BMRB Entry 5756

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5756

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Title:
Resonance Assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA
Deposition date:
2003-03-26
Original release date:
2004-04-23
Authors:
Liu, Gaohua; Mills, Jeffrey; Hess, Tracy; Kim, Seho; Skalicky, Jack; Sukumaran, Dinesh; Kupce, Eriks; Skerra, Arne; Szyperski, Thomas
Citation:

Citation: Liu, Gaohua; Mills, Jeffrey; Hess, Tracy; Kim, Seho; Skalicky, Jack; Sukumaran, Dinesh; Kupce, Eriks; Skerra, Arne; Szyperski, Thomas. "Letter to the Editor: Resonace Assignments for the 21 kDa engineered fluorescein-binding lipocalin FluA"  J. Biomol. NMR 27, 187-188 (2003).

Assembly members:

Assembly members:
fluorescein-bind lipocalin, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cabbage butterfly   Taxonomy ID: 7116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pieris brassicae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fluorescein-bind lipocalin: DVYHDGACPEVKPVDNFDWS QYHGKWWEVAKYPSPNGKYG KCGWAEYTPEGKSVKVSRYD VIHGKEYFMEGTAYPVGDSK IGKIYHSRTVGGYTKKTVFN VLSTDNKNYIIGYSCRYDED KKGHWDHVWVLSRSMVLTGE AKTAVENYLIGSPVVDSQKL VYSDFSEAACKVNNSNWSHP QFEK

Data sets:
Data typeCount
13C chemical shifts809
15N chemical shifts185
1H chemical shifts1236

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FluA subunit1, FluA monomer1

Entities:

Entity 1, FluA subunit1, FluA monomer 184 residues - Formula weight is not available

1   ASPVALTYRHISASPGLYALACYSPROGLU
2   VALLYSPROVALASPASNPHEASPTRPSER
3   GLNTYRHISGLYLYSTRPTRPGLUVALALA
4   LYSTYRPROSERPROASNGLYLYSTYRGLY
5   LYSCYSGLYTRPALAGLUTYRTHRPROGLU
6   GLYLYSSERVALLYSVALSERARGTYRASP
7   VALILEHISGLYLYSGLUTYRPHEMETGLU
8   GLYTHRALATYRPROVALGLYASPSERLYS
9   ILEGLYLYSILETYRHISSERARGTHRVAL
10   GLYGLYTYRTHRLYSLYSTHRVALPHEASN
11   VALLEUSERTHRASPASNLYSASNTYRILE
12   ILEGLYTYRSERCYSARGTYRASPGLUASP
13   LYSLYSGLYHISTRPASPHISVALTRPVAL
14   LEUSERARGSERMETVALLEUTHRGLYGLU
15   ALALYSTHRALAVALGLUASNTYRLEUILE
16   GLYSERPROVALVALASPSERGLNLYSLEU
17   VALTYRSERASPPHESERGLUALAALACYS
18   LYSVALASNASNSERASNTRPSERHISPRO
19   GLNPHEGLULYS

Samples:

sample1: fluorescein-bind lipocalin, [U-95% 13C; U-90% 15N], 0.7 mM

Sample2: fluorescein-bind lipocalin, [U-90% 15N], 0.7 mM

sample3: fluorescein-bind lipocalin, [U-50% 2H; U-90% 15N], 0.7 mM

Ex-cond1: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSYnot availablenot availablenot available
HNNCACBnot availablenot availablenot available
CBCA(CO)NHNnot availablenot availablenot available
HC(C)HCOSYnot availablenot availablenot available
HC(C)HTOCSYnot availablenot availablenot available
RD HNNCAHAnot availablenot availablenot available
RD HACA(CO)NHNnot availablenot availablenot available
RD HBCBHACA(CO)NHNnot availablenot availablenot available
RD HBCB(CGCD)HDnot availablenot availablenot available
RD 1H-TOCSY relayed HCH COSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N NOESY-TROSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 6182
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks