BMRB Entry 6100

Title:
Backbone 1H, 13C and 15N chemical shift assignents of the 18.5kDa isoform of murine myelin basic protein (MBP)
Deposition date:
2004-02-12
Original release date:
2007-10-19
Authors:
Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George
Citation:

Citation: Libich, David; Robertson, Valerie; Monette, Martine; Harauz, George. "Letter to the Editor: Backbone resonance assignments of the 18.5kDa isoform of murine myelin basic protein (MBP)"  J. Biomol. NMR 29, 545-546 (2004).
PubMed: 15243191

Assembly members:

Assembly members:
myelin basic protein, polymer, 176 residues, 19421.5 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet22b+

Data sets:
Data typeCount
13C chemical shifts436
15N chemical shifts154
1H chemical shifts516

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rmMBP1

Entities:

Entity 1, rmMBP 176 residues - 19421.5 Da.

1   ALASERGLNLYSARGPROSERGLNARGSER
2   LYSTYRLEUALATHRALASERTHRMETASP
3   HISALAARGHISGLYPHELEUPROARGHIS
4   ARGASPTHRGLYILELEUASPSERILEGLY
5   ARGPHEPHESERGLYASPARGGLYALAPRO
6   LYSARGGLYSERGLYLYSASPSERHISTHR
7   ARGTHRTHRHISTYRGLYSERLEUPROGLN
8   LYSSERGLNHISGLYARGTHRGLNASPGLU
9   ASNPROVALVALHISPHEPHELYSASNILE
10   VALTHRPROARGTHRPROPROPROSERGLN
11   GLYLYSGLYARGGLYLEUSERLEUSERARG
12   PHESERTRPGLYALAGLUGLYGLNARGPRO
13   GLYPHEGLYTYRGLYGLYARGALASERASP
14   TYRLYSSERALAHISLYSGLYPHELYSGLY
15   ALATYRASPALAGLNGLYTHRLEUSERLYS
16   ILEPHELYSLEUGLYGLYARGASPSERARG
17   SERGLYSERPROMETALAARGARGLEUGLU
18   HISHISHISHISHISHIS

Samples:

sample_1: myelin basic protein, [U-15N; U-13C], 2 mM; 2,2,2-trifluoroethanol-d2, [2H], 30%

sample_cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N-HSQCsample_1not availablesample_cond_1
CBCANHsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HCC(CO)NHsample_1not availablesample_cond_1
HNCOsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AV 600 MHz

Related Database Links:

BMRB 15131
GB AAA37720 AAA39496 AAA39501 EDL09385
REF NP_001020426 NP_001020463 NP_034907 XP_006526519
SP P04370
AlphaFold P04370

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks