BMRB Entry 6970

Name: 1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1
Published: 2006-08-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6970

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1

Deposition date:

2006-02-06

Original release date:

2006-08-07

Authors:

Bouguet-Bonnet, Sabine; Buck, Matthias

Citation:

Citation: Bouguet-Bonnet, Sabine; Buck, Matthias. "1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1" J. Biomol. NMR 36, 51-51 (2006).
PubMed: 16858624

Assembly members:

Assembly members:
Rac1, polymer, 183 residues, Formula weight is not available
GTP, non-polymer, 523.180 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rac1: MQAIKCVVVGDGAVGKTCLL ISYTTNAFPGEYIPTVFDNY SANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLI CFSLVSPASFENVRAKWYPE VRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLTPITYP QGLAMAKEIGAVKYLECSAL TQRGLKTVFDEAIRAVLSPP PVK

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	576
15N chemical shifts	141
1H chemical shifts	141

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rac1	1
2	MAGNESIUM (II) ION	3
3	guanosine-5'[(b,g)-imido]triphosphate	2

Entities:

Entity 1, Rac1 183 residues - Formula weight is not available

1

MET

GLN

ALA

ILE

LYS

CYS

VAL

GLY

2

ASP

GLY

ALA

VAL

GLY

LYS

THR

CYS

LEU

3

ILE

SER

TYR

THR

ASN

ALA

PHE

PRO

GLY

4

GLU

TYR

ILE

PRO

THR

VAL

PHE

ASP

ASN

TYR

5

SER

ALA

ASN

VAL

MET

VAL

ASP

GLY

LYS

PRO

6

VAL

ASN

LEU

GLY

LEU

TRP

ASP

THR

ALA

GLY

7

GLN

GLU

ASP

TYR

ASP

ARG

LEU

ARG

PRO

LEU

8

SER

TYR

PRO

GLN

THR

ASP

VAL

PHE

LEU

ILE

9

CYS

PHE

SER

LEU

VAL

SER

PRO

ALA

SER

PHE

10

GLU

ASN

VAL

ARG

ALA

LYS

TRP

TYR

PRO

GLU

11

VAL

ARG

HIS

CYS

PRO

ASN

THR

PRO

ILE

12

ILE

LEU

VAL

GLY

THR

LYS

LEU

ASP

LEU

ARG

13

ASP

LYS

ASP

THR

ILE

GLU

LYS

LEU

LYS

14

GLU

LYS

LEU

THR

PRO

ILE

THR

TYR

PRO

15

GLN

GLY

LEU

ALA

MET

ALA

LYS

GLU

ILE

GLY

16

ALA

VAL

LYS

TYR

LEU

GLU

CYS

SER

ALA

LEU

17

THR

GLN

ARG

GLY

LEU

LYS

THR

VAL

PHE

ASP

18

GLU

ALA

ILE

ARG

ALA

VAL

LEU

SER

PRO

19

PRO

VAL

LYS

Entity 3, MAGNESIUM (II) ION - Mg - 24.305 Da.

1

MG

Entity 2, guanosine-5'[(b,g)-imido]triphosphate - C10 H16 N5 O14 P3 - 523.180 Da.

1

GTP

Samples:

sample_1: Rac1, [U-15N; U-13C], 0.6 ± 0.05 mM

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
HN(CO)CA	sample_1	not available	conditions_1
HNCO	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
CC(CO)NH	sample_1	not available	conditions_1

Software:

NMRView v5.2.2 - Spectral analysis

NMRPipe v2.3 - Data processing

NMR spectrometers:

Bruker DRX 800 MHz

BMRB	5511
PDB	1E96 1FOE 1G4U 1HE1 1HH4 1I4D 1I4L 1I4T 1MH1 2FJU 2H7V 2NZ8 2P2L 2RMK 2VRW 2YIN 3B13 3BJI 3RYT 3SBD 3SBE 3SU8 3SUA 3TH5 4GZL 4GZM
DBJ	BAB25667 BAB26027 BAB69451 BAC16311 BAC28767
EMBL	CAA39801 CAA40545 CAB53579 CAG04437 CAJ83626
GB	AAA36537 AAA36544 AAB22206 AAC18960 AAD50299
REF	NP_001003274 NP_001004840 NP_001034907 NP_001084224 NP_001089332
SP	P62998 P62999 P63000 P63001 Q6RUV5
TPG	DAA15019
AlphaFold	P62998 P62999 P63000 P63001 Q6RUV5