BMRB Entry 10023

Name: Chemical shift assignment of human eIF2alpha
Published: 2006-12-04

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10023

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shift assignment of human eIF2alpha

Deposition date:

2006-08-01

Original release date:

2006-12-04

Authors:

Ito, Takuhiro; Wagner, Gerhard

Citation:

Citation: Ito, Takuhiro; Wagner, Gerhard. "Resonance assignments of the alpha subunit of human eukaryotic initiation factor 2 (heIF2alpha)" J. Biomol. NMR 38, 173-173 (2007).
PubMed: 17122952

Assembly members:

Assembly members:
heIF2alpha, polymer, 308 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
heIF2alpha: MSCRFYQHKFPEVEDVVMVN VRSIQEMGAYVSLLEYNNIE GMIHLSELSRRRIRSINKLI RIGRNECVKVIRVDKEKGYI DLSKRRVSPEEAIKCEDKFT KSKTVYSILRHVAEVLEYTK DEQLESLFQRTAWVFDDKYK RPGYGAYDAFKHAVSDPSIL DSLDLNEDEREVLINNINRR LTPQAVKIRADIEVACYGYE GIDAVKEALRAGLNCSTENM PIKINLIAPPRYVMTTTTLE RTEGLSVLSQAMAVIKEKIE EKRGVFNVQMEPKVVTDTDE TELARQMERLERENAEVDGD LEHHHHHH

Data sets:

assigned_chemical_shifts
- eIF2alpha_chem_shifts

Data type	Count
13C chemical shifts	910
15N chemical shifts	282
1H chemical shifts	753

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	se-heIF2alpha	1

Entities:

Entity 1, se-heIF2alpha 308 residues - Formula weight is not available

1

MET

SER

CYS

ARG

PHE

TYR

GLN

HIS

LYS

PHE

2

PRO

GLU

VAL

GLU

ASP

VAL

MET

VAL

ASN

3

VAL

ARG

SER

ILE

GLN

GLU

MET

GLY

ALA

TYR

4

VAL

SER

LEU

GLU

TYR

ASN

ILE

GLU

5

GLY

MET

ILE

HIS

LEU

SER

GLU

LEU

SER

ARG

6

ARG

ILE

ARG

SER

ILE

ASN

LYS

LEU

ILE

7

ARG

ILE

GLY

ARG

ASN

GLU

CYS

VAL

LYS

VAL

8

ILE

ARG

VAL

ASP

LYS

GLU

LYS

GLY

TYR

ILE

9

ASP

LEU

SER

LYS

ARG

VAL

SER

PRO

GLU

10

GLU

ALA

ILE

LYS

CYS

GLU

ASP

LYS

PHE

THR

11

LYS

SER

LYS

THR

VAL

TYR

SER

ILE

LEU

ARG

12

HIS

VAL

ALA

GLU

VAL

LEU

GLU

TYR

THR

LYS

13

ASP

GLU

GLN

LEU

GLU

SER

LEU

PHE

GLN

ARG

14

THR

ALA

TRP

VAL

PHE

ASP

LYS

TYR

LYS

15

ARG

PRO

GLY

TYR

GLY

ALA

TYR

ASP

ALA

PHE

16

LYS

HIS

ALA

VAL

SER

ASP

PRO

SER

ILE

LEU

17

ASP

SER

LEU

ASP

LEU

ASN

GLU

ASP

GLU

ARG

18

GLU

VAL

LEU

ILE

ASN

ILE

ASN

ARG

19

LEU

THR

PRO

GLN

ALA

VAL

LYS

ILE

ARG

ALA

20

ASP

ILE

GLU

VAL

ALA

CYS

TYR

GLY

TYR

GLU

21

GLY

ILE

ASP

ALA

VAL

LYS

GLU

ALA

LEU

ARG

22

ALA

GLY

LEU

ASN

CYS

SER

THR

GLU

ASN

MET

23

PRO

ILE

LYS

ILE

ASN

LEU

ILE

ALA

PRO

24

ARG

TYR

VAL

MET

THR

LEU

GLU

25

ARG

THR

GLU

GLY

LEU

SER

VAL

LEU

SER

GLN

26

ALA

MET

ALA

VAL

ILE

LYS

GLU

LYS

ILE

GLU

27

GLU

LYS

ARG

GLY

VAL

PHE

ASN

VAL

GLN

MET

28

GLU

PRO

LYS

VAL

THR

ASP

THR

ASP

GLU

29

THR

GLU

LEU

ALA

ARG

GLN

MET

GLU

ARG

LEU

30

GLU

ARG

GLU

ASN

ALA

GLU

VAL

ASP

GLY

ASP

31

LEU

GLU

HIS

Samples:

sample_1: heIF2alpha, [U-2H; U-13C; U-15N], 0.5 ± 0.05 mM

sample_2: heIF2alpha, [NA-I,L,V-Me; U-2H; 13C-I,L,V; U-15N], 0.5 ± 0.05 mM

sample_3: heIF2alpha, [NA-I,L,V-Me; NA-F,Y; U-2H; 13C-I,L,V-Me; U-15N], 0.5 ± 0.05 mM

sample_4: heIF2alpha, [NA-F; U-2H], 0.5 ± 0.05 mM

sample_5: heIF2alpha, [NA-Y; U-2H], 0.5 ± 0.05 mM

condition_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	not available
2D 1H-13C HSQC	not available	not available	not available
3D TROSY-HNCO	not available	not available	not available
3D TROSY-HN(CA)CO	not available	not available	not available
3D TROSY-HNCA	not available	not available	not available
3D TROSY-HN(CO)CA	not available	not available	not available
3D TROSY-HN(CA)CB	not available	not available	not available
3D TROSY-HN(COCA)CB	not available	not available	not available
3D 15N-NOESY-HSQC	not available	not available	not available
3D TROSY-Me-H(CCO)NH	not available	not available	not available
3D TROSY-Me-C(CO)NH	not available	not available	not available
3D Me-HCCH-TOCSY	not available	not available	not available
3D Me-HMQC-NOESY	not available	not available	not available
4D Me-HMQC-NOESY-Me-HMQC	not available	not available	not available
2D NOESY for aromatic	not available	not available	not available
2D TOCSY for aromatic	not available	not available	not available

Software:

No software information available

NMR spectrometers:

CMR G 750 MHz
Varian Inova 750 MHz
Bruker Avance 600 MHz
Bruker Avance 500 MHz
Varian Inova 500 MHz

PDB	1Q8K
DBJ	BAB27049 BAE25400 BAE39796 BAE89774 BAG35351
EMBL	CAA37728 CAD61953 CAH93423
GB	AAA41110 AAA52373 AAH02513 AAH05463 AAH16448
REF	NP_001127006 NP_001244395 NP_001270066 NP_004085 NP_062229
SP	P05198 P68101 P68102 Q5R493 Q6ZWX6
TPG	DAA25059
AlphaFold	P05198 P68101 P68102 Q5R493 Q6ZWX6