BMRB Entry 5910
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5910
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of core hydrophobicity and packing in the hyperthermophile
proteins Sac7d and Sso7d." Biochemistry 43, 2840-2853 (2004).
PubMed: 15005619
Assembly members:
Sso7d, polymer, 64 residues, 7265 Da.
Natural source: Common Name: Sulfolobus acidocaldarius Brock et al Taxonomy ID: 2285 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus acidocaldarius
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Sso7d: MATVKFKYKGEEKQVDISKI
KKVWRVGKMVSFTYDEGGGK
TGRGAVSEKDAPKELLQMLE
KQKK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 62 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sso7d monomer | 1 |
Entities:
Entity 1, Sso7d monomer 64 residues - 7265 Da.
| 1 | MET | ALA | THR | VAL | LYS | PHE | LYS | TYR | LYS | GLY | ||||
| 2 | GLU | GLU | LYS | GLN | VAL | ASP | ILE | SER | LYS | ILE | ||||
| 3 | LYS | LYS | VAL | TRP | ARG | VAL | GLY | LYS | MET | VAL | ||||
| 4 | SER | PHE | THR | TYR | ASP | GLU | GLY | GLY | GLY | LYS | ||||
| 5 | THR | GLY | ARG | GLY | ALA | VAL | SER | GLU | LYS | ASP | ||||
| 6 | ALA | PRO | LYS | GLU | LEU | LEU | GLN | MET | LEU | GLU | ||||
| 7 | LYS | GLN | LYS | LYS |
Samples:
I30V_sample_1: Sso7d, [U-98% 15N], 1.28 mM; H2O 90%; D2O 10%; DSS 100 uM
pH5_0salt: pH: 5.07 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,15N HSQC | I30V_sample_1 | not available | pH5_0salt |
| 3D HSQC-TOCSY | I30V_sample_1 | not available | pH5_0salt |
| 3D HSQC-NOESY | I30V_sample_1 | not available | pH5_0salt |
Software:
NMRPipe - data processing, referencing, transformation
NMRView v5 - sequential assignments
NMR spectrometers:
- Varian VXR-S 500 MHz
- Varian INOVA 800 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks