BMRB Entry 6970

Title:
1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1
Deposition date:
2006-02-06
Original release date:
2006-08-07
Authors:
Bouguet-Bonnet, Sabine; Buck, Matthias
Citation:

Citation: Bouguet-Bonnet, Sabine; Buck, Matthias. "1H, 15N, 13C assignments for the activated form of the small Rho-GTPase Rac1"  J. Biomol. NMR 36, 51-51 (2006).
PubMed: 16858624

Assembly members:

Assembly members:
Rac1, polymer, 183 residues, Formula weight is not available
GTP, non-polymer, 523.180 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts576
15N chemical shifts141
1H chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rac11
2MAGNESIUM (II) ION3
3guanosine-5'[(b,g)-imido]triphosphate2

Entities:

Entity 1, Rac1 183 residues - Formula weight is not available

1   METGLNALAILELYSCYSVALVALVALGLY
2   ASPGLYALAVALGLYLYSTHRCYSLEULEU
3   ILESERTYRTHRTHRASNALAPHEPROGLY
4   GLUTYRILEPROTHRVALPHEASPASNTYR
5   SERALAASNVALMETVALASPGLYLYSPRO
6   VALASNLEUGLYLEUTRPASPTHRALAGLY
7   GLNGLUASPTYRASPARGLEUARGPROLEU
8   SERTYRPROGLNTHRASPVALPHELEUILE
9   CYSPHESERLEUVALSERPROALASERPHE
10   GLUASNVALARGALALYSTRPTYRPROGLU
11   VALARGHISHISCYSPROASNTHRPROILE
12   ILELEUVALGLYTHRLYSLEUASPLEUARG
13   ASPASPLYSASPTHRILEGLULYSLEULYS
14   GLULYSLYSLEUTHRPROILETHRTYRPRO
15   GLNGLYLEUALAMETALALYSGLUILEGLY
16   ALAVALLYSTYRLEUGLUCYSSERALALEU
17   THRGLNARGGLYLEULYSTHRVALPHEASP
18   GLUALAILEARGALAVALLEUSERPROPRO
19   PROVALLYS

Entity 3, MAGNESIUM (II) ION - Mg - 24.305 Da.

1   MG

Entity 2, guanosine-5'[(b,g)-imido]triphosphate - C10 H16 N5 O14 P3 - 523.180 Da.

1   GTP

Samples:

sample_1: Rac1, [U-15N; U-13C], 0.6 ± 0.05 mM

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
CC(CO)NHsample_1not availableconditions_1

Software:

NMRView v5.2.2 - Spectral analysis

NMRPipe v2.3 - Data processing

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 5511
PDB
DBJ BAB25667 BAB26027 BAB69451 BAC16311 BAC28767
EMBL CAA39801 CAA40545 CAB53579 CAG04437 CAJ83626
GB AAA36537 AAA36544 AAB22206 AAC18960 AAD50299
REF NP_001003274 NP_001004840 NP_001034907 NP_001084224 NP_001089332
SP P62998 P62999 P63000 P63001 Q6RUV5
TPG DAA15019
AlphaFold P62998 P62999 P63000 P63001 Q6RUV5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks