BMRB Entry 15175

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15175

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
proton and alpha-carbon chemical shift of [D-Phe44]iota-RXIA
Deposition date:
2007-03-15
Original release date:
2007-10-24
Authors:
Buczek, Olga; Wei, Daxiu; Babon, Jeffrey; Yang, Xiaodong; Fiedler, Brain; Yoshikami, Doju; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond
Citation:

Citation: Buczek, Olga; Wei, Daxiu; Babon, Jeffrey; Yang, Xiaodong; Fiedler, Brain; Chen, P.; Yoshikami, Doju; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond. "Structure and sodium channel activity of an excitatory I1-superfamily conotoxin."  Biochemistry 46, 9929-9940 (2007).
PubMed: 17696362

Assembly members:

Assembly members:
[D-Phe44]iota-RXIA, polymer, 46 residues, Formula weight is not available
HYP, non-polymer, 131.130 Da.

Natural source:

Natural source:   Common Name: Conus radiatus   Taxonomy ID: 61198   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus radiatus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: Conus radiatus

Entity Sequences (FASTA):

Entity Sequences (FASTA):
[D-Phe44]iota-RXIA: GXSFCKADEKXCEYHADCCN CCLSGICAXSTNWILPGCST SSFFKI

Data sets:
Data typeCount
15N chemical shifts39
1H chemical shifts265
coupling constants33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1iota-RXIA1

Entities:

Entity 1, iota-RXIA 46 residues - Formula weight is not available

1   GLYHYPSERPHECYSLYSALAASPGLULYS
2   HYPCYSGLUTYRHISALAASPCYSCYSASN
3   CYSCYSLEUSERGLYILECYSALAHYPSER
4   THRASNTRPILELEUPROGLYCYSSERTHR
5   SERSERPHEPHELYSILE

Samples:

sample_1: [D-Phe44]iota-RXIA 0.8 mM

sample_conditions_1: pH: 5.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15174
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks