BMRB Entry 7190

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7190

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Title:
The structure and function of a novel two-site calcium-binding fragment of calmodulin
Deposition date:
2006-06-22
Original release date:
2007-09-24
Authors:
Lakowski, Ted; Lee, Greg; Reid, Ronald; McIntosh, Lawrence
Citation:

Citation: Lakowski, Ted; Lee, Greg; Okon, M.; Reid, Ronald; McIntosh, Lawrence. "Calcium-induced folding of a fragment of calmodulin composed of EF-hands 2 and 3"  Protein Sci. 16, 1119-1132 (2007).
PubMed: 17473011

Assembly members:

Assembly members:
CaM2/3, polymer, 68 residues, 7701 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaM2/3: AELQDMINEVDADGNGTIDF PEFLTMMARKMKDTDSEEEI REAFRVFDKDGNGYISAAEL RHVMTNLG

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts70
1H chemical shifts449
heteronuclear NOE values64
T1 relaxation values64
T2 relaxation values64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CaM2/31
2CALCIUM (II) ION, 12
3CALCIUM (II) ION, 22

Entities:

Entity 1, CaM2/3 68 residues - 7701 Da.

1   ALAGLULEUGLNASPMETILEASNGLUVAL
2   ASPALAASPGLYASNGLYTHRILEASPPHE
3   PROGLUPHELEUTHRMETMETALAARGLYS
4   METLYSASPTHRASPSERGLUGLUGLUILE
5   ARGGLUALAPHEARGVALPHEASPLYSASP
6   GLYASNGLYTYRILESERALAALAGLULEU
7   ARGHISVALMETTHRASNLEUGLY

Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 11440 16994 19376 25344 6789 7016 7017
PDB 1CMF 1CMG 1YRT 1YRU 2COL 2HF5 2KZ2 2RRT 4BYA
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566 CAB65357 CCJ31183
GB AAB87698 AAC23998 AAC23999 AAH07965 AAH10730
REF XP_001687795 XP_001869425 XP_002404770 XP_004322571 XP_004610315
TPG DAA24989 DAA24990

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