BMRB Entry 5511

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5511
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N resonance assignments for the 21 kDa GTPase Rac1 complexed to GDP and Mg2+
Deposition date:
2002-08-28
Original release date:
2005-11-14
Authors:
Thapar, Roopa; Moore, Cathy; Campbell, Sharon
Citation:

Citation: Thapar, Roopa; Moore, Cathy; Campbell, Sharon. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments for the 21 kDa GTPase Rac1 complexed to GDP and Mg2+"  J. Biomol. NMR 27, 87-88 (2003).

Assembly members:

Assembly members:
Rac1, polymer, 188 residues, 20991.4 Da.
GDP, non-polymer, 443.201 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rac1: MQAIKCVVVGDGAVGKTCLL ISYTTNAFPGEYIPTVFDNY SANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLI CFSLVSPASFENVRAKWYPE VRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLTPITYP QGLAMAKEIGAVKYLECSAL TQRGLKTVFDEAIRAVLSPP PVKKRKRK

Data sets:
Data typeCount
1H chemical shifts138
13C chemical shifts261
15N chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rac11
2Guanosine-5'-diphosphate2
3Magnesium ion3

Entities:

Entity 1, Rac1 188 residues - 20991.4 Da.

1   METGLNALAILELYSCYSVALVALVALGLY
2   ASPGLYALAVALGLYLYSTHRCYSLEULEU
3   ILESERTYRTHRTHRASNALAPHEPROGLY
4   GLUTYRILEPROTHRVALPHEASPASNTYR
5   SERALAASNVALMETVALASPGLYLYSPRO
6   VALASNLEUGLYLEUTRPASPTHRALAGLY
7   GLNGLUASPTYRASPARGLEUARGPROLEU
8   SERTYRPROGLNTHRASPVALPHELEUILE
9   CYSPHESERLEUVALSERPROALASERPHE
10   GLUASNVALARGALALYSTRPTYRPROGLU
11   VALARGHISHISCYSPROASNTHRPROILE
12   ILELEUVALGLYTHRLYSLEUASPLEUARG
13   ASPASPLYSASPTHRILEGLULYSLEULYS
14   GLULYSLYSLEUTHRPROILETHRTYRPRO
15   GLNGLYLEUALAMETALALYSGLUILEGLY
16   ALAVALLYSTYRLEUGLUCYSSERALALEU
17   THRGLNARGGLYLEULYSTHRVALPHEASP
18   GLUALAILEARGALAVALLEUSERPROPRO
19   PROVALLYSLYSARGLYSARGLYS

Entity 2, Guanosine-5'-diphosphate - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, Magnesium ion - Mg - 24.305 Da.

1   MG

Samples:

Rac1_GDP_Mg_(15N_13C_2H): Rac1, [U-15N; U-13C; U-2H], 0.5 – 0.7 mM

condition_1: pH: 6.8; temperature: 298 K; ionic strength: 0.065 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HNCARac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HN(CO)CARac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HNCORac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
1H-15N TOCSY-HSQCRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
CBCA(CO)NHRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
HNCACBRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
CC(CO)NH TOCSYRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1
1H-15N NOESY-HSQCRac1_GDP_Mg_(15N_13C_2H)not availablecondition_1

Software:

FELIX v98.2 - data processing, analysis

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

SWISS-PROT Q6RUV5 P63001 P63000 P62999 P62998
REF NP_001089332 NP_001084224 NP_001034907 NP_001004840 NP_001003274
GenBank AAD50299 AAC18960 AAB22206 AAA36544 AAA36537
EMBL CAJ83626 CAG04437 CAB53579 CAA40545 CAA39801
DBJ BAC28767 BAC16311 BAB69451 BAB26027 BAB25667
PDB
BMRB 6970 11010

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks