BMRB Entry 18821

Name: 1H, 13C and 15N assignments of Apo-acyl carrier protein of Pseudomonas aeruginosa.
Published: 2012-12-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18821

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N assignments of Apo-acyl carrier protein of Pseudomonas aeruginosa.

Deposition date:

2012-11-05

Original release date:

2012-12-04

Authors:

Parnham, Stuart; Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei

Citation:

Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1." Biomol. NMR Assignments 5, 131-133 (2011).
PubMed: 21152998

Assembly members:

Assembly members:
ApoAcpP, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ApoAcpP: GSHMSTIEERVKKIVAEQLG VKEEEVTNSASFVEDLGADS LDTVELVMALEEEFETEIPD EKAEKITTVQEAIDYIVAHQ Q

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	337
15N chemical shifts	82
1H chemical shifts	547

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ApoAcpP	1

Entities:

Entity 1, ApoAcpP 81 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

THR

ILE

GLU

ARG

2

VAL

LYS

ILE

VAL

ALA

GLU

GLN

LEU

GLY

3

VAL

LYS

GLU

VAL

THR

ASN

SER

ALA

4

SER

PHE

VAL

GLU

ASP

LEU

GLY

ALA

ASP

SER

5

LEU

ASP

THR

VAL

GLU

LEU

VAL

MET

ALA

LEU

6

GLU

PHE

GLU

THR

GLU

ILE

PRO

ASP

7

GLU

LYS

ALA

GLU

LYS

ILE

THR

VAL

GLN

8

GLU

ALA

ILE

ASP

TYR

ILE

VAL

ALA

HIS

GLN

9

GLN

Samples:

15N-ApoAcpP: ApoAcpP, [U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; D2O 10%; H2O 90%

13C15N-ApoAcpP: ApoAcpP, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 70 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N-ApoAcpP	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	13C15N-ApoAcpP	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D HNCACB	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D HNCO	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D HBHA(CO)NH	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D H(CCO)NH	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D HCCH-TOCSY	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N-ApoAcpP	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	13C15N-ApoAcpP	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	13C15N-ApoAcpP	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0.4, Bruker Biospin - collection, processing

CcpNMR v2.2.2, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 850 MHz

BMRB	18331
DBJ	BAK90915 BAP22431 BAP50057 BAQ38943 BAR67004
EMBL	CAW26824 CCQ85094 CDF85073 CDH70383 CDH76426
GB	AAB94392 AAG06354 ABJ12204 ABR81030 AEO74481
REF	NP_251656 WP_003091135 WP_007160597 WP_015477931
SP	O54439
AlphaFold	O54439