BMRB Entry 7125

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7125
MolProbity Validation Chart

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Title:
Chemical Shifts Assignments of Human Adult Hemoglobin in the Carbonmonoxy Form
Deposition date:
2006-05-22
Original release date:
2006-11-13
Authors:
Fan, Jing-song; Yang, Daiwen
Citation:

Citation: Xu, Y.; Zheng, Y.; Fan, J.S.; Yang, D.. "A new strategy for structure determination of large proteins in solution without deuteration"  Nature Methods 3, 931-937 (2006).
PubMed: 17060917

Assembly members:

Assembly members:
alpha chain, polymer, 141 residues, Formula weight is not available
beta chain, polymer, 146 residues, Formula weight is not available
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha chain: VLSPADKTNVKAAWGKVGAH AGEYGAEALERMFLSFPTTK TYFPHFDLSHGSAQVKGHGK KVADALTNAVAHVDDMPNAL SALSDLHAHKLRVDPVNFKL LSHCLLVTLAAHLPAEFTPA VHASLDKFLASVSTVLTSKY R
beta chain: VHLTPEEKSAVTALWGKVNV DEVGGEALGRLLVVYPWTQR FFESFGDLSTPDAVMGNPKV KAHGKKVLGAFSDGLAHLDN LKGTFATLSELHCDKLHVDP ENFRLLGNVLVCVLAHHFGK EFTPPVQAAYQKVVAGVANA LAHKYH

Data sets:
Data typeCount
13C chemical shifts856
15N chemical shifts270
1H chemical shifts1635

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1globin subunit alpha, 11
2$globin_subunit_alpha.
3.yes
4.yes
5.yes
6.yes
7.yes
8.yes

Entities:

Entity 1, globin subunit alpha, 1 141 residues - Formula weight is not available

1   VALLEUSERPROALAASPLYSTHRASNVAL
2   LYSALAALATRPGLYLYSVALGLYALAHIS
3   ALAGLYGLUTYRGLYALAGLUALALEUGLU
4   ARGMETPHELEUSERPHEPROTHRTHRLYS
5   THRTYRPHEPROHISPHEASPLEUSERHIS
6   GLYSERALAGLNVALLYSGLYHISGLYLYS
7   LYSVALALAASPALALEUTHRASNALAVAL
8   ALAHISVALASPASPMETPROASNALALEU
9   SERALALEUSERASPLEUHISALAHISLYS
10   LEUARGVALASPPROVALASNPHELYSLEU
11   LEUSERHISCYSLEULEUVALTHRLEUALA
12   ALAHISLEUPROALAGLUPHETHRPROALA
13   VALHISALASERLEUASPLYSPHELEUALA
14   SERVALSERTHRVALLEUTHRSERLYSTYR
15   ARG

Entity ., $globin_subunit_alpha

Entity yes, .

Samples:

sample_1: HbCO A, [U-13C; U-15N], 1.2 mM; HbCO A, [U-13C; U-15N], 1.0 mM

conditions_1: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
4D 13C, 15N-edited NOESYsample_1not availableconditions_1
4D 13C, 13C-edited NOESYsample_1not availableconditions_1
3D MQ-CCH-TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker avance 800 MHz
  • Bruker avance 500 MHz

Related Database Links:

BMRB 1053 1102 2006 2708 2710 5856 6230 6683 908
PDB
DBJ BAG34767
EMBL CAA23756 CAA23758 CAA23759 CAA43421 CAG38767
GB AAA16334 AAA21100 AAA21101 AAA21102 AAA21103
PRF 0404170B 0907233B
REF NP_000509 XP_002822173 XP_003819077 XP_004050595 XP_004090697
SP P02024 P02025 P02032 P68871 P68872
AlphaFold P02024 P02025 P02032 P68871 P68872

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks