BMRB Entry 7241

Title:
Chromo 2 domain of cpSRP43 complexed with cpSRP54 peptide
Deposition date:
2006-07-24
Original release date:
2007-07-24
Authors:
Kathir, Karuppanan; Vaithiyalingam, Sivaraja; Dakshinamurthy, Rajalingam; Henry, Ralph; Thallapuranam, Suresh Kumar
Citation:

Citation: Kathir, Karuppanan; Vaithiyalingam, Sivaraja; Dakshinamurthy, Rajalingam; Henry, Ralph; Thallapuranam, Suresh Kumar. "Structural Characterization of the Interface of the Subunits Chloroplast Signal Recognition Particle (cpSRP) ? Determination of the Three-Dimensional Solution Structure of the Chromo Domain 2 ? cpSRP54 Peptide Complex"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
CD2 of cpSRP43, polymer, 57 residues, 6539.1 Da.
cpSRP54 peptide, polymer, 14 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Arabdopsis   Taxonomy ID: 3702   Superkingdom: Eubacteria   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD2 of cpSRP43: GSQVFEYAEVDEIVEKRGKG KDVEYLVRWKDGGDCEWVKG VHVAEDVAKDYEDGLEY
cpSRP54 peptide: APPGTARRKRKADS

Data sets:
Data typeCount
13C chemical shifts151
15N chemical shifts57
1H chemical shifts306

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD2 domain of cpSRP431
2cpSRP54 peptide2

Entities:

Entity 1, CD2 domain of cpSRP43 57 residues - 6539.1 Da.

1   GLYSERGLNVALPHEGLUTYRALAGLUVAL
2   ASPGLUILEVALGLULYSARGGLYLYSGLY
3   LYSASPVALGLUTYRLEUVALARGTRPLYS
4   ASPGLYGLYASPCYSGLUTRPVALLYSGLY
5   VALHISVALALAGLUASPVALALALYSASP
6   TYRGLUASPGLYLEUGLUTYR

Entity 2, cpSRP54 peptide 14 residues - Formula weight is not available

1   ALAPROPROGLYTHRALAARGARGLYSARG
2   LYSALAASPSER

Samples:

sample_1: CD2 domain of cpSRP43, [U-13C; U-15N], 1.0 ± 0.05 mM; cpSRP54 peptide 1.0 ± 0.05 mM; Phosphate 20 mM; NaCl 50 mM; sodium azide 1 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_1

Software:

SPARKY -

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 15540
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks