BMRB Entry 15170

Name: Backbone assignments of DNA methyltransferase M.HhaI
Published: 2007-06-06

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15170

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of DNA methyltransferase M.HhaI

Deposition date:

2007-03-09

Original release date:

2007-06-06

Authors:

Zhou, Hongjun; Shatz, Whitney; Purdy, Matthew; Fera, Nick; Dahlquist, Frederick; Reich, Norbert

Citation:

Citation: Zhou, Hongjun; Shatz, Whitney; Purdy, Matthew; Fera, Nick; Dahlquist, Frederick; Reich, Norbert. "Long-range structural and dynamical changes induced by cofactor binding in DNA methyltransferase M.HhaI." Biochemistry 46, 7261-7268 (2007).
PubMed: 17523600

Assembly members:

Assembly members:
M.HhaI, polymer, 331 residues, Formula weight is not available
SAH, non-polymer, 384.411 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M.HhaI: MIEIKDKQLTGLRFIDLFAG LGGFRLALESCGAECVYSNE WDKYAQEVYEMNFGEKPEGD ITQVNEKTIPDHDILCAGFP CQAFSISGKQKGFEDSRGTL FFDIARIVREKKPKVVFMEN VKNFASHDNGNTLEVVKNTM NELDYSFHAKVLNALDYGIP QKRERIYMICFRNDLNIQNF QFPKPFELNTFVKDLLLPDS EVEHLVIDRKDLVMTNQEIE QTTPKTVRLGIVGKGGQGER IYSTRGIAITLSAYGGGIFA KTGGYLVNGKTRKLHPRECA RVMGYPDSYKVHPSTSQAYK QFGNSVVINVLQYIAYNIGS SLNLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	483
15N chemical shifts	242
1H chemical shifts	242

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer	1
2	SAH	2

Entities:

Entity 1, monomer 331 residues - Formula weight is not available

1

MET

ILE

GLU

ILE

LYS

ASP

LYS

GLN

LEU

THR

2

GLY

LEU

ARG

PHE

ILE

ASP

LEU

PHE

ALA

GLY

3

LEU

GLY

PHE

ARG

LEU

ALA

LEU

GLU

SER

4

CYS

GLY

ALA

GLU

CYS

VAL

TYR

SER

ASN

GLU

5

TRP

ASP

LYS

TYR

ALA

GLN

GLU

VAL

TYR

GLU

6

MET

ASN

PHE

GLY

GLU

LYS

PRO

GLU

GLY

ASP

7

ILE

THR

GLN

VAL

ASN

GLU

LYS

THR

ILE

PRO

8

ASP

HIS

ASP

ILE

LEU

CYS

ALA

GLY

PHE

PRO

9

CYS

GLN

ALA

PHE

SER

ILE

SER

GLY

LYS

GLN

10

LYS

GLY

PHE

GLU

ASP

SER

ARG

GLY

THR

LEU

11

PHE

ASP

ILE

ALA

ARG

ILE

VAL

ARG

GLU

12

LYS

PRO

LYS

VAL

PHE

MET

GLU

ASN

13

VAL

LYS

ASN

PHE

ALA

SER

HIS

ASP

ASN

GLY

14

ASN

THR

LEU

GLU

VAL

LYS

ASN

THR

MET

15

ASN

GLU

LEU

ASP

TYR

SER

PHE

HIS

ALA

LYS

16

VAL

LEU

ASN

ALA

LEU

ASP

TYR

GLY

ILE

PRO

17

GLN

LYS

ARG

GLU

ARG

ILE

TYR

MET

ILE

CYS

18

PHE

ARG

ASN

ASP

LEU

ASN

ILE

GLN

ASN

PHE

19

GLN

PHE

PRO

LYS

PRO

PHE

GLU

LEU

ASN

THR

20

PHE

VAL

LYS

ASP

LEU

PRO

ASP

SER

21

GLU

VAL

GLU

HIS

LEU

VAL

ILE

ASP

ARG

LYS

22

ASP

LEU

VAL

MET

THR

ASN

GLN

GLU

ILE

GLU

23

GLN

THR

PRO

LYS

THR

VAL

ARG

LEU

GLY

24

ILE

VAL

GLY

LYS

GLY

GLN

GLY

GLU

ARG

25

ILE

TYR

SER

THR

ARG

GLY

ILE

ALA

ILE

THR

26

LEU

SER

ALA

TYR

GLY

ILE

PHE

ALA

27

LYS

THR

GLY

TYR

LEU

VAL

ASN

GLY

LYS

28

THR

ARG

LYS

LEU

HIS

PRO

ARG

GLU

CYS

ALA

29

ARG

VAL

MET

GLY

TYR

PRO

ASP

SER

TYR

LYS

30

VAL

HIS

PRO

SER

THR

SER

GLN

ALA

TYR

LYS

31

GLN

PHE

GLY

ASN

SER

VAL

ILE

ASN

VAL

32

LEU

GLN

TYR

ILE

ALA

TYR

ASN

ILE

GLY

SER

33

SER

LEU

ASN

LEU

GLU

HIS

34

HIS

Entity 2, SAH - C14 H20 N6 O5 S - 384.411 Da.

1

SAH

Samples:

sample_1: M.HhaI, [U-13C; U-15N; U-2H], 0.3 – 0.6 mM; sodium phosphate 50 mM; DTT 5 mM; sodium chloride 100 mM; Arg 50 mM; Glu 50 mM; sodium azide 0.02%; glycerol 0.75%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	16395
PDB	10MH 1FJX 1HMY 1M0E 1MHT 1SKM 1SVU 2C7O 2C7P 2C7Q 2C7R 2HMY 2HR1 2I9K 2UYC 2UYH 2UZ4 2Z6A 2Z6Q 2Z6U 2ZCJ 3EEO 3MHT 4MHT 5MHT 6MHT 7MHT 8MHT 9MHT
GB	AAA24989 EIJ69210
REF	WP_005706946
SP	P05102
AlphaFold	P05102