BMRB Entry 16234

Title:
Relaxation data of the N-terminal domain of capsid protein from the Mason-Pfizer monkey virus
Deposition date:
2009-03-31
Original release date:
2009-06-17
Authors:
Macek, Pavel; Kaderavek, Pavel; Zidek, Lukas; Sklenar, Vladimir; Macek, Pavel
Citation:

Citation: Macek, Pavel; Chmelik, Josef; Krizova, Ivana; Kaderavek, Pavel; Padrta, Petr; Zidek, Lukas; Wildova, Marcela; Hadravova, Romana; Chaloupkova, Radka; Pichova, Iva; Ruml, Tomas; Rumlova, Michaela; Sklenar, Vladimir. "NMR structure of the N-terminal domain of capsid protein from the Mason-Pfizer monkey virus"  J. Mol. Biol. 392, 100-114 (2009).
PubMed: 19527730

Assembly members:

Assembly members:
MPMV_NTD_CA, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mason-Pfizer Monkey Virus   Taxonomy ID: 11855   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betaretrovirus Mason-Pfizer monkey virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Data sets:
Data typeCount
heteronuclear NOE values111
T1 relaxation values111
T2 relaxation values111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MPMV NTD CA1

Entities:

Entity 1, MPMV NTD CA 140 residues - Formula weight is not available

1   PROVALTHRGLUTHRVALASPGLYGLNGLY
2   GLNALATRPARGHISHISASNGLYPHEASP
3   PHEALAVALILELYSGLULEULYSTHRALA
4   ALASERGLNTYRGLYALATHRALAPROTYR
5   THRLEUALAILEVALGLUSERVALALAASP
6   ASNTRPLEUTHRPROTHRASPTRPASNTHR
7   LEUVALARGALAVALLEUSERGLYGLYASP
8   HISLEULEUTRPLYSSERGLUPHEPHEGLU
9   ASNCYSARGASPTHRALALYSARGASNGLN
10   GLNALAGLYASNGLYTRPASPPHEASPMET
11   LEUTHRGLYSERGLYASNTYRSERSERTHR
12   ASPALAGLNMETGLNTYRASPPROGLYLEU
13   PHEALAGLNILEGLNALAALAALATHRLYS
14   ALATRPARGLYSLEUPROVALLYSGLYASP

Samples:

sample_1: MPMV NTD CA, [U-100% 15N], 0.7 – 1.0 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

Relax, d'Auvergne, Gooley - relaxation analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15581
PDB
DBJ BAD89356 BAQ21760 BAQ21764
GB AAA47710 AAA47730 AAC82573 AAC82574 AAC82576
PIR FOLJSA
REF NP_056891 NP_056892 NP_056893 NP_954560 NP_954565
SP P04022 P07567
AlphaFold P04022 P07567