BMRB Entry 7134

Name: DNA recognition by the Brinker nuclear repressor - an extreme case of the coupling between binding and folding
Published: 2006-10-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7134

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DNA recognition by the Brinker nuclear repressor - an extreme case of the coupling between binding and folding

Deposition date:

2006-05-23

Original release date:

2006-10-19

Authors:

Cordier, F.; Hartmann, B.; Rogowski, M.; Affolter, M.; Grzesiek, S.

Citation:

Citation: Cordier, F.; Hartmann, B.; Rogowski, M.; Affolter, M.; Grzesiek, S.. "DNA recognition by the Brinker repressor - an extreme case of coupling between binding and folding" J. Mol. Biol. 361, 659-672 (2006).
PubMed: 16876822

Assembly members:

Assembly members:
brinker CG9653-PA, polymer, 59 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
brinker CG9653-PA: GSRRIFTPHFKLQVLESYRN DNDCKGNQRATARKYNIHRR QIQKWLQCESNLRSSVANN

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	112
15N chemical shifts	55
1H chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	brinker CG9653-PA	1

Entities:

Entity 1, brinker CG9653-PA 59 residues - Formula weight is not available

1

GLY

SER

ARG

ILE

PHE

THR

PRO

HIS

PHE

2

LYS

LEU

GLN

VAL

LEU

GLU

SER

TYR

ARG

ASN

3

ASP

ASN

ASP

CYS

LYS

GLY

ASN

GLN

ARG

ALA

4

THR

ALA

ARG

LYS

TYR

ASN

ILE

HIS

ARG

5

GLN

ILE

GLN

LYS

TRP

LEU

GLN

CYS

GLU

SER

6

ASN

LEU

ARG

SER

VAL

ALA

ASN

Samples:

sample_1: brinker CG9653-PA, [U-13C; U-15N], 1 mM; NaCl 10 mM; potassium phosphate 20 mM; DTT 2 mM; NaN3 0.02%; H2O 95%; D2O 5%

sample_cond_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	sample_cond_1
3D 13C-separated NOESY	sample_1	not available	sample_cond_1
3D dq-HNHA	sample_1	not available	sample_cond_1
3D dq-HAHB	sample_1	not available	sample_cond_1
2D HNCG	sample_1	not available	sample_cond_1
2D HN(CO)CG	sample_1	not available	sample_cond_1
2D 13C-selected/12C,14N-selected NOESY	sample_1	not available	sample_cond_1
DSSE	sample_1	not available	sample_cond_1
J-modulated-13C-HSQC	sample_1	not available	sample_cond_1

Software:

xwinnmr v2.6 - collection

NMRPipe v2.2 - processing

PIPP v4.3.2 - data analysis

NMR spectrometers:

Bruker DRX 800 MHz

BMRB	7097
PDB	2GLO
DBJ	BAA76710
GB	AAD22080 AAF46251 ACD81876 ACK77656 EDV34122
REF	NP_511069 XP_001963673 XP_001978531 XP_002022676 XP_002043219