
25   GLNASPPROLYSTYRLEUSERILEVALLYS
26   GLUTYRALAASNASPGLNASPLYSPHEPHE
27   LYSASPPHESERLYSALAPHEGLULYSLEU
28   LEUGLUASNGLYILETHRPHEPROLYSASP
29   ALAPROSERPROPHEILEPHELYSTHRLEU
30   GLUGLUGLNGLYLEU

Samples:

CcP_NMR_sample: High_pH, [U-100% 13C; U-100% 15N; U-80% 2H], 0.4 mM; NaCl 100 mM; NaPi 20 mM

ccp_assignment: ionic strength: 100.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCCcP_NMR_sampleisotropicccp_assignment
3D HNCACBCcP_NMR_sampleisotropicccp_assignment
3D HNCACcP_NMR_sampleisotropicccp_assignment
BESTTR_HN(Ca)CO_950 (H[N[ca[CO]]])CcP_NMR_sampleisotropicccp_assignment
BESTTR_HNCO_950 (H[N[trho(N)][CO]])CcP_NMR_sampleisotropicccp_assignment
3D HN(CO)CACcP_NMR_sampleisotropicccp_assignment
BESTTR_HN(COCa)Cb_600 (H[N[co[{CA|ca[C]}]]])CcP_NMR_sampleisotropicccp_assignment

Software:

CcpNmr_Analysis v2.1, CCPN - assignment

Topspin v3.1, Bruker - processing

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 600 MHz

Related Database Links:

Uniprot P00431
BMRB 1839 19004 19005 19075 19076 25551
PDB
DBJ GAA24787
EMBL CAA44288 CAA82145 CAY81144
GB AAA88709 AAS56247 AHY76301 AJP40095 AJS30293
REF NP_012992
SP P00431
TPG DAA09217

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts