BMRB Entry 17899

Title:
1H, 13C and 15N NMR assignments of the aciniform spidroin (AcSp1) repetitive domain of Argiope trifasciata wrapping silk
Deposition date:
2011-08-29
Original release date:
2011-10-25
Authors:
Xu, Lingling; Tremblay, Marie-Laurence; Meng, Qing; Liu, Xiang-Qin; Rainey, Jan
Citation:

Citation: Xu, Lingling; Tremblay, Marie-Laurence; Meng, Qing; Liu, Xiang-Qin; Rainey, Jan. "1H, 13C and 15N NMR assignments of the aciniform spidroin (AcSp1) repetitive domain of Argiope trifasciata wrapping silk."  Biomol. NMR Assignments 6, 147-151 (2012).
PubMed: 21989955

Assembly members:

Assembly members:
W1, polymer, 199 residues, 18976.5732 Da.

Natural source:

Natural source:   Common Name: Banded garden spider   Taxonomy ID: 156845   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Argiope Trifasciata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data typeCount
13C chemical shifts689
15N chemical shifts208
1H chemical shifts1092

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1W11

Entities:

Entity 1, W1 199 residues - 18976.5732 Da.

1   ALAGLYPROGLNGLYGLYPHEGLYALATHR
2   GLYGLYALASERALAGLYLEUILESERARG
3   VALALAASNALALEUALAASNTHRSERTHR
4   LEUARGTHRVALLEUARGTHRGLYVALSER
5   GLNGLNILEALASERSERVALVALGLNARG
6   ALAALAGLNSERLEUALASERTHRLEUGLY
7   VALASPGLYASNASNLEUALAARGPHEALA
8   VALGLNALAVALSERARGLEUPROALAGLY
9   SERASPTHRSERALATYRALAGLNALAPHE
10   SERSERALALEUPHEASNALAGLYVALLEU
11   ASNALASERASNILEASPTHRLEUGLYSER
12   ARGVALLEUSERALALEULEUASNGLYVAL
13   SERSERALAALAGLNGLYLEUGLYILEASN
14   VALASPSERGLYSERVALGLNSERASPILE
15   SERSERSERSERSERPHELEUSERTHRSER
16   SERSERSERALASERTYRSERGLNALASER
17   ALASERSERTHRSERGLYALAGLYTYRTHR
18   GLYPROSERGLYPROSERTHRGLYPROSER
19   GLYTYRPROGLYPROLEUGLYGLYGLYALA
20   PROPHEGLYGLNSERGLYPHEGLYGLY

Samples:

W1_1: W1, [U-13C; U-15N], 0.76 ± 5e-05 mM; Acetate Buffer 20 mM; NaN3 1 mM; DSS 1 mM

sample1: ionic strength: 0.100 M; pH: 5.020; pressure: 1.000 atm; temperature: 303.150 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCW1_1isotropicsample1
3D HNCOW1_1isotropicsample1
3D HNCACBW1_1isotropicsample1
3D HNCAW1_1isotropicsample1
HNcaCO (H[N[ca[CO]]])W1_1isotropicsample1
HNcoCA (H[N[co[{CA|ca[C]}]]])W1_1isotropicsample1
3D HNHBW1_1isotropicsample1
HNHA (H{[N]+[HA]})W1_1isotropicsample1
ChHN (h[C]_H[N].through-space)W1_1isotropicsample1
3D HCCH-TOCSYW1_1isotropicsample1

Software:

NMRpipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr_Analysis v2.2.1, CCPN - Data Analysis

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB TmpAcc 25197
PDB
GB AAR83925

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks