BMRB Entry 15581

Title:
N-terminal domain of Mason-Pfizer monkey virus capsid protein, CA 1-140
Deposition date:
2007-12-05
Original release date:
2008-03-31
Authors:
Macek, Pavel; Zidek, Lukas; Rumlova, Michaela; Sklenar, Vladimir; Pichova, Iva
Citation:

Citation: Macek, Pavel; Zidek, Lukas; Rumlova, Michaela; Pichova, Iva; Sklenar, Vladimir. "1H, 13C, and 15N resonance assignment of the N-terminal domain of Mason-Pfizer monkey virus capsid protein, CA 1-140"  Biomol. NMR Assignments 2, 43-45 (2008).
PubMed: 19636921

Assembly members:

Assembly members:
MPMV_NTD_CA, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mason-Pfizer monkey virus   Taxonomy ID: 11855   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betaretrovirus Mason-Pfizer monkey virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: CANTDpET22b

Data sets:
Data typeCount
13C chemical shifts564
15N chemical shifts151
1H chemical shifts879

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MPMV NTD CA1

Entities:

Entity 1, MPMV NTD CA 140 residues - Formula weight is not available

1   PROVALTHRGLUTHRVALASPGLYGLNGLY
2   GLNALATRPARGHISHISASNGLYPHEASP
3   PHEALAVALILELYSGLULEULYSTHRALA
4   ALASERGLNTYRGLYALATHRALAPROTYR
5   THRLEUALAILEVALGLUSERVALALAASP
6   ASNTRPLEUTHRPROTHRASPTRPASNTHR
7   LEUVALARGALAVALLEUSERGLYGLYASP
8   HISLEULEUTRPLYSSERGLUPHEPHEGLU
9   ASNCYSARGASPTHRALALYSARGASNGLN
10   GLNALAGLYASNGLYTRPASPPHEASPMET
11   LEUTHRGLYSERGLYASNTYRSERSERTHR
12   ASPALAGLNMETGLNTYRASPPROGLYLEU
13   PHEALAGLNILEGLNALAALAALATHRLYS
14   ALATRPARGLYSLEUPROVALLYSGLYASP

Samples:

sample_1: MPMV NTD CA, [U-100% 15N], 0.7-1.0 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 8.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16234
PDB
DBJ BAD89356 BAQ21760 BAQ21764
GB AAA47710 AAA47730 AAC82573 AAC82574 AAC82576
PIR FOLJSA
REF NP_056891 NP_056892 NP_056893 NP_954560 NP_954565
SP P04022 P07567
AlphaFold P04022 P07567

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks