BMRB Entry 6202

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6202

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the 1H, 15N and 13C resonances of the Class II E2 Conjugating Enzyme, Ubc1

Deposition date:

2004-05-07

Original release date:

2004-12-16

Authors:

Merkley, Nadine; Shaw, Gary

Citation:

Citation: Merkley, Nadine; Shaw, Gary. "Letter to the Editor: Assignment of the 1H, 13C, and 15N resonances of the class II E2 conjugating enzyme, Ubc1" J. Biomol NMR 30, 369-370 (2004).

Assembly members:

Assembly members:
yeast Ubc1, polymer, 215 residues, 24162 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yeast Ubc1: SSRAKRIMKEIQAVKDDPAA HITLEFVSESDIHHLKGTFL GPPGTPYEGGKFVVDIEVPM EYPFKPPKMQFDTKVYHPNI SSVTGAICLDILRNAWSPVI TLKSALISLQALLQSPEPND PQDAEVAQHYLRDRESFNKT AALWTRLYASETSNGQKGNV EESDLYGIDHDLIDEFESQG FEKDKIVEVLRRLGVKSLDP NDNNTANRIIEELLK

Data sets:

assigned_chemical_shifts
- Ubc1_chemical_shifts

Data type	Count
1H chemical shifts	1307
13C chemical shifts	804
15N chemical shifts	190

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	yUbc1(K93R)	1

Entities:

Entity 1, yUbc1(K93R) 215 residues - 24162 Da.

1

SER

ARG

ALA

LYS

ARG

ILE

MET

LYS

GLU

2

ILE

GLN

ALA

VAL

LYS

ASP

PRO

ALA

3

HIS

ILE

THR

LEU

GLU

PHE

VAL

SER

GLU

SER

4

ASP

ILE

HIS

LEU

LYS

GLY

THR

PHE

LEU

5

GLY

PRO

GLY

THR

PRO

TYR

GLU

GLY

6

LYS

PHE

VAL

ASP

ILE

GLU

VAL

PRO

MET

7

GLU

TYR

PRO

PHE

LYS

PRO

LYS

MET

GLN

8

PHE

ASP

THR

LYS

VAL

TYR

HIS

PRO

ASN

ILE

9

SER

VAL

THR

GLY

ALA

ILE

CYS

LEU

ASP

10

ILE

LEU

ARG

ASN

ALA

TRP

SER

PRO

VAL

ILE

11

THR

LEU

LYS

SER

ALA

LEU

ILE

SER

LEU

GLN

12

ALA

LEU

GLN

SER

PRO

GLU

PRO

ASN

ASP

13

PRO

GLN

ASP

ALA

GLU

VAL

ALA

GLN

HIS

TYR

14

LEU

ARG

ASP

ARG

GLU

SER

PHE

ASN

LYS

THR

15

ALA

LEU

TRP

THR

ARG

LEU

TYR

ALA

SER

16

GLU

THR

SER

ASN

GLY

GLN

LYS

GLY

ASN

VAL

17

GLU

SER

ASP

LEU

TYR

GLY

ILE

ASP

HIS

18

ASP

LEU

ILE

ASP

GLU

PHE

GLU

SER

GLN

GLY

19

PHE

GLU

LYS

ASP

LYS

ILE

VAL

GLU

VAL

LEU

20

ARG

LEU

GLY

VAL

LYS

SER

LEU

ASP

PRO

21

ASN

ASP

ASN

THR

ALA

ASN

ARG

ILE

22

GLU

LEU

LYS

Samples:

sample_1: yeast Ubc1, [U-15N; U-13C], 0.48 mM; Tris 25 mM; EDTA 1 mM; DTT 1 mM; NaCl 150 mM

Ex-cond_1: pH: 7.5; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-13C HSQC	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNCO	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HC(CO)NH	not available	not available	not available
C(CO)NH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available

Software:

NMRPipe -

Pipp -

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	4567
PDB	1FXT 1FZY 1TTE
DBJ	GAA22407
EMBL	CAA39812 CAA86682 CAY78679
GB	AAS56001 AHY75163 EDN60515 EDV08135 EDZ73074
REF	NP_010462
SP	P21734
TPG	DAA12019
AlphaFold	P21734