BMRB Entry 25197

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25197

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Title:
wrapping silk W2
Deposition date:
2014-09-03
Original release date:
2015-09-04
Authors:
Tremblay, Marie-Laurence; Rainey, Jan; Liu, Xiang-Qin; Xu, Lingling; Meng, Qing
Citation:

Citation: Tremblay, Marie-Laurence; Xu, Lingling; Lefevre, Thierry; Sarker, Muzaddid; Orrell, Kathleen; Leclerc, Jereie; Meng, Qing; Pezolet, Michel; Auger, Michele; Liu, Xiang-Qin; Rainey, Jan. "Spider wrapping silk fibre architecture arising from its modular soluble protein precursor"  Sci. Rep. 5, 11502-11502 (2015).
PubMed: 26112753

Assembly members:

Assembly members:
W2, polymer, 399 residues, 38022.2092 Da.

Natural source:

Natural source:   Common Name: spider   Taxonomy ID: 156845   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Argiope trifasciata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET31

Entity Sequences (FASTA):

Entity Sequences (FASTA):
W2: AGPQGGFGATGGASAGLISR VANALANTSTLRTVLRTGVS QQIASSVVQRAAQSLASTLG VDGNNLARFAVQAVSRLPAG SDTSAYAQAFSSALFNAGVL NASNIDTLGSRVLSALLNGV SSAAQGLGINVDSGSVQSDI SSSSSFLSTSSSSASYSQAS ASSTSGAGYTGPSGPSTGPS GYPGPLGGGAPFGQSGFGGS AGPQGGFGATGGASAGLISR VANALANTSTLRTVLRTGVS QQIASSVVQRAAQSLASTLG VDGNNLARFAVQAVSRLPAG SDTSAYAQAFSSALFNAGVL NASNIDTLGSRVLSALLNGV SSAAQGLGINVDSGSVQSDI SSSSSFLSTSSSSASYSQAS ASSTSGAGYTGPSGPSTGPS GYPGPLGGGAPFGQSGFGG

Data sets:
Data typeCount
13C chemical shifts786
15N chemical shifts374
1H chemical shifts374

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AcSp1 W21

Entities:

Entity 1, AcSp1 W2 399 residues - 38022.2092 Da.

as is

1   ALAGLYPROGLNGLYGLYPHEGLYALATHR
2   GLYGLYALASERALAGLYLEUILESERARG
3   VALALAASNALALEUALAASNTHRSERTHR
4   LEUARGTHRVALLEUARGTHRGLYVALSER
5   GLNGLNILEALASERSERVALVALGLNARG
6   ALAALAGLNSERLEUALASERTHRLEUGLY
7   VALASPGLYASNASNLEUALAARGPHEALA
8   VALGLNALAVALSERARGLEUPROALAGLY
9   SERASPTHRSERALATYRALAGLNALAPHE
10   SERSERALALEUPHEASNALAGLYVALLEU
11   ASNALASERASNILEASPTHRLEUGLYSER
12   ARGVALLEUSERALALEULEUASNGLYVAL
13   SERSERALAALAGLNGLYLEUGLYILEASN
14   VALASPSERGLYSERVALGLNSERASPILE
15   SERSERSERSERSERPHELEUSERTHRSER
16   SERSERSERALASERTYRSERGLNALASER
17   ALASERSERTHRSERGLYALAGLYTYRTHR
18   GLYPROSERGLYPROSERTHRGLYPROSER
19   GLYTYRPROGLYPROLEUGLYGLYGLYALA
20   PROPHEGLYGLNSERGLYPHEGLYGLYSER
21   ALAGLYPROGLNGLYGLYPHEGLYALATHR
22   GLYGLYALASERALAGLYLEUILESERARG
23   VALALAASNALALEUALAASNTHRSERTHR
24   LEUARGTHRVALLEUARGTHRGLYVALSER
25   GLNGLNILEALASERSERVALVALGLNARG
26   ALAALAGLNSERLEUALASERTHRLEUGLY
27   VALASPGLYASNASNLEUALAARGPHEALA
28   VALGLNALAVALSERARGLEUPROALAGLY
29   SERASPTHRSERALATYRALAGLNALAPHE
30   SERSERALALEUPHEASNALAGLYVALLEU
31   ASNALASERASNILEASPTHRLEUGLYSER
32   ARGVALLEUSERALALEULEUASNGLYVAL
33   SERSERALAALAGLNGLYLEUGLYILEASN
34   VALASPSERGLYSERVALGLNSERASPILE
35   SERSERSERSERSERPHELEUSERTHRSER
36   SERSERSERALASERTYRSERGLNALASER
37   ALASERSERTHRSERGLYALAGLYTYRTHR
38   GLYPROSERGLYPROSERTHRGLYPROSER
39   GLYTYRPROGLYPROLEUGLYGLYGLYALA
40   PROPHEGLYGLNSERGLYPHEGLYGLY

Samples:

W2-1: W2, [U-13C; U-15N], 0.2 mM; DSS 1 mM; sodium azide 1 mM; sodium acetate, [U-2H], 20 mM

W2-2: W2, [U-13C; U-15N], 0.2 mM; DSS 1 mM; sodium azide 1 mM; sodium acetate, [U-2H], 20 mM

sample1: ionic strength: 0.100 M; pH: 5.020; pressure: 1.000 atm; temperature: 303.150 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOW2-1isotropicsample1
3D HN(CO)CAW2-1isotropicsample1
3D HNCAW2-1isotropicsample1
2D 1H-15N HSQC/HMQCW2-1isotropicsample1
3D HN(CA)COW2-1isotropicsample1
2D 1H-15N HSQC/HMQCW2-2isotropicsample1
3D HNCAW2-2isotropicsample1
3D HN(CO)CAW2-2isotropicsample1
3D HN(CA)COW2-2isotropicsample1
3D HNCOW2-2isotropicsample1

Software:

CcpNmr_Analysis v2.2, CCPN - Spectrum display, data Analysis

NMRpipe v1.0, Dr. Delaglio and Dr. Bax - Data processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks