BMRB Entry 16864

Title:
Sequence Specific 1H, 13C and 15N backbone resonance assignments of uvi31+ from Chlamydomonas reinhardtii
Deposition date:
2010-04-14
Original release date:
2010-06-15
Authors:
Rout, Ashok; Minda, Renu; Peri, Dinakar; Bhattacharya, S; Rao, Basuthkar; Chary, Kandala
Citation:

Citation: Rout, Ashok; Minda, R.; Peri, D.; Ramakrishnan, V.; Bhattacharjee, S.; Rao, B.; Chary, K.. "Sequence specific 1H, 13C and 15N backbone resonance assignments of UVI31+ from Chlamydomonas reinhardtii."  Biomol. NMR Assignments 4, 171-174 (2010).
PubMed: 20526700

Assembly members:

Assembly members:
uvi31+, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: green algae   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30-UA

Data sets:
Data typeCount
13C chemical shifts401
15N chemical shifts111
1H chemical shifts494

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1uvi31+1

Entities:

Entity 1, uvi31+ 120 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERHISVALILESERSERILEALASER
3   METALAGLUHISGLNLEUGLYPROILEALA
4   GLYALAILELYSSERLYSVALGLUALAALA
5   LEUSERPROTHRHISPHELYSLEUILEASN
6   ASPSERHISLYSHISALAGLYHISTYRALA
7   ARGASPGLYSERTHRALASERASPALAGLY
8   GLUTHRHISPHEARGLEUGLUVALTHRSER
9   ASPALAPHELYSGLYLEUTHRLEUVALLYS
10   ARGHISGLNLEUILETYRGLYLEULEUSER
11   ASPGLUPHELYSALAGLYLEUHISALALEU
12   SERMETTHRTHRLYSTHRPROALAGLUGLN

Samples:

sample_1: uvi31+, [U-100% 15N], 0.8 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; NaCl 50 mM

sample_2: uvi31+, [U-100% 13C; U-100% 15N], 0.7 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - processing

FELIX, Accelrys Software Inc. - processing

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19307 19308
PDB
GB EDO96758
REF XP_001702905

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks