BMRB Entry 7223
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7223
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Aramini, J.; Ho, C.; Yin, C.; Cunningham, K.; Janjua, H.; Li, M.-C.; Xiao, R.; Acton, T.; Montelione, G.. "Solution NMR structure of the C-terminal domain of the interferon
alpha-inducible ISG15 protein from Homo sapiens. Northeast Structural Genomics
target HR2873B." .
Assembly members:
Interferon-induced 17 kDa protein, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Interferon-induced 17 kDa protein: MDEPLSILVRNNKGRSSTYE
VRLTQTVAHLKQQVSGLEGV
QDDLFWLTFEGKPLEDQLPL
GEYGLKPLSTVFMNLRLRGG
LEHHHHHH
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 375 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 605 |
| coupling constants | 57 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Interferon-induced 17 kDa protein | 1 |
Entities:
Entity 1, Interferon-induced 17 kDa protein 88 residues - Formula weight is not available
| 1 | MET | ASP | GLU | PRO | LEU | SER | ILE | LEU | VAL | ARG | ||||
| 2 | ASN | ASN | LYS | GLY | ARG | SER | SER | THR | TYR | GLU | ||||
| 3 | VAL | ARG | LEU | THR | GLN | THR | VAL | ALA | HIS | LEU | ||||
| 4 | LYS | GLN | GLN | VAL | SER | GLY | LEU | GLU | GLY | VAL | ||||
| 5 | GLN | ASP | ASP | LEU | PHE | TRP | LEU | THR | PHE | GLU | ||||
| 6 | GLY | LYS | PRO | LEU | GLU | ASP | GLN | LEU | PRO | LEU | ||||
| 7 | GLY | GLU | TYR | GLY | LEU | LYS | PRO | LEU | SER | THR | ||||
| 8 | VAL | PHE | MET | ASN | LEU | ARG | LEU | ARG | GLY | GLY | ||||
| 9 | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Interferon-induced 17 kDa protein, [U-13C; U-15N], 0.81 mM; ammonium citrate 50 mM; CaCl2 5 mM; NaN3 0.02%; D2O 5%; H2O 95%
sample_2: Interferon-induced 17 kDa protein, [U-5% 13C; U-15N], 0.9 mM; ammonium citrate 50 mM; CaCl2 5 mM; NaN3 0.02%; D2O 5%; H2O 95%
sample_cond_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| high resolution 2D CH-HSQC (for stereospecific assignment of Val/Leu methyls) | not available | not available | not available |
| 2D 15N 1H heteronuclear NOE | not available | not available | not available |
| 3D TR backbone expts | not available | not available | not available |
| 3D HCCH-COSY | not available | not available | not available |
| 3D HCCH-TOCSYs | not available | not available | not available |
Software:
xwinnmr v3.5pl6 - collection
VNMR v6.1C - collection
AutoAssign v2.2.1 - data analysis
SPARKY v3.110 - data analysis
AutoStruct v2.1.1 - refinement
X-PLOR NIH v2.11.2 - refinement
NMRPipe v2.3 - processing
PDBStat v4.1 - data analysis
PSVS v1.3 - data analysis
CNS v1.1 - refinement
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 5658 |
| PDB | |
| DBJ | BAJ20347 |
| GB | AAA36038 AAA36128 AAH09507 AAN86983 AAP35961 |
| REF | NP_005092 XP_003806031 XP_004024480 XP_009452953 XP_520842 |
| SP | P05161 |
| AlphaFold | P05161 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks