BMRB Entry 11177

Title:
Backbone chemical shift assignments for Thermus thermophilus RecO
Deposition date:
2010-07-08
Original release date:
2011-06-03
Authors:
Inoue, Jin; Nagae, Takayuki; Mishima, Masaki; Ito, Yutaka; Shibata, Takehiko; Mikawa, Tsutomu
Citation:

Citation: Inoue, Jin; Nagae, Takayuki; Mishima, Masaki; Ito, Yutaka; Shibata, Takehiko; Mikawa, Tsutomu. "A mechanism for single-stranded DNA-binding protein (SSB) displacement from single-stranded DNA upon SSB-RecO interaction."  J. Biol. Chem. 286, 6720-6732 (2011).
PubMed: 21169364

Assembly members:

Assembly members:
RecO, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermus thermophilus HB8   Taxonomy ID: 300852   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
15N chemical shifts189
1H chemical shifts189

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RecO1

Entities:

Entity 1, RecO 229 residues - Formula weight is not available

1   METALAPROALATYRTHRGLYLYSVALGLU
2   ARGTYRARGLEUGLUGLUGLYILEVALVAL
3   GLYARGLYSPROLEUPROGLNGLYASPLEU
4   LEULEUARGLEUVALTHRPROARGGLYSER
5   LEUGLUALAVALVALARGLYSGLYGLNARG
6   PROTHRGLYARGTHRGLYARGLEUSERLEU
7   PHEHISHISVALARGPHEGLNLEUTYRALA
8   LYSGLYGLUGLYLEUPROTHRLEUTHRGLN
9   ALAGLULEULEUGLYARGLEUHISGLYLEU
10   GLUALAPROARGARGPHELEULEUALAALA
11   PHELEUALAGLULEUALATYRARGLEUALA
12   SERPROGLUALAALAPROARGILETYRPRO
13   LEULEUVALSERGLYLEUARGGLYILEALA
14   LYSHISGLUASPPROLEULEUPROLEUVAL
15   TRPALAGLYTRPARGVALALALYSALAGLY
16   GLYILEGLYPROASNLEUGLUGLYGLUGLY
17   LEUARGLEULYSARGGLYARGLEUGLYGLU
18   GLUGLYVALTYRLEUGLYARGGLUGLYVAL
19   GLUALALEULYSALATHRLEUARGLEUPRO
20   GLYALAGLNALALEUPROHISLEUGLUGLY
21   ALAPROLEUASNARGLEUPHELEUALALEU
22   LYSALAHISALAGLUGLUALALEUGLYPRO
23   LEUARGSERALAGLUALAILEGLYVAL

Samples:

sample_1: RecO, [U-100% 13C; U-100% 15N], 0.4 mM; TRIS 20 mM; DTT 2 mM; EDTA 2 mM; Magnesium chloride 10 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 7.5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11047
DBJ BAD70446
GB AAS80606 AEG33049 AFH39328 EIA39541
REF WP_008632002 WP_011228077 WP_014510042 WP_014629934 WP_024119227

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks