BMRB Entry 6182

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6182

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Title:
Backbone assignments and 15N spin-relaxation rates of Holo-FluA(R95K)
Deposition date:
2004-04-12
Original release date:
2007-08-27
Authors:
Mills, Jeffrey; Szyperski, Thomas
Citation:

Citation: Mills, Jeffrey; Liu, Gaohua; Skerra, Arne; Szyperski, Thomas. "1H, 13C, and 15N backbone chemical shifts and 15N spin-relaxation rates for Holo-FluA(R95K)"  J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
FluA, polymer, 184 residues, 332.306 Da.
FLU, non-polymer, 332.306 Da.

Natural source:

Natural source:   Common Name: cabbage butterfly   Taxonomy ID: 7116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pieris brassicae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FluA: DVYHDGACPEVKPVDNFDWS QYHGKWWEVAKYPSPNGKYG KCGWAEYTPEGKSVKVSRYD VIHGKEYFMEGTAYPVGDSK IGKIYHSRTVGGYTKKTVFN VLSTDNKNYIIGYSCRYDED KKGHWDHVWVLSRSMVLTGE AKTAVENYLIGSPVVDSQKL VYSDFSEAACKVNNSNWSHP QFEK

Data sets:
Data typeCount
13C chemical shifts510
15N chemical shifts176
1H chemical shifts176
T1 relaxation values145
T2 relaxation values145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FluA(R95K)1
22-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-BENZOIC ACID2

Entities:

Entity 1, FluA(R95K) 184 residues - 332.306 Da.

1   ASPVALTYRHISASPGLYALACYSPROGLU
2   VALLYSPROVALASPASNPHEASPTRPSER
3   GLNTYRHISGLYLYSTRPTRPGLUVALALA
4   LYSTYRPROSERPROASNGLYLYSTYRGLY
5   LYSCYSGLYTRPALAGLUTYRTHRPROGLU
6   GLYLYSSERVALLYSVALSERARGTYRASP
7   VALILEHISGLYLYSGLUTYRPHEMETGLU
8   GLYTHRALATYRPROVALGLYASPSERLYS
9   ILEGLYLYSILETYRHISSERARGTHRVAL
10   GLYGLYTYRTHRLYSLYSTHRVALPHEASN
11   VALLEUSERTHRASPASNLYSASNTYRILE
12   ILEGLYTYRSERCYSARGTYRASPGLUASP
13   LYSLYSGLYHISTRPASPHISVALTRPVAL
14   LEUSERARGSERMETVALLEUTHRGLYGLU
15   ALALYSTHRALAVALGLUASNTYRLEUILE
16   GLYSERPROVALVALASPSERGLNLYSLEU
17   VALTYRSERASPPHESERGLUALAALACYS
18   LYSVALASNASNSERASNTRPSERHISPRO
19   GLNPHEGLULYS

Entity 2, 2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-BENZOIC ACID - C20 H12 O5 - 332.306 Da.

1   FLU

Samples:

sample_U-13C_15N_FluA(R95K): FluA, [U-13C; U-15N], 700 uM; sodium chloride 150 mM; sodium phosphate 10 mM; EDTA 200 uM; benzamidine 50 mM

sample_50%_2H_U-15N_FluA(R95K): FluA, [U-50% 2H; U-15N], 700 uM; sodium chloride 150 mM; sodium phosphate 10 mM; EDTA 200 uM; benzamidine 50 mM

Ex-cond_1: pH*: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
[15N, 1H]-HSQCnot availablenot availableEx-cond_1
HNNCOnot availablenot availableEx-cond_1
HNNCAnot availablenot availableEx-cond_1
HNN(CO)CAnot availablenot availableEx-cond_1
HNNCACBnot availablenot availableEx-cond_1
HNN(CO)CACBnot availablenot availableEx-cond_1
15N{1H}-NOEnot availablenot availableEx-cond_1
15N T1not availablenot availableEx-cond_1
15N T2not availablenot availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

BMRB 5756
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks