BMRB Entry 5658

Name: Sequence specific backbone assignment of 13C, 2H, 15N labeled ISG15
Published: 2003-02-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5658

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence specific backbone assignment of 13C, 2H, 15N labeled ISG15

Deposition date:

2003-01-13

Original release date:

2003-02-25

Authors:

Yin, Cuifeng; Cort, John; Swapna, G.; Krug, Robert; Kennedy, Michael; Montelione, Gaetano

Citation:

Citation: Yin, Cuifeng; Aramini, J.; Ma, L.-C.; Cort, John; Swapna, G.; Krug, Robert; Montelione, Gaetano. "Backbone and Ile-delta1, Leu, Val methyl 1H, 13C, and 15N NMR chemical shift assignments for human interferon-stimulated gene 15 protein" Biomol. NMR Assign. ., .-..

Assembly members:

Assembly members:
ISG15, polymer, 164 residues, 18055.6 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKM596

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ISG15: SHHHHHHMGWDLTVKMLAGN EFQVSLSSSMSVSELKAQIT QKIGVHAFQQRLAVHPSGVA LQDRVPLASQGLGPGSTVLL VVDKCDEPLSILVRNNKGRS STYEVRLTQTVAHLKQQVSG LEGVQDDLFWLTFEGKPLED QLPLGEYGLKPLSTVFMNLR LRGG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
1H chemical shifts	399
13C chemical shifts	530
15N chemical shifts	162

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ISG15 monomer	1

Entities:

Entity 1, ISG15 monomer 164 residues - 18055.6 Da.

1

SER

HIS

MET

GLY

TRP

2

ASP

LEU

THR

VAL

LYS

MET

LEU

ALA

GLY

ASN

3

GLU

PHE

GLN

VAL

SER

LEU

SER

MET

4

SER

VAL

SER

GLU

LEU

LYS

ALA

GLN

ILE

THR

5

GLN

LYS

ILE

GLY

VAL

HIS

ALA

PHE

GLN

6

ARG

LEU

ALA

VAL

HIS

PRO

SER

GLY

VAL

ALA

7

LEU

GLN

ASP

ARG

VAL

PRO

LEU

ALA

SER

GLN

8

GLY

LEU

GLY

PRO

GLY

SER

THR

VAL

LEU

9

VAL

ASP

LYS

CYS

ASP

GLU

PRO

LEU

SER

10

ILE

LEU

VAL

ARG

ASN

LYS

GLY

ARG

SER

11

SER

THR

TYR

GLU

VAL

ARG

LEU

THR

GLN

THR

12

VAL

ALA

HIS

LEU

LYS

GLN

VAL

SER

GLY

13

LEU

GLU

GLY

VAL

GLN

ASP

LEU

PHE

TRP

14

LEU

THR

PHE

GLU

GLY

LYS

PRO

LEU

GLU

ASP

15

GLN

LEU

PRO

LEU

GLY

GLU

TYR

GLY

LEU

LYS

16

PRO

LEU

SER

THR

VAL

PHE

MET

ASN

LEU

ARG

17

LEU

ARG

GLY

Samples:

sample_1: Interferon-Stimulated gene 15 product, [U-70% 2H; U-100% 13C; U-100% 15N], 0.7 mM; ammonium citrate 50 mM; CaCl2 5 mM; DTT 10 mM

condition_1: pH: 6.5; temperature: 291 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	sample_1	not available	condition_1
HNCA	sample_1	not available	condition_1
HN(CO)CA	sample_1	not available	condition_1
HNCO	sample_1	not available	condition_1
HN(CA)CO	sample_1	not available	condition_1
HNCACB	sample_1	not available	condition_1
HN(CO)CACB	sample_1	not available	condition_1
HSQC	sample_1	not available	condition_1

Software:

AUTOASSIGN v1.10.0 - automated peak assignments

NMR spectrometers:

Varian INOVA 750 MHz

PDB	1Z2M 3PSE 3R66 3RT3 3SDL
DBJ	BAJ20347
GB	AAA36038 AAA36128 AAH09507 AAN86983 AAP35961
REF	NP_005092 XP_003806031 XP_009452953 XP_520842
SP	P05161
AlphaFold	P05161