BMRB Entry 15626

Title:
backbone assignment of yeast (Saccharomyces cerevisiae) ARF1 ,non-myristoylated, GDP-bound
Deposition date:
2008-01-15
Original release date:
2008-06-30
Authors:
Liu, Yizhou
Citation:

Citation: Liu, Yizhou; Prestegard, James. "Direct measurement of dipole-dipole/CSA cross-correlated relaxation by a constant-time experiment"  J. Magn. Reson. 193, 23-31 (2008).
PubMed: 18406649

Assembly members:

Assembly members:
yeast_ARF1, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3C

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts155
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yeast ARF11

Entities:

Entity 1, yeast ARF1 188 residues - Formula weight is not available

1   GLYLEUPHEALASERLYSLEUPHESERASN
2   LEUPHEGLYASNLYSGLUMETARGILELEU
3   METVALGLYLEUASPGLYALAGLYLYSTHR
4   THRVALLEUTYRLYSLEULYSLEUGLYGLU
5   VALILETHRTHRILEPROTHRILEGLYPHE
6   ASNVALGLUTHRVALGLNTYRLYSASNILE
7   SERPHETHRVALTRPASPVALGLYGLYGLN
8   ASPARGILEARGSERLEUTRPARGHISTYR
9   TYRARGASNTHRGLUGLYVALILEPHEVAL
10   VALASPSERASNASPARGSERARGILEGLY
11   GLUALAARGGLUVALMETGLNARGMETLEU
12   ASNGLUASPGLULEUARGASNALAALATRP
13   LEUVALPHEALAASNLYSGLNASPLEUPRO
14   GLUALAMETSERALAALAGLUILETHRGLU
15   LYSLEUGLYLEUHISSERILEARGASNARG
16   PROTRPPHEILEGLNALATHRCYSALATHR
17   SERGLYGLUGLYLEUTYRGLUGLYLEUGLU
18   TRPLEUSERASNSERLEULYSASNSERTHR
19   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: yeast ARF1, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15809
PDB
DBJ GAA22060
EMBL CAA58255 CAA98769 CAG60356 CAY78316 CCC67945
GB AAA34431 AHY74824 AJP37565 AJU57677 AJU58380
REF NP_010089 XP_001646910 XP_003667691 XP_003674325 XP_003676799
SP P11076
TPG DAA11671
AlphaFold P11076

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks