BMRB Entry 7097

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PDB ID: 2glo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7097
MolProbity Validation Chart

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Title:
DNA recognition by the Brinker nuclear repressor - an extreme case of the coupling between binding and folding
Deposition date:
2006-05-05
Original release date:
2006-09-22
Authors:
Cordier, F.; Hartmann, B.; Rogowski, M.; Affolter, M.; Grzesiek, S.
Citation:

Citation: Cordier, F.; Hartmann, B.; Rogowski, M.; Affolter, M.; Grzesiek, S.. "DNA recognition by the Brinker repressor - an extreme case of coupling between binding and folding"  J. Mol. Biol. 361, 659-672 (2006).
PubMed: 16876822

Assembly members:

Assembly members:
brinker CG9653-PA, polymer, 59 residues, Formula weight is not available
5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3', polymer, 12 residues, Formula weight is not available
5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3', polymer, 12 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
brinker CG9653-PA: GSRRIFTPHFKLQVLESYRN DNDCKGNQRATARKYNIHRR QIQKWLQCESNLRSSVANN
5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3': TGAGGCGTCAAC
5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3': GTTGACGCCTCA

Data typeCount
13C chemical shifts255
15N chemical shifts75
1H chemical shifts582

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1brinker CG9653-PA1
25'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3'2
35'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3'3

Entities:

Entity 1, brinker CG9653-PA 59 residues - Formula weight is not available

1   GLYSERARGARGILEPHETHRPROHISPHE
2   LYSLEUGLNVALLEUGLUSERTYRARGASN
3   ASPASNASPCYSLYSGLYASNGLNARGALA
4   THRALAARGLYSTYRASNILEHISARGARG
5   GLNILEGLNLYSTRPLEUGLNCYSGLUSER
6   ASNLEUARGSERSERVALALAASNASN

Entity 2, 5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' 12 residues - Formula weight is not available

1   DTDGDADGDGDCDGDTDCDA
2   DADC

Entity 3, 5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' 12 residues - Formula weight is not available

1   DGDTDTDGDADCDGDCDCDT
2   DCDA

Related Database Links:

BMRB 7134
PDB 2GLO
DBJ BAA76710
GB AAD22080 AAF46251 ACD81876 ACK77656 EDV34122
REF NP_511069 XP_001963673 XP_001978531 XP_002022676 XP_002043219

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