BMRB Entry 7097
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7097
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Cordier, F.; Hartmann, B.; Rogowski, M.; Affolter, M.; Grzesiek, S.. "DNA recognition by the Brinker repressor - an extreme case of coupling between
binding and folding" J. Mol. Biol. 361, 659-672 (2006).
PubMed: 16876822
Assembly members:
brinker CG9653-PA, polymer, 59 residues, Formula weight is not available
5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3', polymer, 12 residues, Formula weight is not available
5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3', polymer, 12 residues, Formula weight is not available
Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b(+)
Entity Sequences (FASTA):
brinker CG9653-PA: GSRRIFTPHFKLQVLESYRN
DNDCKGNQRATARKYNIHRR
QIQKWLQCESNLRSSVANN
5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3': TGAGGCGTCAAC
5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3': GTTGACGCCTCA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 255 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 582 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | brinker CG9653-PA | 1 |
| 2 | 5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' | 2 |
| 3 | 5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' | 3 |
Entities:
Entity 1, brinker CG9653-PA 59 residues - Formula weight is not available
| 1 | GLY | SER | ARG | ARG | ILE | PHE | THR | PRO | HIS | PHE | ||||
| 2 | LYS | LEU | GLN | VAL | LEU | GLU | SER | TYR | ARG | ASN | ||||
| 3 | ASP | ASN | ASP | CYS | LYS | GLY | ASN | GLN | ARG | ALA | ||||
| 4 | THR | ALA | ARG | LYS | TYR | ASN | ILE | HIS | ARG | ARG | ||||
| 5 | GLN | ILE | GLN | LYS | TRP | LEU | GLN | CYS | GLU | SER | ||||
| 6 | ASN | LEU | ARG | SER | SER | VAL | ALA | ASN | ASN |
Entity 2, 5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' 12 residues - Formula weight is not available
| 1 | DT | DG | DA | DG | DG | DC | DG | DT | DC | DA | ||||
| 2 | DA | DC |
Entity 3, 5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' 12 residues - Formula weight is not available
| 1 | DG | DT | DT | DG | DA | DC | DG | DC | DC | DT | ||||
| 2 | DC | DA |
Samples:
sample_1: brinker CG9653-PA, [U-15N], 1 mM; 5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' 1 mM; 5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' 1 mM; NaCl 10 mM; potassium phosphate 5 mM; TCEP 5 mM; NaN3 0.02%; H2O 95%; D2O 5%
sample_2: brinker CG9653-PA, [U-13C; U-15N], 1 mM; 5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' 1 mM; 5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' 1 mM; NaCl 10 mM; potassium phosphate 5 mM; TCEP 5 mM; NaN3 0.02%; H2O 95%; D2O 5%
sample_3: brinker CG9653-PA, [U-13C; U-15N], 0.35 mM; 5'-D(*TP*GP*AP*GP*GP*CP*GP*TP*CP*AP*AP*C)-3' 0.35 mM; 5'-D(*GP*TP*TP*GP*AP*CP*GP*CP*CP*TP*CP*A)-3' 0.35 mM; NaCl 10 mM; potassium phosphate 20 mM; DTT 2 mM; NaN3 0.02%; Pf1-phages 25 mg/mL; H2O 95%; D2O 5%
sample_cond_1: ionic strength: 10 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D dq-HNHA | not available | not available | not available |
| 3D dq-HAHB | not available | not available | not available |
| 2D HNCG | not available | not available | not available |
| 2D HN(CO)CG | not available | not available | not available |
| 2D 13C-selected/12C,14N-selected NOESY | not available | not available | not available |
| DSSE | not available | not available | not available |
| J-modulated-13C-HSQC | not available | not available | not available |
Software:
xwinnmr v2.6 - collection
NMRPipe v2.2 - processing
PIPP v4.3.2 - data analysis
HADDOCK v1.2 - refinement
NMR spectrometers:
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 7134 |
| PDB | |
| DBJ | BAA76710 |
| GB | AAD22080 AAF46251 ACD81876 ACK77656 EDV34122 |
| REF | NP_511069 XP_001963673 XP_001978531 XP_002022676 XP_002043219 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks