BMRB Entry 5917

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5917
MolProbity Validation Chart

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Title:
Backbone 1HN, 13C, 15N Chemical Shift Assignments for human eIF2alpha
Deposition date:
2003-08-21
Original release date:
2004-02-13
Authors:
Ito, Takuhiro
Citation:

Citation: Ito, Takuhiro; Wagner, Gerhard. "Using condon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of Human eIF2a"  J. Biomol. NMR 28, 357-367 (2004).

Assembly members:

Assembly members:
eIF2alpha, polymer, 308 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF2alpha: MSCRFYQHKFPEVEDVVMVN VRSIQEMGAYVSLLEYNNIE GMIHLSELSRRRIRSINKLI RIGRNECVKVIRVDKEKGYI DLSKRRVSPEEAIKCEDKFT KSKTVYSILRHVAEVLEYTK DEQLESLFQRTAWVFDDKYK RPGYGAYDAFKHAVSDPSIL DSLDLNEDEREVLINNINRR LTPQAVKIRADIEVACYGYE GIDAVKEALRAGLNCSTENM PIKINLIAPPRYVMTTTTLE RTEGLSVLSQAMAVIKEKIE EKRGVFNVQMEPKVVTDTDE TELARQMERLERENAEVDGD LEHHHHHH

Data sets:
Data typeCount
1H chemical shifts278
13C chemical shifts566
15N chemical shifts278

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1heIF2alpha monomer1

Entities:

Entity 1, heIF2alpha monomer 308 residues - Formula weight is not available

1   METSERCYSARGPHETYRGLNHISLYSPHE
2   PROGLUVALGLUASPVALVALMETVALASN
3   VALARGSERILEGLNGLUMETGLYALATYR
4   VALSERLEULEUGLUTYRASNASNILEGLU
5   GLYMETILEHISLEUSERGLULEUSERARG
6   ARGARGILEARGSERILEASNLYSLEUILE
7   ARGILEGLYARGASNGLUCYSVALLYSVAL
8   ILEARGVALASPLYSGLULYSGLYTYRILE
9   ASPLEUSERLYSARGARGVALSERPROGLU
10   GLUALAILELYSCYSGLUASPLYSPHETHR
11   LYSSERLYSTHRVALTYRSERILELEUARG
12   HISVALALAGLUVALLEUGLUTYRTHRLYS
13   ASPGLUGLNLEUGLUSERLEUPHEGLNARG
14   THRALATRPVALPHEASPASPLYSTYRLYS
15   ARGPROGLYTYRGLYALATYRASPALAPHE
16   LYSHISALAVALSERASPPROSERILELEU
17   ASPSERLEUASPLEUASNGLUASPGLUARG
18   GLUVALLEUILEASNASNILEASNARGARG
19   LEUTHRPROGLNALAVALLYSILEARGALA
20   ASPILEGLUVALALACYSTYRGLYTYRGLU
21   GLYILEASPALAVALLYSGLUALALEUARG
22   ALAGLYLEUASNCYSSERTHRGLUASNMET
23   PROILELYSILEASNLEUILEALAPROPRO
24   ARGTYRVALMETTHRTHRTHRTHRLEUGLU
25   ARGTHRGLUGLYLEUSERVALLEUSERGLN
26   ALAMETALAVALILELYSGLULYSILEGLU
27   GLULYSARGGLYVALPHEASNVALGLNMET
28   GLUPROLYSVALVALTHRASPTHRASPGLU
29   THRGLULEUALAARGGLNMETGLUARGLEU
30   GLUARGGLUASNALAGLUVALASPGLYASP
31   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: eIF2alpha, [U-2H; U-13C; U-15N], 0.5 – 0.7 mM

condition_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 10023
PDB
DBJ BAB27049 BAE25400 BAE39796 BAE89774 BAG35351
EMBL CAA37728 CAD61953 CAH93423
GB AAA41110 AAA52373 AAH02513 AAH05463 AAH16448
REF NP_001127006 NP_001244395 NP_001270066 NP_004085 NP_062229
SP P05198 P68101 P68102 Q5R493 Q6ZWX6
TPG DAA25059
AlphaFold P05198 P68101 P68102 Q5R493 Q6ZWX6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks