BMRB Entry 15174

Title:
1H, alpha-13C Chemical Shift Assignments for [L-Phe44]iota-RXIA
Deposition date:
2007-03-15
Original release date:
2007-10-24
Authors:
Buczek, Olga; Wei, Daxiu; Babon, Jeffrey; Yang, Xiaodong; Fiedler, Brain; Yoshikami, Doju; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond
Citation:

Citation: Buczek, Olga; Wei, Daxiu; Babon, Jeffrey; Yang, Xiaodong; Fiedler, Brain; Chen, P.; Yoshikami, Doju; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond. "Structure and sodium channel activity of an excitatory I1-superfamily conotoxin."  Biochemistry 46, 9929-9940 (2007).
PubMed: 17696362

Assembly members:

Assembly members:
[L-Phe44]iota-RXIA_(r11a), polymer, 46 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
[L-Phe44]iota-RXIA_(r11a): GXSFCKADEKXCEYHADCCN CCLSGICAXSTNWILPGCST SSFFKI

Data sets:
Data typeCount
13C chemical shifts44
1H chemical shifts274
coupling constants33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1iota-RXIA1

Entities:

Entity 1, iota-RXIA 46 residues - Formula weight is not available

1   GLYHYPSERPHECYSLYSALAASPGLULYS
2   HYPCYSGLUTYRHISALAASPCYSCYSASN
3   CYSCYSLEUSERGLYILECYSALAHYPSER
4   THRASNTRPILELEUPROGLYCYSSERTHR
5   SERSERPHEPHELYSILE

Samples:

(L-Phe44)iota-RXIA_(r11a)sample: [L-Phe44]iota-RXIA (r11a) 1.1 mM; H2O 95%; D2O 5%

(L-Phe44)iota-RXIA: pH: 5.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESY(L-Phe44)iota-RXIA_(r11a)sampleisotropic(L-Phe44)iota-RXIA
2D 1H-1H TOCSY(L-Phe44)iota-RXIA_(r11a)sampleisotropic(L-Phe44)iota-RXIA
2D DQF-COSY(L-Phe44)iota-RXIA_(r11a)sampleisotropic(L-Phe44)iota-RXIA
2D 1H-13C HSQC(L-Phe44)iota-RXIA_(r11a)sampleisotropic(L-Phe44)iota-RXIA

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15175
PDB