BMRB Entry 5856
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5856
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Citation: Lukin, Jonathan; Kontaxis, Georg; Simplaceanu, Virgil; Yuan, Yue; Bax, Ad; Ho, Chien. "Letter to the Editor: Backbone Resonance Assignments of Human Adult Hemoglobin
in the Carbonmonoxy Form" J. Biomol. NMR 28, 203-204 (2004).
PubMed: 14755170
Assembly members:
globin subunit alpha, polymer, 141 residues, Formula weight is not available
globin subunit beta, polymer, 146 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
CMO, non-polymer, 28.010 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHE2
Entity Sequences (FASTA):
globin subunit alpha: VLSPADKTNVKAAWGKVGAH
AGEYGAEALERMFLSFPTTK
TYFPHFDLSHGSAQVKGHGK
KVADALTNAVAHVDDMPNAL
SALSDLHAHKLRVDPVNFKL
LSHCLLVTLAAHLPAEFTPA
VHASLDKFLASVSTVLTSKY
R
globin subunit beta: VHLTPEEKSAVTALWGKVNV
DEVGGEALGRLLVVYPWTQR
FFESFGDLSTPDAVMGNPKV
KAHGKKVLGAFSDGLAHLDN
LKGTFATLSELHCDKLHVDP
ENFRLLGNVLVCVLAHHFGK
EFTPPVQAAYQKVVAGVANA
LAHKYH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 749 |
| 15N chemical shifts | 263 |
| 1H chemical shifts | 263 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | globin subunit alpha, one | 1 |
| 2 | globin subunit alpha, two | 1 |
| 3 | globin subunit beta, one | 2 |
| 4 | globin subunit beta, two | 2 |
| 5 | heme unit, one | 3 |
| 6 | heme unit, two | 3 |
| 7 | carbon monoxide, one | 4 |
| 8 | carbon monoxide, two | 4 |
Entities:
Entity 1, globin subunit alpha, one 141 residues - Formula weight is not available
| 1 | VAL | LEU | SER | PRO | ALA | ASP | LYS | THR | ASN | VAL | ||||
| 2 | LYS | ALA | ALA | TRP | GLY | LYS | VAL | GLY | ALA | HIS | ||||
| 3 | ALA | GLY | GLU | TYR | GLY | ALA | GLU | ALA | LEU | GLU | ||||
| 4 | ARG | MET | PHE | LEU | SER | PHE | PRO | THR | THR | LYS | ||||
| 5 | THR | TYR | PHE | PRO | HIS | PHE | ASP | LEU | SER | HIS | ||||
| 6 | GLY | SER | ALA | GLN | VAL | LYS | GLY | HIS | GLY | LYS | ||||
| 7 | LYS | VAL | ALA | ASP | ALA | LEU | THR | ASN | ALA | VAL | ||||
| 8 | ALA | HIS | VAL | ASP | ASP | MET | PRO | ASN | ALA | LEU | ||||
| 9 | SER | ALA | LEU | SER | ASP | LEU | HIS | ALA | HIS | LYS | ||||
| 10 | LEU | ARG | VAL | ASP | PRO | VAL | ASN | PHE | LYS | LEU | ||||
| 11 | LEU | SER | HIS | CYS | LEU | LEU | VAL | THR | LEU | ALA | ||||
| 12 | ALA | HIS | LEU | PRO | ALA | GLU | PHE | THR | PRO | ALA | ||||
| 13 | VAL | HIS | ALA | SER | LEU | ASP | LYS | PHE | LEU | ALA | ||||
| 14 | SER | VAL | SER | THR | VAL | LEU | THR | SER | LYS | TYR | ||||
| 15 | ARG |
Entity 2, globin subunit beta, one 146 residues - Formula weight is not available
| 1 | VAL | HIS | LEU | THR | PRO | GLU | GLU | LYS | SER | ALA | ||||
| 2 | VAL | THR | ALA | LEU | TRP | GLY | LYS | VAL | ASN | VAL | ||||
| 3 | ASP | GLU | VAL | GLY | GLY | GLU | ALA | LEU | GLY | ARG | ||||
| 4 | LEU | LEU | VAL | VAL | TYR | PRO | TRP | THR | GLN | ARG | ||||
| 5 | PHE | PHE | GLU | SER | PHE | GLY | ASP | LEU | SER | THR | ||||
| 6 | PRO | ASP | ALA | VAL | MET | GLY | ASN | PRO | LYS | VAL | ||||
| 7 | LYS | ALA | HIS | GLY | LYS | LYS | VAL | LEU | GLY | ALA | ||||
| 8 | PHE | SER | ASP | GLY | LEU | ALA | HIS | LEU | ASP | ASN | ||||
| 9 | LEU | LYS | GLY | THR | PHE | ALA | THR | LEU | SER | GLU | ||||
| 10 | LEU | HIS | CYS | ASP | LYS | LEU | HIS | VAL | ASP | PRO | ||||
| 11 | GLU | ASN | PHE | ARG | LEU | LEU | GLY | ASN | VAL | LEU | ||||
| 12 | VAL | CYS | VAL | LEU | ALA | HIS | HIS | PHE | GLY | LYS | ||||
| 13 | GLU | PHE | THR | PRO | PRO | VAL | GLN | ALA | ALA | TYR | ||||
| 14 | GLN | LYS | VAL | VAL | ALA | GLY | VAL | ALA | ASN | ALA | ||||
| 15 | LEU | ALA | HIS | LYS | TYR | HIS |
Entity 3, heme unit, one - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Entity 4, carbon monoxide, one - C O - 28.010 Da.
| 1 | CMO |
Samples:
sample_one: globin subunit alpha, [U-2H; U-13C; U-15N], 1.0 mM; globin subunit beta, [U-2H; U-13C; U-15N], 1.0 mM; Na phosphate 0.1 M; H2O 90%; D2O 10%
sample_conditions: pH: 7.0; temperature: 302.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks