BMRB Entry 16334

Title:
Sequence specific backbone resonance assignment of Neuronal Calcium Sensor 1(NCS1) in non-myristoylated form.
Deposition date:
2009-06-05
Original release date:
2010-07-08
Authors:
Chary, Kandala; Chandra, Kousik; Sharma, Yogendra
Citation:

Citation: Chandra, Kousik; Ramakrishnan, Venkatesh; Sharma, Yogendra; Chary, Kandala. "Resonance assignments of myristoylated and non-myristoylated neuronal calcium sensor-1(NCS-1) embedded in a membrane"  Biomol. NMR Assignments 4, 155-158 (2010).
PubMed: 20490953

Assembly members:

Assembly members:
Nonmyristoylated_Neuronal_Calcium_Sensor1, polymer, 190 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway Rat   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Data sets:
Data typeCount
13C chemical shifts502
15N chemical shifts175
1H chemical shifts853

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EF- hand protein1

Entities:

Entity 1, EF- hand protein 190 residues - Formula weight is not available

1   METGLYLYSSERASNSERLYSLEULYSPRO
2   GLUVALVALGLUGLULEUTHRARGLYSTHR
3   TYRPHETHRGLULYSGLUVALGLNGLNTRP
4   TYRLYSGLYPHEILELYSASPCYSPROSER
5   GLYGLNLEUASPALAALAGLYPHEGLNLYS
6   ILETYRLYSGLNPHEPHEPROPHEGLYASP
7   PROTHRLYSPHEALATHRPHEVALPHEASN
8   VALPHEASPGLUASNLYSASPGLYARGILE
9   GLUPHESERGLUPHEILEGLNALALEUSER
10   VALTHRSERARGGLYTHRLEUASPGLULYS
11   LEUARGTRPALAPHELYSLEUTYRASPLEU
12   ASPASNASPGLYTYRILETHRARGASNGLU
13   METLEUASPILEVALASPALAILETYRGLN
14   METVALGLYASNTHRVALGLULEUPROGLU
15   GLUGLUASNTHRPROGLULYSARGVALASP
16   ARGILEPHEALAMETMETASPLYSASNALA
17   ASPGLYLYSLEUTHRLEUGLNGLUPHEGLN
18   GLUGLYSERLYSALAASPPROSERILEVAL
19   GLNALALEUSERLEUTYRASPGLYLEUVAL

Samples:

sample_1: Nonmyristoylated Neuronal Calcium Sensor1, [U-100% 13C; U-100% 15N], 0.8 – 1 mM; H2O 90%; D2O 10%; TRIS 50 mM; potassium chloride 100 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

CARA v1.8.4.2, Rochus Keller - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16726 4378
PDB
DBJ BAC37557 BAG73169
EMBL CAA57678 CAA58867 CAG05167 CAH90617 CAJ81731
GB AAA85633 AAA88510 AAC59690 AAD01642 AAF01804
REF NP_001018350 NP_001035637 NP_001084088 NP_001085136 NP_001122298
SP P62166 P62167 P62168 Q2V8Y7 Q5RC90
TPG DAA24173
AlphaFold P62166 P62167 P62168 Q2V8Y7 Q5RC90

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks