BMRB Entry 15540

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15540

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Title:
1H, 15N, 13C Assignments of the C-terminal chromatin binding domain of the 43kDa subunit of chloroplast signal recognition particle
Deposition date:
2007-10-30
Original release date:
2008-01-17
Authors:
Ananthamurthy, Koteshwara; Kathir, Karuppanan; Kight, Alicia; Goforth, Robyn; Henry, Ralph; Suresh Kumar, Thallapuranam
Citation:

Citation: Ananthamurthy, Koteshwara; Kathir, Karuppanan Muthusamy; Kight, Alicia; Goforth, Robyn; Henry, Ralph; Suresh Kumar, Thallapuranam Krishnaswamy. "1H, 13C and 15N resonance assignments of the C-terminal domain of the 43 kDa subunit of the chloroplast signal recognition particle"  Biomol. NMR Assignments 2, 37-39 (2008).
PubMed: 19636919

Assembly members:

Assembly members:
C-Terminal_chromo_domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-Terminal_chromo_domain: GSQVFEYAEVDEIVEKRGKG KDVEYLVRWKDGGDCEWVKG VHVAEDVAKDYEDGLEYAVA ESVIGKRVGDDGKTIEYLVK WTDMSDATWEPQDNVDSTLV LLYQQQQPMNE

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts109
1H chemical shifts712

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal chromo domain1

Entities:

Entity 1, C-terminal chromo domain 111 residues - Formula weight is not available

1   GLYSERGLNVALPHEGLUTYRALAGLUVAL
2   ASPGLUILEVALGLULYSARGGLYLYSGLY
3   LYSASPVALGLUTYRLEUVALARGTRPLYS
4   ASPGLYGLYASPCYSGLUTRPVALLYSGLY
5   VALHISVALALAGLUASPVALALALYSASP
6   TYRGLUASPGLYLEUGLUTYRALAVALALA
7   GLUSERVALILEGLYLYSARGVALGLYASP
8   ASPGLYLYSTHRILEGLUTYRLEUVALLYS
9   TRPTHRASPMETSERASPALATHRTRPGLU
10   PROGLNASPASNVALASPSERTHRLEUVAL
11   LEULEUTYRGLNGLNGLNGLNPROMETASN
12   GLU

Samples:

sample_1: C-Terminal chromo domain, [U-98% 13C; U-98% 15N], 0.7 ± 0.05 mM; sodium chloride 150 mM

sample_2: C-Terminal chromo domain, [U-99% 15N], 0.7 ± 0.05 mM; sodium chloride 150 mM

sample_3: C-Terminal chromo domain, [U-13C], 0.7 ± 0.05 mM; sodium chloride 150 mM

sample_4: C-Terminal chromo domain 1 ± 0.05 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin, Goddard - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks