BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16613

Title: SARS Coronavirus-unique domain (SUD): Three-domain molecular architecture in solution and RNA binding   PubMed: 20493876

Deposition date: 2009-11-19 Original release date: 2010-06-15

Authors: Johnson, Margaret; Chatterjee, Amarnath; Wuthrich, Kurt

Citation: Johnson, Margaret; Chatterjee, Amarnath; Neuman, Benjamin; Wuthrich, Kurt. "SARS coronavirus unique domain: three-domain molecular architecture in solution and RNA binding."  J. Mol. Biol. 400, 724-742 (2010).

Assembly members:
SUD-MC, polymer, 198 residues, Formula weight is not available

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: Viruses   Kingdom: not available   Genus/species: Coronavirus SARS coronavirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Entity Sequences (FASTA):
SUD-MC: GSHMGTVSWNLREMLAHAEE TRKLMPICMDVRAIMATIQR KYKGIKIQEGIVDYGVRFFF YTSKEPVASIITKLNSLNEP LVTMPIGYVTHGFNLEEAAR CMRSLKAPAVVSVSSPDAVT TYNGYLTSSSKTSEEHFVET VSLAGSYRDWSYSGQRTELG VEFLKRGDKIVYHTLESPVE FHLDGEVLSLDKLKSLLS

Data sets:
Data typeCount
13C chemical shifts675
15N chemical shifts201
1H chemical shifts1407

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SUD-MC1

Entities:

Entity 1, SUD-MC 198 residues - Formula weight is not available

Residues 1-4 represent a vector-derived tag. Residues 5-198 represent two domains of a multidomain protein. Residue 178 (PRO 700) is in cis conformation.

1   GLYSERHISMETGLYTHRVALSERTRPASN
2   LEUARGGLUMETLEUALAHISALAGLUGLU
3   THRARGLYSLEUMETPROILECYSMETASP
4   VALARGALAILEMETALATHRILEGLNARG
5   LYSTYRLYSGLYILELYSILEGLNGLUGLY
6   ILEVALASPTYRGLYVALARGPHEPHEPHE
7   TYRTHRSERLYSGLUPROVALALASERILE
8   ILETHRLYSLEUASNSERLEUASNGLUPRO
9   LEUVALTHRMETPROILEGLYTYRVALTHR
10   HISGLYPHEASNLEUGLUGLUALAALAARG
11   CYSMETARGSERLEULYSALAPROALAVAL
12   VALSERVALSERSERPROASPALAVALTHR
13   THRTYRASNGLYTYRLEUTHRSERSERSER
14   LYSTHRSERGLUGLUHISPHEVALGLUTHR
15   VALSERLEUALAGLYSERTYRARGASPTRP
16   SERTYRSERGLYGLNARGTHRGLULEUGLY
17   VALGLUPHELEULYSARGGLYASPLYSILE
18   VALTYRHISTHRLEUGLUSERPROVALGLU
19   PHEHISLEUASPGLYGLUVALLEUSERLEU
20   ASPLYSLEULYSSERLEULEUSER

Samples:

sample_1: SUD-MC, [U-98% 13C; U-98% 15N], 1.0 mM; sodium phosphate 25 mM; sodium chloride 150 mM; sodium azide 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.227 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D [15N,1H]-HSQCsample_1isotropicsample_conditions_1
2D [13C,1H]-HSQCsample_1isotropicsample_conditions_1
3D 15N-resolved [1H,1H-NOESYsample_1isotropicsample_conditions_1
3D aliphatic 13C-resolved [1H,1H]-NOESYsample_1isotropicsample_conditions_1
4D APSY-HACANHsample_1isotropicsample_conditions_1
5D APSY-CBCACONHsample_1isotropicsample_conditions_1
5D APSY-HACACONHsample_1isotropicsample_conditions_1
4D APSY-HNCOCAsample_1isotropicsample_conditions_1
3D aromatic 13C-resolved [1H,1H]-NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CARA, Keller and Wuthrich - chemical shift assignment

ASCAN, Fiorito F, Herrmann T, Damberger FF, Wuthrich K. - chemical shift assignment

MATCH, Volk, J., Herrmann, T., Wuthrich, K - chemical shift assignment

ATNOS, Herrmann T, Guntert P, Wuthrich K - peak picking

CANDID, Herrmann T, Guntert P, Wuthrich K - structure solution

OpalP, Luginbuhl P, Guntert P, Billeter M, Wuthrich K. - refinement

UNIO, Herrmann T, Wuthrich K - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15469 15618
PDB
DBJ BAC81346 BAC81347 BAC81360 BAC81361 BAC81374
GB AAP13439 AAP13442 AAP13566 AAP13575 AAP30028
REF NP_828849 NP_828850 NP_828862
SP P0C6U8 P0C6X7

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts