BMRB Entry 16395

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16395

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Assignments of M.HhaI bound with hemimethylated DNA
Deposition date:
2009-07-07
Original release date:
2009-09-04
Authors:
Zhou, Hongjun; Murdy, Matthew; Dahlquist, Frederick; Reich, Norbert
Citation:

Citation: Zhou, Hongjun; Murdy, Matthew; Dahlquist, Frederick; Reich, Norbert. "The recognition Pathway for the DNA Cytosine Methyltransferase M.HhaI"  Biochemistry 48, 7807-7816 (2009).
PubMed: 19580326

Assembly members:

Assembly members:
M.HhaI, polymer, 331 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M.HhaI: MIEIKDKQLTGLRFIDLFAG LGGFRLALESCGAECVYSNE WDKYAQEVYEMNFGEKPEGD ITQVNEKTIPDHDILCAGFP CQAFSISGKQKGFEDSRGTL FFDIARIVREKKPKVVFMEN VKNFASHDNGNTLEVVKNTM NELDYSFHAKVLNALDYGIP QKRERIYMICFRNDLNIQNF QFPKPFELNTFVKDLLLPDS EVEHLVIDRKDLVMTNQEIE QTTPKTVRLGIVGKGGQGER IYSTRGIAITLSAYGGGIFA KTGGYLVNGKTRKLHPRECA RVMGYPDSYKVHPSTSQAYK QFGNSVVINVLQYIAYNIGS SLNLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts557
15N chemical shifts276
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 331 residues - Formula weight is not available

1   METILEGLUILELYSASPLYSGLNLEUTHR
2   GLYLEUARGPHEILEASPLEUPHEALAGLY
3   LEUGLYGLYPHEARGLEUALALEUGLUSER
4   CYSGLYALAGLUCYSVALTYRSERASNGLU
5   TRPASPLYSTYRALAGLNGLUVALTYRGLU
6   METASNPHEGLYGLULYSPROGLUGLYASP
7   ILETHRGLNVALASNGLULYSTHRILEPRO
8   ASPHISASPILELEUCYSALAGLYPHEPRO
9   CYSGLNALAPHESERILESERGLYLYSGLN
10   LYSGLYPHEGLUASPSERARGGLYTHRLEU
11   PHEPHEASPILEALAARGILEVALARGGLU
12   LYSLYSPROLYSVALVALPHEMETGLUASN
13   VALLYSASNPHEALASERHISASPASNGLY
14   ASNTHRLEUGLUVALVALLYSASNTHRMET
15   ASNGLULEUASPTYRSERPHEHISALALYS
16   VALLEUASNALALEUASPTYRGLYILEPRO
17   GLNLYSARGGLUARGILETYRMETILECYS
18   PHEARGASNASPLEUASNILEGLNASNPHE
19   GLNPHEPROLYSPROPHEGLULEUASNTHR
20   PHEVALLYSASPLEULEULEUPROASPSER
21   GLUVALGLUHISLEUVALILEASPARGLYS
22   ASPLEUVALMETTHRASNGLNGLUILEGLU
23   GLNTHRTHRPROLYSTHRVALARGLEUGLY
24   ILEVALGLYLYSGLYGLYGLNGLYGLUARG
25   ILETYRSERTHRARGGLYILEALAILETHR
26   LEUSERALATYRGLYGLYGLYILEPHEALA
27   LYSTHRGLYGLYTYRLEUVALASNGLYLYS
28   THRARGLYSLEUHISPROARGGLUCYSALA
29   ARGVALMETGLYTYRPROASPSERTYRLYS
30   VALHISPROSERTHRSERGLNALATYRLYS
31   GLNPHEGLYASNSERVALVALILEASNVAL
32   LEUGLNTYRILEALATYRASNILEGLYSER
33   SERLEUASNLEUGLUHISHISHISHISHIS
34   HIS

Samples:

sample_1: M.HhaI, [U-13C; U-15N; U-2H], 0.5 mM; H2O 90%; D2O 10%; NaCl 100 mM; NaPi 50 mM; glycerol 0.75%; arg 50 mM; Glu 50 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15170
PDB
GB AAA24989 EIJ69210
REF WP_005706946
SP P05102
AlphaFold P05102

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks